Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation

G Ciofani1, L Ricotti1, S Danti2,3, S Moscato4, C Nesti2, D D&rsquo;Alessandro2,4, D Dinucci5, F Chiellini5, A Pietrabissa3, M Petrini2,3, A Menciassi1,61Scuola Superiore Sant&rsquo;Anna, Pisa, Italy; 2CUCCS-RRMR, Center for the Clinical Use of Stem Cells &ndash; Regional Net...

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Autores principales:	G Ciofani, L Ricotti, S Danti, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3a57146498d34aba8a686825af0e557b
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spelling	oai:doaj.org-article:3a57146498d34aba8a686825af0e557b2021-12-02T01:16:06ZInvestigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation1176-91141178-2013https://doaj.org/article/3a57146498d34aba8a686825af0e557b2010-04-01T00:00:00Zhttp://www.dovepress.com/investigation-of-interactions-between-poly-l-lysine-coated-boron-nitri-a4233https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013G Ciofani1, L Ricotti1, S Danti2,3, S Moscato4, C Nesti2, D D&rsquo;Alessandro2,4, D Dinucci5, F Chiellini5, A Pietrabissa3, M Petrini2,3, A Menciassi1,61Scuola Superiore Sant&rsquo;Anna, Pisa, Italy; 2CUCCS-RRMR, Center for the Clinical Use of Stem Cells &ndash; Regional Network of Regenerative Medicine, 3Department of Oncology, Transplants and Advanced Technologies, 4Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Italy; 5Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), UdR INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, San Piero a Grado, Italy; 6Italian Institute of Technology, Genova, ItalyAbstract: Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-L-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription &ndash; polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.Keywords: boron nitride nanotubes, C2C12 cells, cytocompatibility, up-take, differentiation, MyoD, connexin 43 G CiofaniL RicottiS Dantiet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 285-298 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 G Ciofani L Ricotti S Danti et al Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
description	G Ciofani1, L Ricotti1, S Danti2,3, S Moscato4, C Nesti2, D D&rsquo;Alessandro2,4, D Dinucci5, F Chiellini5, A Pietrabissa3, M Petrini2,3, A Menciassi1,61Scuola Superiore Sant&rsquo;Anna, Pisa, Italy; 2CUCCS-RRMR, Center for the Clinical Use of Stem Cells &ndash; Regional Network of Regenerative Medicine, 3Department of Oncology, Transplants and Advanced Technologies, 4Department of Human Morphology and Applied Biology, University of Pisa, Pisa, Italy; 5Laboratory of Bioactive Polymeric Materials for Biomedical and Environmental Applications (BIOlab), UdR INSTM, Department of Chemistry and Industrial Chemistry, University of Pisa, San Piero a Grado, Italy; 6Italian Institute of Technology, Genova, ItalyAbstract: Boron nitride nanotubes (BNNTs) have generated considerable interest within the scientific community by virtue of their unique physical properties, which can be exploited in the biomedical field. In the present in vitro study, we investigated the interactions of poly-L-lysine-coated BNNTs with C2C12 cells, as a model of muscle cells, in terms of cytocompatibility and BNNT internalization. The latter was performed using both confocal and transmission electron microscopy. Finally, we investigated myoblast differentiation in the presence of BNNTs, evaluating the protein synthesis of differentiating cells, myotube formation, and expression of some constitutive myoblastic markers, such as MyoD and Cx43, by reverse transcription &ndash; polymerase chain reaction and Western blot analysis. We demonstrated that BNNTs are highly internalized by C2C12 cells, with neither adversely affecting C2C12 myoblast viability nor significantly interfering with myotube formation.Keywords: boron nitride nanotubes, C2C12 cells, cytocompatibility, up-take, differentiation, MyoD, connexin 43
format	article
author	G Ciofani L Ricotti S Danti et al
author_facet	G Ciofani L Ricotti S Danti et al
author_sort	G Ciofani
title	Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
title_short	Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
title_full	Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
title_fullStr	Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
title_full_unstemmed	Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation
title_sort	investigation of interactions between poly-l-lysine-coated boron nitride nanotubes and c2c12 cells: up-take, cytocompatibility, and differentiation
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/3a57146498d34aba8a686825af0e557b
work_keys_str_mv	AT gciofani investigationofinteractionsbetweenpolyllysinecoatedboronnitridenanotubesandc2c12cellsuptakecytocompatibilityanddifferentiation AT lricotti investigationofinteractionsbetweenpolyllysinecoatedboronnitridenanotubesandc2c12cellsuptakecytocompatibilityanddifferentiation AT sdanti investigationofinteractionsbetweenpolyllysinecoatedboronnitridenanotubesandc2c12cellsuptakecytocompatibilityanddifferentiation AT etal investigationofinteractionsbetweenpolyllysinecoatedboronnitridenanotubesandc2c12cellsuptakecytocompatibilityanddifferentiation
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Investigation of interactions between poly-L-lysine-coated boron nitride nanotubes and C2C12 cells: up-take, cytocompatibility, and differentiation

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