Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A met...

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Autores principales:	Gyu Seong Yeom, In-ho Song, Shrikant Dashrath Warkad, Pramod B. Shinde, Taewoon Kim, Seong-min Park, Satish Balasaheb Nimse
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cysteine biothiols cystinuria portable fluorimeter bio-imaging Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/3a504168208f4fa980395abf00c82ae2
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spelling	oai:doaj.org-article:3a504168208f4fa980395abf00c82ae22021-11-25T16:54:58ZDevelopment of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine10.3390/bios111104202079-6374https://doaj.org/article/3a504168208f4fa980395abf00c82ae22021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6374/11/11/420https://doaj.org/toc/2079-6374The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (<b>ABIA</b>) along with the design and development of a portable fluorescence spectrometer device (<b>CysDDev</b>) for detecting cysteine in simulated human urine. <b>ABIA</b> showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed <b>CysDDev</b>. Further, <b>ABIA</b> also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.Gyu Seong YeomIn-ho SongShrikant Dashrath WarkadPramod B. ShindeTaewoon KimSeong-min ParkSatish Balasaheb NimseMDPI AGarticlecysteinebiothiolscystinuriaportablefluorimeterbio-imagingBiotechnologyTP248.13-248.65ENBiosensors, Vol 11, Iss 420, p 420 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cysteine biothiols cystinuria portable fluorimeter bio-imaging Biotechnology TP248.13-248.65
spellingShingle	cysteine biothiols cystinuria portable fluorimeter bio-imaging Biotechnology TP248.13-248.65 Gyu Seong Yeom In-ho Song Shrikant Dashrath Warkad Pramod B. Shinde Taewoon Kim Seong-min Park Satish Balasaheb Nimse Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
description	The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (<b>ABIA</b>) along with the design and development of a portable fluorescence spectrometer device (<b>CysDDev</b>) for detecting cysteine in simulated human urine. <b>ABIA</b> showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed <b>CysDDev</b>. Further, <b>ABIA</b> also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.
format	article
author	Gyu Seong Yeom In-ho Song Shrikant Dashrath Warkad Pramod B. Shinde Taewoon Kim Seong-min Park Satish Balasaheb Nimse
author_facet	Gyu Seong Yeom In-ho Song Shrikant Dashrath Warkad Pramod B. Shinde Taewoon Kim Seong-min Park Satish Balasaheb Nimse
author_sort	Gyu Seong Yeom
title	Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
title_short	Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
title_full	Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
title_fullStr	Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
title_full_unstemmed	Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine
title_sort	development of a novel benzimidazole-based probe and portable fluorimeter for the detection of cysteine in human urine
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3a504168208f4fa980395abf00c82ae2
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Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

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