Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

SpCas9 has a NGG PAM requirement that limits its DNA target range. Here the authors present a PAM-expanded SpdCas9 variant SpdNG-LWQT to target NRN PAMs.

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Autores principales:	Jian Wang, Yuxi Teng, Ruihua Zhang, Yifei Wu, Lei Lou, Yusong Zou, Michelle Li, Zhong-Ru Xie, Yajun Yan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3a51c52620b74b36aec06b2ae3c5ca95
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spelling	oai:doaj.org-article:3a51c52620b74b36aec06b2ae3c5ca952021-11-28T12:31:51ZEngineering a PAM-flexible SpdCas9 variant as a universal gene repressor10.1038/s41467-021-27290-92041-1723https://doaj.org/article/3a51c52620b74b36aec06b2ae3c5ca952021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-27290-9https://doaj.org/toc/2041-1723SpCas9 has a NGG PAM requirement that limits its DNA target range. Here the authors present a PAM-expanded SpdCas9 variant SpdNG-LWQT to target NRN PAMs.Jian WangYuxi TengRuihua ZhangYifei WuLei LouYusong ZouMichelle LiZhong-Ru XieYajun YanNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jian Wang Yuxi Teng Ruihua Zhang Yifei Wu Lei Lou Yusong Zou Michelle Li Zhong-Ru Xie Yajun Yan Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
description	SpCas9 has a NGG PAM requirement that limits its DNA target range. Here the authors present a PAM-expanded SpdCas9 variant SpdNG-LWQT to target NRN PAMs.
format	article
author	Jian Wang Yuxi Teng Ruihua Zhang Yifei Wu Lei Lou Yusong Zou Michelle Li Zhong-Ru Xie Yajun Yan
author_facet	Jian Wang Yuxi Teng Ruihua Zhang Yifei Wu Lei Lou Yusong Zou Michelle Li Zhong-Ru Xie Yajun Yan
author_sort	Jian Wang
title	Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
title_short	Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
title_full	Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
title_fullStr	Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
title_full_unstemmed	Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor
title_sort	engineering a pam-flexible spdcas9 variant as a universal gene repressor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3a51c52620b74b36aec06b2ae3c5ca95
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Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

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