In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.

A chemical inhibition model of inflammation is proposed by semi-continuous monitoring the density of toll-like receptor 1 (TLR1) expressed on mammalian cells following bacterial infection to investigate an in vivo-mimicked drug screening system. The inflammation was induced by adding bacterial lysat...

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Autores principales:	Jin-Woo Jeon, Un-Hwan Ha, Se-Hwan Paek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a6e3369db3042cc983bfebb5de0e5c4
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spelling	oai:doaj.org-article:3a6e3369db3042cc983bfebb5de0e5c42021-11-25T06:04:11ZIn vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.1932-620310.1371/journal.pone.0105212https://doaj.org/article/3a6e3369db3042cc983bfebb5de0e5c42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25136864/?tool=EBIhttps://doaj.org/toc/1932-6203A chemical inhibition model of inflammation is proposed by semi-continuous monitoring the density of toll-like receptor 1 (TLR1) expressed on mammalian cells following bacterial infection to investigate an in vivo-mimicked drug screening system. The inflammation was induced by adding bacterial lysate (e.g., Pseudomonas aeruginosa) to a mammalian cell culture (e.g., A549 cell line). The TLR1 density on the same cells was immunochemically monitored up to three cycles under optimized cyclic bacterial stimulation-and-restoration conditions. The assay was carried out by adopting a cell-compatible immunoanalytical procedure and signal generation method. Signal intensity relative to the background control obtained without stimulation was employed to plot the standard curve for inflammation. To suppress the inflammatory response, sodium salicylate, which inhibits nuclear factor-κB activity, was used to prepare the standard curve for anti-inflammation. Such measurement of differential TLR densities was used as a biosensing approach discriminating the anti-inflammatory substance from the non-effector, which was simulated by using caffeic acid phenethyl ester and acetaminophen as the two components, respectively. As the same cells exposed to repetitive bacterial stimulation were semi-continuously monitored, the efficacy and toxicity of the inhibitors may further be determined regarding persistency against time. Therefore, this semi-continuous biosensing model could be appropriate as a substitute for animal-based experimentation during drug screening prior to pre-clinical tests.Jin-Woo JeonUn-Hwan HaSe-Hwan PaekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105212 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jin-Woo Jeon Un-Hwan Ha Se-Hwan Paek In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
description	A chemical inhibition model of inflammation is proposed by semi-continuous monitoring the density of toll-like receptor 1 (TLR1) expressed on mammalian cells following bacterial infection to investigate an in vivo-mimicked drug screening system. The inflammation was induced by adding bacterial lysate (e.g., Pseudomonas aeruginosa) to a mammalian cell culture (e.g., A549 cell line). The TLR1 density on the same cells was immunochemically monitored up to three cycles under optimized cyclic bacterial stimulation-and-restoration conditions. The assay was carried out by adopting a cell-compatible immunoanalytical procedure and signal generation method. Signal intensity relative to the background control obtained without stimulation was employed to plot the standard curve for inflammation. To suppress the inflammatory response, sodium salicylate, which inhibits nuclear factor-κB activity, was used to prepare the standard curve for anti-inflammation. Such measurement of differential TLR densities was used as a biosensing approach discriminating the anti-inflammatory substance from the non-effector, which was simulated by using caffeic acid phenethyl ester and acetaminophen as the two components, respectively. As the same cells exposed to repetitive bacterial stimulation were semi-continuously monitored, the efficacy and toxicity of the inhibitors may further be determined regarding persistency against time. Therefore, this semi-continuous biosensing model could be appropriate as a substitute for animal-based experimentation during drug screening prior to pre-clinical tests.
format	article
author	Jin-Woo Jeon Un-Hwan Ha Se-Hwan Paek
author_facet	Jin-Woo Jeon Un-Hwan Ha Se-Hwan Paek
author_sort	Jin-Woo Jeon
title	In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
title_short	In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
title_full	In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
title_fullStr	In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
title_full_unstemmed	In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
title_sort	in vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3a6e3369db3042cc983bfebb5de0e5c4
work_keys_str_mv	AT jinwoojeon invitroinflammationinhibitionmodelbasedonsemicontinuoustolllikereceptorbiosensing AT unhwanha invitroinflammationinhibitionmodelbasedonsemicontinuoustolllikereceptorbiosensing AT sehwanpaek invitroinflammationinhibitionmodelbasedonsemicontinuoustolllikereceptorbiosensing
_version_	1718414215611416576

In vitro inflammation inhibition model based on semi-continuous toll-like receptor biosensing.

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