Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy

Gestational hypertension is a common disease in clinical practice, which does great harm to the mother and infant. The purpose of this study was to investigate the relationship between 25-hydroxyvitamin D, sFlt-1, and PLGF and hypertensive disorder complicating pregnancy. Specimen preparation: after...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Wang, Ting Du, Xiaoqi Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine (General) R5-920 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/3a6e6bbe73c1468cbf43d2b23c0fbe32
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3a6e6bbe73c1468cbf43d2b23c0fbe32
record_format	dspace
spelling	oai:doaj.org-article:3a6e6bbe73c1468cbf43d2b23c0fbe322021-11-15T01:19:51ZCorrelation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy2040-230910.1155/2021/9371953https://doaj.org/article/3a6e6bbe73c1468cbf43d2b23c0fbe322021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9371953https://doaj.org/toc/2040-2309Gestational hypertension is a common disease in clinical practice, which does great harm to the mother and infant. The purpose of this study was to investigate the relationship between 25-hydroxyvitamin D, sFlt-1, and PLGF and hypertensive disorder complicating pregnancy. Specimen preparation: after delivery or placental caesarean section, in order to avoid calcification and necrosis in the middle of the placenta, an area of about 1.5 cm × 1.5 cm × 1.5 cm should be separated immediately. After dehydration, use a Citadel 2000 dryer to dry it and place it in a block of saline for xylene immunohistochemical staining. Statistical processing was performed according to the proportion of positive cells in each part and the depth of staining. Placental tissue collection and treatment: within 20 minutes after the delivery of the placenta, two pieces of the placental tissue (about 1.0 cm × 1.0 cm × 1.0 cm) were taken from the central zone of the placental maternal surface without obvious bleeding and calcification. They were rinsed repeatedly in normal saline, fixed in 10% neutral formaldehyde solution for 24 hours, dehydrated using an automatic dehydrator, and embedded in paraffin for detection. Before the study, 20 ml of distilled water was added to the sample to stand for 20 minutes; the Cobas E610 immunoanalyzer was turned on, and sFlt-1 and PLGF (placental growth factor) were selected. The serum 25-hydroxyvitamin D level of pregnant women was detected in the fasting state at 24–28 weeks of gestation, and the best collection time was 8 : 00–11 : 00 in the morning. 5 ml of the whole blood sample without anticoagulant was collected and stored at 0–4°C in a cold storage and dark environment. The serum was obtained by high-speed centrifugation within 24 hours after collection. The method is suitable for the quantitative determination of 25-OH-vitamin D in human serum. In the hypertensive pregnancy group, the level of 25-hydroxyvitamin D was 18.44 ± 3.48 ng/ml, and the sFlt-1/PLGF level was highest at 0–5 weeks followed by 5–10 weeks, 10–15 weeks, and 15–20 weeks. This study provides new ideas and experimental clues for the prevention and treatment of pre-eclampsia.Wei WangTing DuXiaoqi JiangHindawi LimitedarticleMedicine (General)R5-920Medical technologyR855-855.5ENJournal of Healthcare Engineering, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920 Medical technology R855-855.5
spellingShingle	Medicine (General) R5-920 Medical technology R855-855.5 Wei Wang Ting Du Xiaoqi Jiang Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
description	Gestational hypertension is a common disease in clinical practice, which does great harm to the mother and infant. The purpose of this study was to investigate the relationship between 25-hydroxyvitamin D, sFlt-1, and PLGF and hypertensive disorder complicating pregnancy. Specimen preparation: after delivery or placental caesarean section, in order to avoid calcification and necrosis in the middle of the placenta, an area of about 1.5 cm × 1.5 cm × 1.5 cm should be separated immediately. After dehydration, use a Citadel 2000 dryer to dry it and place it in a block of saline for xylene immunohistochemical staining. Statistical processing was performed according to the proportion of positive cells in each part and the depth of staining. Placental tissue collection and treatment: within 20 minutes after the delivery of the placenta, two pieces of the placental tissue (about 1.0 cm × 1.0 cm × 1.0 cm) were taken from the central zone of the placental maternal surface without obvious bleeding and calcification. They were rinsed repeatedly in normal saline, fixed in 10% neutral formaldehyde solution for 24 hours, dehydrated using an automatic dehydrator, and embedded in paraffin for detection. Before the study, 20 ml of distilled water was added to the sample to stand for 20 minutes; the Cobas E610 immunoanalyzer was turned on, and sFlt-1 and PLGF (placental growth factor) were selected. The serum 25-hydroxyvitamin D level of pregnant women was detected in the fasting state at 24–28 weeks of gestation, and the best collection time was 8 : 00–11 : 00 in the morning. 5 ml of the whole blood sample without anticoagulant was collected and stored at 0–4°C in a cold storage and dark environment. The serum was obtained by high-speed centrifugation within 24 hours after collection. The method is suitable for the quantitative determination of 25-OH-vitamin D in human serum. In the hypertensive pregnancy group, the level of 25-hydroxyvitamin D was 18.44 ± 3.48 ng/ml, and the sFlt-1/PLGF level was highest at 0–5 weeks followed by 5–10 weeks, 10–15 weeks, and 15–20 weeks. This study provides new ideas and experimental clues for the prevention and treatment of pre-eclampsia.
format	article
author	Wei Wang Ting Du Xiaoqi Jiang
author_facet	Wei Wang Ting Du Xiaoqi Jiang
author_sort	Wei Wang
title	Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
title_short	Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
title_full	Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
title_fullStr	Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
title_full_unstemmed	Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy
title_sort	correlation between 25-hydroxyvitamin d, sflt-1, plgf, and hypertension in pregnancy
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/3a6e6bbe73c1468cbf43d2b23c0fbe32
work_keys_str_mv	AT weiwang correlationbetween25hydroxyvitamindsflt1plgfandhypertensioninpregnancy AT tingdu correlationbetween25hydroxyvitamindsflt1plgfandhypertensioninpregnancy AT xiaoqijiang correlationbetween25hydroxyvitamindsflt1plgfandhypertensioninpregnancy
_version_	1718428916532641792

Correlation between 25-Hydroxyvitamin D, sFlt-1, PLGF, and Hypertension in Pregnancy

Ejemplares similares