Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives

The neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here,...

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Autores principales:	Ezra Lencer, Rytis Prekeris, Kristin Bruk Artinger
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	rohon-beard cells xanthophores pigment development neural crest cells scRNA-seq Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3a4c89aed1704fbd9e8a1d27e084afda
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spelling	oai:doaj.org-article:3a4c89aed1704fbd9e8a1d27e084afda2021-11-17T10:50:21ZSingle-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives10.7554/eLife.660782050-084Xe66078https://doaj.org/article/3a4c89aed1704fbd9e8a1d27e084afda2021-08-01T00:00:00Zhttps://elifesciences.org/articles/66078https://doaj.org/toc/2050-084XThe neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here, we use single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. We show that neural crest cells are transcriptionally diverse and identify pre-migratory populations already expressing genes associated with differentiated derivatives, specifically in the xanthophore lineage. Further, we identify a population of Rohon–Beard neurons in the data. The data presented identify novel genetic markers for multiple trunk neural crest cell populations and Rohon–Beard neurons providing insight into previously uncharacterized genes critical for vertebrate development.Ezra LencerRytis PrekerisKristin Bruk ArtingereLife Sciences Publications Ltdarticlerohon-beard cellsxanthophorespigment developmentneural crest cellsscRNA-seqMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rohon-beard cells xanthophores pigment development neural crest cells scRNA-seq Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	rohon-beard cells xanthophores pigment development neural crest cells scRNA-seq Medicine R Science Q Biology (General) QH301-705.5 Ezra Lencer Rytis Prekeris Kristin Bruk Artinger Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
description	The neural crest is a migratory population of stem-like cells that contribute to multiple traits including the bones of the skull, peripheral nervous system, and pigment. How neural crest cells differentiate into diverse cell types is a fundamental question in the study of vertebrate biology. Here, we use single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. We show that neural crest cells are transcriptionally diverse and identify pre-migratory populations already expressing genes associated with differentiated derivatives, specifically in the xanthophore lineage. Further, we identify a population of Rohon–Beard neurons in the data. The data presented identify novel genetic markers for multiple trunk neural crest cell populations and Rohon–Beard neurons providing insight into previously uncharacterized genes critical for vertebrate development.
format	article
author	Ezra Lencer Rytis Prekeris Kristin Bruk Artinger
author_facet	Ezra Lencer Rytis Prekeris Kristin Bruk Artinger
author_sort	Ezra Lencer
title	Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
title_short	Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
title_full	Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
title_fullStr	Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
title_full_unstemmed	Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
title_sort	single-cell rna analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/3a4c89aed1704fbd9e8a1d27e084afda
work_keys_str_mv	AT ezralencer singlecellrnaanalysisidentifiespremigratoryneuralcrestcellsexpressingmarkersofdifferentiatedderivatives AT rytisprekeris singlecellrnaanalysisidentifiespremigratoryneuralcrestcellsexpressingmarkersofdifferentiatedderivatives AT kristinbrukartinger singlecellrnaanalysisidentifiespremigratoryneuralcrestcellsexpressingmarkersofdifferentiatedderivatives
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Single-cell RNA analysis identifies pre-migratory neural crest cells expressing markers of differentiated derivatives

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