Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

<h4>Objectives</h4>The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.<h4>Design</h4>A case-control study included 41 gingival biopsies obtain...

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Main Authors:	Francisco Mesa, Francisco O'Valle, Manfredi Rizzo, Francesco Cappello, Nikos Donos, Mohamed Parkar, Navidah Chaudhary, Francesco Carini, Ricardo Muñoz, Luigi Nibali
Format:	article
Language:	EN
Published:	Public Library of Science (PLoS) 2014
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/3a89744b747c445eb99ddac60d901d7c
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spelling	oai:doaj.org-article:3a89744b747c445eb99ddac60d901d7c2021-11-18T08:32:41ZAssociation between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.1932-620310.1371/journal.pone.0087023https://doaj.org/article/3a89744b747c445eb99ddac60d901d7c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24551049/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objectives</h4>The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.<h4>Design</h4>A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.<h4>Results</h4>The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).<h4>Conclusions</h4>COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.Francisco MesaFrancisco O'ValleManfredi RizzoFrancesco CappelloNikos DonosMohamed ParkarNavidah ChaudharyFrancesco CariniRicardo MuñozLuigi NibaliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87023 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco Mesa Francisco O'Valle Manfredi Rizzo Francesco Cappello Nikos Donos Mohamed Parkar Navidah Chaudhary Francesco Carini Ricardo Muñoz Luigi Nibali Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
description	<h4>Objectives</h4>The aim of this pilot study was to investigate associations between IL-6 and COX-2 expression in gingival biopsies and both clinical diagnosis and genotypes in the IL-6 and COX-2 genes.<h4>Design</h4>A case-control study included 41 gingival biopsies obtained from Caucasian patients grouped according to clinical diagnosis of gingival health (n = 10), gingivitis (n = 15) or chronic periodontitis (n = 16). Immunohistochemistry analyses were performed to determine COX-2 expression in lamina propria, IL-6 expression in lamina propria and gingival epithelium and level of inflammatory cell infiltrate. Individual DNA was extracted and genotyped by real-time PCR for IL6 SNPs rs 2069827 and rs 2069825 and for COX-2 rs 6681231.<h4>Results</h4>The percentage of cellular COX-2 expression was associated with the extent of periodontal disease (Arbes index p = 0.026) and inflammatory infiltrate (p<0.0001). No association was observed between IL6 haplotypes and cells positive to IL-6 or COX-2 in gingival tissues. The COX-2 rs 6681231 was associated with cells positive to IL-6 in the connective tissue (p = 0.032).<h4>Conclusions</h4>COX-2 expression in gingival tissues may be a marker of periodontal disease severity. COX-2 rs 6681231 may be associated with IL-6 local production in gingival tissues.
format	article
author	Francisco Mesa Francisco O'Valle Manfredi Rizzo Francesco Cappello Nikos Donos Mohamed Parkar Navidah Chaudhary Francesco Carini Ricardo Muñoz Luigi Nibali
author_facet	Francisco Mesa Francisco O'Valle Manfredi Rizzo Francesco Cappello Nikos Donos Mohamed Parkar Navidah Chaudhary Francesco Carini Ricardo Muñoz Luigi Nibali
author_sort	Francisco Mesa
title	Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
title_short	Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
title_full	Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
title_fullStr	Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
title_full_unstemmed	Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.
title_sort	association between cox-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. a pilot study.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3a89744b747c445eb99ddac60d901d7c
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Association between COX-2 rs 6681231 genotype and interleukin-6 in periodontal connective tissue. A pilot study.

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