Discovery of circulating proteins associated to knee radiographic osteoarthritis

Abstract Currently there are no sufficiently sensitive biomarkers able to reflect changes in joint remodelling during osteoarthritis (OA). In this work, we took an affinity proteomic approach to profile serum samples for proteins that could serve as indicators for the diagnosis of radiographic knee...

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Autores principales:	Lucía Lourido, Burcu Ayoglu, Juan Fernández-Tajes, Natividad Oreiro, Frauke Henjes, Cecilia Hellström, Jochen M. Schwenk, Cristina Ruiz-Romero, Peter Nilsson, Francisco J. Blanco
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a7ea01609d049a6b58e2d5dd893dd3f
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spelling	oai:doaj.org-article:3a7ea01609d049a6b58e2d5dd893dd3f2021-12-02T11:51:02ZDiscovery of circulating proteins associated to knee radiographic osteoarthritis10.1038/s41598-017-00195-82045-2322https://doaj.org/article/3a7ea01609d049a6b58e2d5dd893dd3f2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00195-8https://doaj.org/toc/2045-2322Abstract Currently there are no sufficiently sensitive biomarkers able to reflect changes in joint remodelling during osteoarthritis (OA). In this work, we took an affinity proteomic approach to profile serum samples for proteins that could serve as indicators for the diagnosis of radiographic knee OA. Antibody suspension bead arrays were applied to analyze serum samples from patients with OA (n = 273), control subjects (n = 76) and patients with rheumatoid arthritis (RA, n = 244). For verification, a focused bead array was built and applied to an independent set of serum samples from patients with OA (n = 188), control individuals (n = 83) and RA (n = 168) patients. A linear regression analysis adjusting for sex, age and body mass index (BMI) revealed that three proteins were significantly elevated (P < 0.05) in serum from OA patients compared to controls: C3, ITIH1 and S100A6. A panel consisting of these three proteins had an area under the curve of 0.82 for the classification of OA and control samples. Moreover, C3 and ITIH1 levels were also found to be significantly elevated (P < 0.05) in OA patients compared to RA patients. Upon validation in additional study sets, the alterations of these three candidate serum biomarker proteins could support the diagnosis of radiographic knee OA.Lucía LouridoBurcu AyogluJuan Fernández-TajesNatividad OreiroFrauke HenjesCecilia HellströmJochen M. SchwenkCristina Ruiz-RomeroPeter NilssonFrancisco J. BlancoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucía Lourido Burcu Ayoglu Juan Fernández-Tajes Natividad Oreiro Frauke Henjes Cecilia Hellström Jochen M. Schwenk Cristina Ruiz-Romero Peter Nilsson Francisco J. Blanco Discovery of circulating proteins associated to knee radiographic osteoarthritis
description	Abstract Currently there are no sufficiently sensitive biomarkers able to reflect changes in joint remodelling during osteoarthritis (OA). In this work, we took an affinity proteomic approach to profile serum samples for proteins that could serve as indicators for the diagnosis of radiographic knee OA. Antibody suspension bead arrays were applied to analyze serum samples from patients with OA (n = 273), control subjects (n = 76) and patients with rheumatoid arthritis (RA, n = 244). For verification, a focused bead array was built and applied to an independent set of serum samples from patients with OA (n = 188), control individuals (n = 83) and RA (n = 168) patients. A linear regression analysis adjusting for sex, age and body mass index (BMI) revealed that three proteins were significantly elevated (P < 0.05) in serum from OA patients compared to controls: C3, ITIH1 and S100A6. A panel consisting of these three proteins had an area under the curve of 0.82 for the classification of OA and control samples. Moreover, C3 and ITIH1 levels were also found to be significantly elevated (P < 0.05) in OA patients compared to RA patients. Upon validation in additional study sets, the alterations of these three candidate serum biomarker proteins could support the diagnosis of radiographic knee OA.
format	article
author	Lucía Lourido Burcu Ayoglu Juan Fernández-Tajes Natividad Oreiro Frauke Henjes Cecilia Hellström Jochen M. Schwenk Cristina Ruiz-Romero Peter Nilsson Francisco J. Blanco
author_facet	Lucía Lourido Burcu Ayoglu Juan Fernández-Tajes Natividad Oreiro Frauke Henjes Cecilia Hellström Jochen M. Schwenk Cristina Ruiz-Romero Peter Nilsson Francisco J. Blanco
author_sort	Lucía Lourido
title	Discovery of circulating proteins associated to knee radiographic osteoarthritis
title_short	Discovery of circulating proteins associated to knee radiographic osteoarthritis
title_full	Discovery of circulating proteins associated to knee radiographic osteoarthritis
title_fullStr	Discovery of circulating proteins associated to knee radiographic osteoarthritis
title_full_unstemmed	Discovery of circulating proteins associated to knee radiographic osteoarthritis
title_sort	discovery of circulating proteins associated to knee radiographic osteoarthritis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3a7ea01609d049a6b58e2d5dd893dd3f
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Discovery of circulating proteins associated to knee radiographic osteoarthritis

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