Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort

The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated an...

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Autores principales:	Zizhong Tang, Yihan Qin, Wenhui Chen, Zhiqiao Zhao, Wenjie Lin, Yirong Xiao, Hong Chen, Yuntao Liu, Hui Chen, Tongliang Bu, Qingfeng Li, Yi Cai, Huipeng Yao, Yujun Wan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	chuanxiong phenolic compounds endophytic fungi antioxidant activity antimicrobial activity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/3a6149e606c248a7addff5441823927d
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spelling	oai:doaj.org-article:3a6149e606c248a7addff5441823927d2021-11-17T13:59:25ZDiversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort1664-302X10.3389/fmicb.2021.771000https://doaj.org/article/3a6149e606c248a7addff5441823927d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.771000/fullhttps://doaj.org/toc/1664-302XThe objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. Penicillium oxalicum, Simplicillium sp., and Colletotrichum sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of Penicillium oxalicum and Simplicillium sp. and the n-butanol extract of Colletotrichum sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of Penicillium oxalicum had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 ± 0.006% and 90.11 ± 0.032%, respectively. At the same time, their IC50 values were only 0.18 ± 0.02 mg/mL and 0.04 ± 0.003 mg/mL. The ethyl acetate extract of Penicillium oxalicum showed MIC value of only 0.5 mg/mL against Escherichia coli and Staphylococcus aureus. By liquid chromatography-mass spectrometry (LC-MS), we found that Penicillium oxalicum could produce many high-value polyphenols, such as hesperidin (36.06 μmol/g), ferulic acid (1.17 μmol/g), and alternariol (12.64 μmol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.Zizhong TangYihan QinWenhui ChenZhiqiao ZhaoWenjie LinYirong XiaoHong ChenYuntao LiuHui ChenTongliang BuQingfeng LiYi CaiHuipeng YaoYujun WanFrontiers Media S.A.articlechuanxiongphenolic compoundsendophytic fungiantioxidant activityantimicrobial activityMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chuanxiong phenolic compounds endophytic fungi antioxidant activity antimicrobial activity Microbiology QR1-502
spellingShingle	chuanxiong phenolic compounds endophytic fungi antioxidant activity antimicrobial activity Microbiology QR1-502 Zizhong Tang Yihan Qin Wenhui Chen Zhiqiao Zhao Wenjie Lin Yirong Xiao Hong Chen Yuntao Liu Hui Chen Tongliang Bu Qingfeng Li Yi Cai Huipeng Yao Yujun Wan Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
description	The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. Penicillium oxalicum, Simplicillium sp., and Colletotrichum sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of Penicillium oxalicum and Simplicillium sp. and the n-butanol extract of Colletotrichum sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of Penicillium oxalicum had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 ± 0.006% and 90.11 ± 0.032%, respectively. At the same time, their IC50 values were only 0.18 ± 0.02 mg/mL and 0.04 ± 0.003 mg/mL. The ethyl acetate extract of Penicillium oxalicum showed MIC value of only 0.5 mg/mL against Escherichia coli and Staphylococcus aureus. By liquid chromatography-mass spectrometry (LC-MS), we found that Penicillium oxalicum could produce many high-value polyphenols, such as hesperidin (36.06 μmol/g), ferulic acid (1.17 μmol/g), and alternariol (12.64 μmol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.
format	article
author	Zizhong Tang Yihan Qin Wenhui Chen Zhiqiao Zhao Wenjie Lin Yirong Xiao Hong Chen Yuntao Liu Hui Chen Tongliang Bu Qingfeng Li Yi Cai Huipeng Yao Yujun Wan
author_facet	Zizhong Tang Yihan Qin Wenhui Chen Zhiqiao Zhao Wenjie Lin Yirong Xiao Hong Chen Yuntao Liu Hui Chen Tongliang Bu Qingfeng Li Yi Cai Huipeng Yao Yujun Wan
author_sort	Zizhong Tang
title	Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
title_short	Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
title_full	Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
title_fullStr	Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
title_full_unstemmed	Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort
title_sort	diversity, chemical constituents, and biological activities of endophytic fungi isolated from ligusticum chuanxiong hort
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/3a6149e606c248a7addff5441823927d
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Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort

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