Whole transcriptome profiling of taste bud cells

Abstract Analysis of single-cell RNA-Seq data can provide insights into the specific functions of individual cell types that compose complex tissues. Here, we examined gene expression in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiologically identified...

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Autores principales:	Sunil K. Sukumaran, Brian C. Lewandowski, Yumei Qin, Ramana Kotha, Alexander A. Bachmanov, Robert F. Margolskee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a624e5a9e5546a8bc99e886b1acbef3
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spelling	oai:doaj.org-article:3a624e5a9e5546a8bc99e886b1acbef32021-12-02T15:05:49ZWhole transcriptome profiling of taste bud cells10.1038/s41598-017-07746-z2045-2322https://doaj.org/article/3a624e5a9e5546a8bc99e886b1acbef32017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07746-zhttps://doaj.org/toc/2045-2322Abstract Analysis of single-cell RNA-Seq data can provide insights into the specific functions of individual cell types that compose complex tissues. Here, we examined gene expression in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiologically identified type III cells. Our RNA-Seq libraries met high quality control standards and accurately captured differential expression of marker genes for type II (e.g. the Tas1r genes, Plcb2, Trpm5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells. Bioinformatics analysis showed that genes regulating responses to stimuli were up-regulated in type II cells, while pathways related to neuronal function were up-regulated in type III cells. We also identified highly expressed genes and pathways associated with chemotaxis and axon guidance, providing new insights into the mechanisms underlying integration of new taste cells into the taste bud. We validated our results by immunohistochemically confirming expression of selected genes encoding synaptic (Cplx2 and Pclo) and semaphorin signalling pathway (Crmp2, PlexinB1, Fes and Sema4a) components. The approach described here could provide a comprehensive map of gene expression for all taste cell subpopulations and will be particularly relevant for cell types in taste buds and other tissues that can be identified only by physiological methods.Sunil K. SukumaranBrian C. LewandowskiYumei QinRamana KothaAlexander A. BachmanovRobert F. MargolskeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sunil K. Sukumaran Brian C. Lewandowski Yumei Qin Ramana Kotha Alexander A. Bachmanov Robert F. Margolskee Whole transcriptome profiling of taste bud cells
description	Abstract Analysis of single-cell RNA-Seq data can provide insights into the specific functions of individual cell types that compose complex tissues. Here, we examined gene expression in two distinct subpopulations of mouse taste cells: Tas1r3-expressing type II cells and physiologically identified type III cells. Our RNA-Seq libraries met high quality control standards and accurately captured differential expression of marker genes for type II (e.g. the Tas1r genes, Plcb2, Trpm5) and type III (e.g. Pkd2l1, Ncam, Snap25) taste cells. Bioinformatics analysis showed that genes regulating responses to stimuli were up-regulated in type II cells, while pathways related to neuronal function were up-regulated in type III cells. We also identified highly expressed genes and pathways associated with chemotaxis and axon guidance, providing new insights into the mechanisms underlying integration of new taste cells into the taste bud. We validated our results by immunohistochemically confirming expression of selected genes encoding synaptic (Cplx2 and Pclo) and semaphorin signalling pathway (Crmp2, PlexinB1, Fes and Sema4a) components. The approach described here could provide a comprehensive map of gene expression for all taste cell subpopulations and will be particularly relevant for cell types in taste buds and other tissues that can be identified only by physiological methods.
format	article
author	Sunil K. Sukumaran Brian C. Lewandowski Yumei Qin Ramana Kotha Alexander A. Bachmanov Robert F. Margolskee
author_facet	Sunil K. Sukumaran Brian C. Lewandowski Yumei Qin Ramana Kotha Alexander A. Bachmanov Robert F. Margolskee
author_sort	Sunil K. Sukumaran
title	Whole transcriptome profiling of taste bud cells
title_short	Whole transcriptome profiling of taste bud cells
title_full	Whole transcriptome profiling of taste bud cells
title_fullStr	Whole transcriptome profiling of taste bud cells
title_full_unstemmed	Whole transcriptome profiling of taste bud cells
title_sort	whole transcriptome profiling of taste bud cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3a624e5a9e5546a8bc99e886b1acbef3
work_keys_str_mv	AT sunilksukumaran wholetranscriptomeprofilingoftastebudcells AT brianclewandowski wholetranscriptomeprofilingoftastebudcells AT yumeiqin wholetranscriptomeprofilingoftastebudcells AT ramanakotha wholetranscriptomeprofilingoftastebudcells AT alexanderabachmanov wholetranscriptomeprofilingoftastebudcells AT robertfmargolskee wholetranscriptomeprofilingoftastebudcells
_version_	1718388697510969344

Whole transcriptome profiling of taste bud cells

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