Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets

Objectives. To evaluate the microhardness of tooth enamel remineralized with enamel matrix protein solution as well as the shear bond strength of orthodontic brackets bonded to this surface. Materials and Methods. In total, 24 human premolars were selected and divided into 3 experimental groups (n =...

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Autores principales:	Guilherme Genovez-Júnior, Sandrine Bittencourt Berger, Lucineide Lima dos Santos, Eloisa Aparecida Carlesse Paloco, Murilo Baena Lopes, Débora Fernandes Giuliangeli, Júlia Graciela Monteiro dos Santos, Ricardo Danil Guiraldo
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/3a68e69eedd94adf8fcbed0e1201d72c
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spelling	oai:doaj.org-article:3a68e69eedd94adf8fcbed0e1201d72c2021-11-08T02:35:35ZEffect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets1687-873610.1155/2021/7025910https://doaj.org/article/3a68e69eedd94adf8fcbed0e1201d72c2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/7025910https://doaj.org/toc/1687-8736Objectives. To evaluate the microhardness of tooth enamel remineralized with enamel matrix protein solution as well as the shear bond strength of orthodontic brackets bonded to this surface. Materials and Methods. In total, 24 human premolars were selected and divided into 3 experimental groups (n = 8): SE—sound enamel, DE—demineralized enamel, and TE—demineralized enamel treated with amelogenin solution. Samples from DE and TE groups were subjected to pH cycling to induce initial artificial caries lesion. TE group was treated with amelogenin solution. Samples were placed in artificial saliva for 7 days. Knoop microhardness was measured before any intervention (T0), after pH cycling (T1) and after amelogenin solution treatment application (T2). Twenty-four hours after ceramic orthodontic brackets were bonded, samples were subjected to shear test in a universal testing machine. Microhardness and shear measurement distributions were subjected to Kolmogorov–Smirnov normality test, which was followed by parametric tests (α = 0.05): 2-way analysis of variance (factors: enamel condition × treatment) and Tukey posttest for all three groups (SE, DE, and TE) in T0 and T2 for microhardness; analysis of variance and Tukey’s test, for shear bond strength test. Results. Means recorded for Knoop microhardness in T2, for the SE (366.7 KHN) and TE (342.8 KHN) groups, were significantly higher than those recorded for the DE group (263.5 KHN). The shear bond strength of the SE (15.44 MPa) and TE (14.84 MPa) groups statistically differed from that of the DE group (11.95 MPa). Conclusion. In vitro demineralized enamel treatment with amelogenin solution was capable of taking samples’ hardness back to levels similar to those observed for sound enamel. The shear bond strength on the enamel subjected to this treatment was similar to that observed for healthy enamel and higher than that observed for demineralized enamel.Guilherme Genovez-JúniorSandrine Bittencourt BergerLucineide Lima dos SantosEloisa Aparecida Carlesse PalocoMurilo Baena LopesDébora Fernandes GiuliangeliJúlia Graciela Monteiro dos SantosRicardo Danil GuiraldoHindawi LimitedarticleDentistryRK1-715ENInternational Journal of Dentistry, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dentistry RK1-715
spellingShingle	Dentistry RK1-715 Guilherme Genovez-Júnior Sandrine Bittencourt Berger Lucineide Lima dos Santos Eloisa Aparecida Carlesse Paloco Murilo Baena Lopes Débora Fernandes Giuliangeli Júlia Graciela Monteiro dos Santos Ricardo Danil Guiraldo Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
description	Objectives. To evaluate the microhardness of tooth enamel remineralized with enamel matrix protein solution as well as the shear bond strength of orthodontic brackets bonded to this surface. Materials and Methods. In total, 24 human premolars were selected and divided into 3 experimental groups (n = 8): SE—sound enamel, DE—demineralized enamel, and TE—demineralized enamel treated with amelogenin solution. Samples from DE and TE groups were subjected to pH cycling to induce initial artificial caries lesion. TE group was treated with amelogenin solution. Samples were placed in artificial saliva for 7 days. Knoop microhardness was measured before any intervention (T0), after pH cycling (T1) and after amelogenin solution treatment application (T2). Twenty-four hours after ceramic orthodontic brackets were bonded, samples were subjected to shear test in a universal testing machine. Microhardness and shear measurement distributions were subjected to Kolmogorov–Smirnov normality test, which was followed by parametric tests (α = 0.05): 2-way analysis of variance (factors: enamel condition × treatment) and Tukey posttest for all three groups (SE, DE, and TE) in T0 and T2 for microhardness; analysis of variance and Tukey’s test, for shear bond strength test. Results. Means recorded for Knoop microhardness in T2, for the SE (366.7 KHN) and TE (342.8 KHN) groups, were significantly higher than those recorded for the DE group (263.5 KHN). The shear bond strength of the SE (15.44 MPa) and TE (14.84 MPa) groups statistically differed from that of the DE group (11.95 MPa). Conclusion. In vitro demineralized enamel treatment with amelogenin solution was capable of taking samples’ hardness back to levels similar to those observed for sound enamel. The shear bond strength on the enamel subjected to this treatment was similar to that observed for healthy enamel and higher than that observed for demineralized enamel.
format	article
author	Guilherme Genovez-Júnior Sandrine Bittencourt Berger Lucineide Lima dos Santos Eloisa Aparecida Carlesse Paloco Murilo Baena Lopes Débora Fernandes Giuliangeli Júlia Graciela Monteiro dos Santos Ricardo Danil Guiraldo
author_facet	Guilherme Genovez-Júnior Sandrine Bittencourt Berger Lucineide Lima dos Santos Eloisa Aparecida Carlesse Paloco Murilo Baena Lopes Débora Fernandes Giuliangeli Júlia Graciela Monteiro dos Santos Ricardo Danil Guiraldo
author_sort	Guilherme Genovez-Júnior
title	Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
title_short	Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
title_full	Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
title_fullStr	Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
title_full_unstemmed	Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets
title_sort	effect of amelogenin solution in the microhardness of remineralized enamel and shear bond strength of orthodontic brackets
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/3a68e69eedd94adf8fcbed0e1201d72c
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Effect of Amelogenin Solution in the Microhardness of Remineralized Enamel and Shear Bond Strength of Orthodontic Brackets

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