Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.

We hypothesized that evolution of salivary gland secretory proteome has been important in adaptation to insectivory, the most common dietary strategy among Chiroptera. A submandibular salivary gland (SMG) transcriptome was sequenced for the little brown bat, Myotis lucifugus. The likely secretory pr...

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Autores principales:	Carleton J Phillips, Caleb D Phillips, Jeremy Goecks, Enrique P Lessa, Cibele G Sotero-Caio, Bernard Tandler, Michael R Gannon, Robert J Baker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a4d2495ec924a85a5d9ed232ba63114
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spelling	oai:doaj.org-article:3a4d2495ec924a85a5d9ed232ba631142021-11-18T08:37:53ZDietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.1932-620310.1371/journal.pone.0083512https://doaj.org/article/3a4d2495ec924a85a5d9ed232ba631142014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24454705/?tool=EBIhttps://doaj.org/toc/1932-6203We hypothesized that evolution of salivary gland secretory proteome has been important in adaptation to insectivory, the most common dietary strategy among Chiroptera. A submandibular salivary gland (SMG) transcriptome was sequenced for the little brown bat, Myotis lucifugus. The likely secretory proteome of 23 genes included seven (RETNLB, PSAP, CLU, APOE, LCN2, C3, CEL) related to M. lucifugus insectivorous diet and metabolism. Six of the secretory proteins probably are endocrine, whereas one (CEL) most likely is exocrine. The encoded proteins are associated with lipid hydrolysis, regulation of lipid metabolism, lipid transport, and insulin resistance. They are capable of processing exogenous lipids for flight metabolism while foraging. Salivary carboxyl ester lipase (CEL) is thought to hydrolyze insect lipophorins, which probably are absorbed across the gastric mucosa during feeding. The other six proteins are predicted either to maintain these lipids at high blood concentrations or to facilitate transport and uptake by flight muscles. Expression of these seven genes and coordinated secretion from a single organ is novel to this insectivorous bat, and apparently has evolved through instances of gene duplication, gene recruitment, and nucleotide selection. Four of the recruited genes are single-copy in the Myotis genome, whereas three have undergone duplication(s) with two of these genes exhibiting evolutionary 'bursts' of duplication resulting in multiple paralogs. Evidence for episodic directional selection was found for six of seven genes, reinforcing the conclusion that the recruited genes have important roles in adaptation to insectivory and the metabolic demands of flight. Intragenic frequencies of mobile- element-like sequences differed from frequencies in the whole M. lucifugus genome. Differences among recruited genes imply separate evolutionary trajectories and that adaptation was not a single, coordinated event.Carleton J PhillipsCaleb D PhillipsJeremy GoecksEnrique P LessaCibele G Sotero-CaioBernard TandlerMichael R GannonRobert J BakerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e83512 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Carleton J Phillips Caleb D Phillips Jeremy Goecks Enrique P Lessa Cibele G Sotero-Caio Bernard Tandler Michael R Gannon Robert J Baker Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
description	We hypothesized that evolution of salivary gland secretory proteome has been important in adaptation to insectivory, the most common dietary strategy among Chiroptera. A submandibular salivary gland (SMG) transcriptome was sequenced for the little brown bat, Myotis lucifugus. The likely secretory proteome of 23 genes included seven (RETNLB, PSAP, CLU, APOE, LCN2, C3, CEL) related to M. lucifugus insectivorous diet and metabolism. Six of the secretory proteins probably are endocrine, whereas one (CEL) most likely is exocrine. The encoded proteins are associated with lipid hydrolysis, regulation of lipid metabolism, lipid transport, and insulin resistance. They are capable of processing exogenous lipids for flight metabolism while foraging. Salivary carboxyl ester lipase (CEL) is thought to hydrolyze insect lipophorins, which probably are absorbed across the gastric mucosa during feeding. The other six proteins are predicted either to maintain these lipids at high blood concentrations or to facilitate transport and uptake by flight muscles. Expression of these seven genes and coordinated secretion from a single organ is novel to this insectivorous bat, and apparently has evolved through instances of gene duplication, gene recruitment, and nucleotide selection. Four of the recruited genes are single-copy in the Myotis genome, whereas three have undergone duplication(s) with two of these genes exhibiting evolutionary 'bursts' of duplication resulting in multiple paralogs. Evidence for episodic directional selection was found for six of seven genes, reinforcing the conclusion that the recruited genes have important roles in adaptation to insectivory and the metabolic demands of flight. Intragenic frequencies of mobile- element-like sequences differed from frequencies in the whole M. lucifugus genome. Differences among recruited genes imply separate evolutionary trajectories and that adaptation was not a single, coordinated event.
format	article
author	Carleton J Phillips Caleb D Phillips Jeremy Goecks Enrique P Lessa Cibele G Sotero-Caio Bernard Tandler Michael R Gannon Robert J Baker
author_facet	Carleton J Phillips Caleb D Phillips Jeremy Goecks Enrique P Lessa Cibele G Sotero-Caio Bernard Tandler Michael R Gannon Robert J Baker
author_sort	Carleton J Phillips
title	Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
title_short	Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
title_full	Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
title_fullStr	Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
title_full_unstemmed	Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
title_sort	dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3a4d2495ec924a85a5d9ed232ba63114
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Dietary and flight energetic adaptations in a salivary gland transcriptome of an insectivorous bat.

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