Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards

The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases. Short chain fatty acids (SCFAs) are key metabolites produced by...

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Autores principales:	Shikha Saha, Priscilla Day-Walsh, Emad Shehata, Paul Anthony Kroon
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	gut microbiota kidney diabetes neurodegenerative cardiovascular plasma Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/3a7312b4c5444af5b85d2a5ce2096c68
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spelling	oai:doaj.org-article:3a7312b4c5444af5b85d2a5ce2096c682021-11-11T18:27:30ZDevelopment and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards10.3390/molecules262164441420-3049https://doaj.org/article/3a7312b4c5444af5b85d2a5ce2096c682021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6444https://doaj.org/toc/1420-3049The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases. Short chain fatty acids (SCFAs) are key metabolites produced by the gut microbiota from the fermentation of dietary fibre. Here we present a novel, sensitive, and direct LC-MS/MS technique using isotopically labelled internal standards without derivatisation for the analysis of SCFAs in different biological matrices. The technique has significant advantages over the current widely used techniques based on sample derivatization and GC-MS analysis, including fast and simple sample preparation and short LC runtime (10 min). The technique is specific and sensitive for the quantification of acetate, butyrate, isobutyrate, isovalerate, lactate, propionate and valerate. The limits of detection were all 0.001 mM except for acetate which was 0.003 mM. The calibration curves for all the analytes were linear with correlation coefficients r<sup>2</sup> > 0.998. The intra- and inter-day precisions in three levels of known concentrations were <12% and <20%, respectively. The quantification accuracy ranged from 92% to 120%. The technique reported here offers a valuable analytical tool for use in studies of SCFA production in the gut and their distribution to host tissues.Shikha SahaPriscilla Day-WalshEmad ShehataPaul Anthony KroonMDPI AGarticlegut microbiotakidneydiabetesneurodegenerativecardiovascularplasmaOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6444, p 6444 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiota kidney diabetes neurodegenerative cardiovascular plasma Organic chemistry QD241-441
spellingShingle	gut microbiota kidney diabetes neurodegenerative cardiovascular plasma Organic chemistry QD241-441 Shikha Saha Priscilla Day-Walsh Emad Shehata Paul Anthony Kroon Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
description	The gut microbiota is critical to the maintenance of physiological homeostasis and as such is implicated in a range of diseases such as colon cancer, ulcerative colitis, diabetes, cardiovascular diseases, and neurodegenerative diseases. Short chain fatty acids (SCFAs) are key metabolites produced by the gut microbiota from the fermentation of dietary fibre. Here we present a novel, sensitive, and direct LC-MS/MS technique using isotopically labelled internal standards without derivatisation for the analysis of SCFAs in different biological matrices. The technique has significant advantages over the current widely used techniques based on sample derivatization and GC-MS analysis, including fast and simple sample preparation and short LC runtime (10 min). The technique is specific and sensitive for the quantification of acetate, butyrate, isobutyrate, isovalerate, lactate, propionate and valerate. The limits of detection were all 0.001 mM except for acetate which was 0.003 mM. The calibration curves for all the analytes were linear with correlation coefficients r<sup>2</sup> > 0.998. The intra- and inter-day precisions in three levels of known concentrations were <12% and <20%, respectively. The quantification accuracy ranged from 92% to 120%. The technique reported here offers a valuable analytical tool for use in studies of SCFA production in the gut and their distribution to host tissues.
format	article
author	Shikha Saha Priscilla Day-Walsh Emad Shehata Paul Anthony Kroon
author_facet	Shikha Saha Priscilla Day-Walsh Emad Shehata Paul Anthony Kroon
author_sort	Shikha Saha
title	Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
title_short	Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
title_full	Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
title_fullStr	Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
title_full_unstemmed	Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards
title_sort	development and validation of a lc-ms/ms technique for the analysis of short chain fatty acids in tissues and biological fluids without derivatisation using isotope labelled internal standards
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3a7312b4c5444af5b85d2a5ce2096c68
work_keys_str_mv	AT shikhasaha developmentandvalidationofalcmsmstechniquefortheanalysisofshortchainfattyacidsintissuesandbiologicalfluidswithoutderivatisationusingisotopelabelledinternalstandards AT priscilladaywalsh developmentandvalidationofalcmsmstechniquefortheanalysisofshortchainfattyacidsintissuesandbiologicalfluidswithoutderivatisationusingisotopelabelledinternalstandards AT emadshehata developmentandvalidationofalcmsmstechniquefortheanalysisofshortchainfattyacidsintissuesandbiologicalfluidswithoutderivatisationusingisotopelabelledinternalstandards AT paulanthonykroon developmentandvalidationofalcmsmstechniquefortheanalysisofshortchainfattyacidsintissuesandbiologicalfluidswithoutderivatisationusingisotopelabelledinternalstandards
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Development and Validation of a LC-MS/MS Technique for the Analysis of Short Chain Fatty Acids in Tissues and Biological Fluids without Derivatisation Using Isotope Labelled Internal Standards

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