Construction of the hit-deficient mutant strain of Streptococcus mutans ATCC25175

Construction of the hit-deficient mutant strain of Streptococcus mutans ATCC25175

Objective To construct a hit-deficient mutant strain of S. mutans ATCC25175 and verify its cell cycle regulatory function. Method Genomic DNA was extracted from S. mutans ATCC25175 strains, and then the upstream and downstream DNA fragments of the hit gene were cloned into the pFW5 vector (spectinom...

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Bibliographic Details
Main Authors: LAI Yangfan, WANG Peng, QIAO Li, LIU Zhongjing, YE Zhaoyang, LIANG Yan
Format: article
Language:ZH
Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 2021
Subjects:
streptococcus mutans ，
hit gene，
pfw5 vector，
natural genetic transformation，
homologous recombination，
hit-deficient mutant strain，
cell growth，
cell-cycle regulation，
Medicine
R
Online Access:https://doaj.org/article/3a63e10fdf6e403aa69bbd25c6601634
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