Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection

Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection

Abstract We have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch olig...

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Main Authors: Boon Lim, Jeremy Ratcliff, Dorota A. Nawrot, Yejiong Yu, Harshmeena R. Sanghani, Chia-Chen Hsu, Leon Peto, Simon Evans, Susanne H. Hodgson, Aikaterini Skeva, Maria Adam, Maria Panopoulou, Christos E. Zois, Katy Poncin, Sridhar R. Vasudevan, Siqi Dai, Shuai Ren, Hong Chang, Zhanfeng Cui, Peter Simmonds, Wei E. Huang, Monique I. Andersson
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
Medicine
R
Science
Q
Online Access:https://doaj.org/article/3a8b6fea2a544cd0a2eafc13f941fe38
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https://doaj.org/article/3a8b6fea2a544cd0a2eafc13f941fe38

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