Obesity alters the collagen organization and mechanical properties of murine cartilage

Abstract Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on...

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Autores principales:	Amber T. Collins, Guoli Hu, Hunter Newman, Michael H. Reinsvold, Monique R. Goldsmith, John N. Twomey-Kozak, Holly A. Leddy, Deepika Sharma, Leyao Shen, Louis E. DeFrate, Courtney M. Karner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a6956c4a3614608acf2c34be56a4605
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spelling	oai:doaj.org-article:3a6956c4a3614608acf2c34be56a46052021-12-02T14:12:43ZObesity alters the collagen organization and mechanical properties of murine cartilage10.1038/s41598-020-80599-12045-2322https://doaj.org/article/3a6956c4a3614608acf2c34be56a46052021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80599-1https://doaj.org/toc/2045-2322Abstract Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.Amber T. CollinsGuoli HuHunter NewmanMichael H. ReinsvoldMonique R. GoldsmithJohn N. Twomey-KozakHolly A. LeddyDeepika SharmaLeyao ShenLouis E. DeFrateCourtney M. KarnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amber T. Collins Guoli Hu Hunter Newman Michael H. Reinsvold Monique R. Goldsmith John N. Twomey-Kozak Holly A. Leddy Deepika Sharma Leyao Shen Louis E. DeFrate Courtney M. Karner Obesity alters the collagen organization and mechanical properties of murine cartilage
description	Abstract Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.
format	article
author	Amber T. Collins Guoli Hu Hunter Newman Michael H. Reinsvold Monique R. Goldsmith John N. Twomey-Kozak Holly A. Leddy Deepika Sharma Leyao Shen Louis E. DeFrate Courtney M. Karner
author_facet	Amber T. Collins Guoli Hu Hunter Newman Michael H. Reinsvold Monique R. Goldsmith John N. Twomey-Kozak Holly A. Leddy Deepika Sharma Leyao Shen Louis E. DeFrate Courtney M. Karner
author_sort	Amber T. Collins
title	Obesity alters the collagen organization and mechanical properties of murine cartilage
title_short	Obesity alters the collagen organization and mechanical properties of murine cartilage
title_full	Obesity alters the collagen organization and mechanical properties of murine cartilage
title_fullStr	Obesity alters the collagen organization and mechanical properties of murine cartilage
title_full_unstemmed	Obesity alters the collagen organization and mechanical properties of murine cartilage
title_sort	obesity alters the collagen organization and mechanical properties of murine cartilage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3a6956c4a3614608acf2c34be56a4605
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Obesity alters the collagen organization and mechanical properties of murine cartilage

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