Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands

Objective: Structural changes of rat submandibular glands (SMG) after long-term treatment with lead were studied. Methods: After using various doses of lead (0.01%, 0.04%, 0.05%), SMGs were removed and homogenized for measurement of total protein, DNA, calcium and lead concentrations. Also ultrastru...

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Autores principales:	M Abdollahi, Gh Abri, M sharif Zadeh, H Marzban, M Torab Jahromi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 1999
Materias:	lead chronic exposure submandibular glands dna protein structural changes Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3a505389e32347c6a6adf687bb227e7a
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spelling	oai:doaj.org-article:3a505389e32347c6a6adf687bb227e7a2021-11-10T09:24:24ZChronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands1561-41072251-7170https://doaj.org/article/3a505389e32347c6a6adf687bb227e7a1999-07-01T00:00:00Zhttp://jbums.org/article-1-2948-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170Objective: Structural changes of rat submandibular glands (SMG) after long-term treatment with lead were studied. Methods: After using various doses of lead (0.01%, 0.04%, 0.05%), SMGs were removed and homogenized for measurement of total protein, DNA, calcium and lead concentrations. Also ultrastructural examination of glands by electron microscopy (EM) was carried out. Findings: Total protein, DNA and intracellular calcium concentrations of treated glands at 0.04% and 0.05% lead concentrations showed significant reduction when compared with those of controls. Data of lead measurement as determined by atomic absorption spectroscopy showed that lead treatment resulted in accumulation of lead in rat’s SMGs. Lead treatment also caused degenerative changes in the cells. Dilation of rough endoplasmic reticulum (RER) and swollen mitochondria were observed in the (0.05%) treated group. Transformation and enlargement of mitochondria were also determined in the (0.04%) treated group. Conclusion: It is proposed that lead treatment can reduce exocytosis processes by the reduction in energy level of the cell and destruction of RER or mitochondria. Considering properties of lead to substitute for calcium in many intracellular events, different types of alterations may ensue, including interaction with protein and DNA synthesis. To elucidate the exact mechanisms of observed alterations further studies are needed to conduct.M AbdollahiGh AbriM sharif ZadehH MarzbanM Torab JahromiBabol University of Medical Sciencesarticleleadchronic exposuresubmandibular glandsdnaproteinstructural changesMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 1, Iss 3, Pp 29-36 (1999)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	lead chronic exposure submandibular glands dna protein structural changes Medicine R Medicine (General) R5-920
spellingShingle	lead chronic exposure submandibular glands dna protein structural changes Medicine R Medicine (General) R5-920 M Abdollahi Gh Abri M sharif Zadeh H Marzban M Torab Jahromi Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
description	Objective: Structural changes of rat submandibular glands (SMG) after long-term treatment with lead were studied. Methods: After using various doses of lead (0.01%, 0.04%, 0.05%), SMGs were removed and homogenized for measurement of total protein, DNA, calcium and lead concentrations. Also ultrastructural examination of glands by electron microscopy (EM) was carried out. Findings: Total protein, DNA and intracellular calcium concentrations of treated glands at 0.04% and 0.05% lead concentrations showed significant reduction when compared with those of controls. Data of lead measurement as determined by atomic absorption spectroscopy showed that lead treatment resulted in accumulation of lead in rat’s SMGs. Lead treatment also caused degenerative changes in the cells. Dilation of rough endoplasmic reticulum (RER) and swollen mitochondria were observed in the (0.05%) treated group. Transformation and enlargement of mitochondria were also determined in the (0.04%) treated group. Conclusion: It is proposed that lead treatment can reduce exocytosis processes by the reduction in energy level of the cell and destruction of RER or mitochondria. Considering properties of lead to substitute for calcium in many intracellular events, different types of alterations may ensue, including interaction with protein and DNA synthesis. To elucidate the exact mechanisms of observed alterations further studies are needed to conduct.
format	article
author	M Abdollahi Gh Abri M sharif Zadeh H Marzban M Torab Jahromi
author_facet	M Abdollahi Gh Abri M sharif Zadeh H Marzban M Torab Jahromi
author_sort	M Abdollahi
title	Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
title_short	Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
title_full	Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
title_fullStr	Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
title_full_unstemmed	Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands
title_sort	chronic exposure to lead and dna, protein & ultrastructural changes in rat submandibular glands
publisher	Babol University of Medical Sciences
publishDate	1999
url	https://doaj.org/article/3a505389e32347c6a6adf687bb227e7a
work_keys_str_mv	AT mabdollahi chronicexposuretoleadanddnaproteinultrastructuralchangesinratsubmandibularglands AT ghabri chronicexposuretoleadanddnaproteinultrastructuralchangesinratsubmandibularglands AT msharifzadeh chronicexposuretoleadanddnaproteinultrastructuralchangesinratsubmandibularglands AT hmarzban chronicexposuretoleadanddnaproteinultrastructuralchangesinratsubmandibularglands AT mtorabjahromi chronicexposuretoleadanddnaproteinultrastructuralchangesinratsubmandibularglands
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Chronic exposure to Lead and DNA, protein & ultrastructural changes in rat submandibular glands

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