Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)

Abstract Uniconazole (S-(+)-uniconazole), a plant growth retardant, exerts key roles in modulating growth and development and increasing abiotic stress tolerance in plants. However, the underlying mechanisms by which uniconazole regulates drought response remain largely unknown. Here, the effects of...

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Autores principales: Ying Jiang, Yufeng Sun, Dianfeng Zheng, Chengwei Han, Kun Cao, Lei Xu, Shuxia Liu, Yanyong Cao, Naijie Feng
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/3a9378c820824a2da4650b6b0ec23629
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spelling oai:doaj.org-article:3a9378c820824a2da4650b6b0ec236292021-12-02T16:08:08ZPhysiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)10.1038/s41598-021-93820-62045-2322https://doaj.org/article/3a9378c820824a2da4650b6b0ec236292021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93820-6https://doaj.org/toc/2045-2322Abstract Uniconazole (S-(+)-uniconazole), a plant growth retardant, exerts key roles in modulating growth and development and increasing abiotic stress tolerance in plants. However, the underlying mechanisms by which uniconazole regulates drought response remain largely unknown. Here, the effects of exogenous uniconazole on drought tolerance in hemp were studied via physiological and transcriptome analyses of the drought-sensitive industrial hemp cultivar Hanma No. 2 grown under drought stress. Exogenous uniconazole treatment increased hemp tolerance to drought-induced damage by enhancing chlorophyll content and photosynthesis capacity, regulating activities of enzymes involved in carbon and nitrogen metabolism, and altering endogenous hormone levels. Expression of genes associated with porphyrin and chlorophyll metabolism, photosynthesis-antenna proteins, photosynthesis, starch and sucrose metabolism, nitrogen metabolism, and plant hormone signal transduction were significantly regulated by uniconazole compared with that by control (distilled water) under drought stress. Numerous genes were differentially expressed to increase chlorophyll content, enhance photosynthesis, regulate carbon–nitrogen metabolism-related enzyme activities, and alter endogenous hormone levels. Thus, uniconazole regulated physiological and molecular characteristics of photosynthesis, carbon–nitrogen metabolism, and plant hormone signal transduction to enhance drought resistance in industrial hemp.Ying JiangYufeng SunDianfeng ZhengChengwei HanKun CaoLei XuShuxia LiuYanyong CaoNaijie FengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Ying Jiang
Yufeng Sun
Dianfeng Zheng
Chengwei Han
Kun Cao
Lei Xu
Shuxia Liu
Yanyong Cao
Naijie Feng
Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
description Abstract Uniconazole (S-(+)-uniconazole), a plant growth retardant, exerts key roles in modulating growth and development and increasing abiotic stress tolerance in plants. However, the underlying mechanisms by which uniconazole regulates drought response remain largely unknown. Here, the effects of exogenous uniconazole on drought tolerance in hemp were studied via physiological and transcriptome analyses of the drought-sensitive industrial hemp cultivar Hanma No. 2 grown under drought stress. Exogenous uniconazole treatment increased hemp tolerance to drought-induced damage by enhancing chlorophyll content and photosynthesis capacity, regulating activities of enzymes involved in carbon and nitrogen metabolism, and altering endogenous hormone levels. Expression of genes associated with porphyrin and chlorophyll metabolism, photosynthesis-antenna proteins, photosynthesis, starch and sucrose metabolism, nitrogen metabolism, and plant hormone signal transduction were significantly regulated by uniconazole compared with that by control (distilled water) under drought stress. Numerous genes were differentially expressed to increase chlorophyll content, enhance photosynthesis, regulate carbon–nitrogen metabolism-related enzyme activities, and alter endogenous hormone levels. Thus, uniconazole regulated physiological and molecular characteristics of photosynthesis, carbon–nitrogen metabolism, and plant hormone signal transduction to enhance drought resistance in industrial hemp.
format article
author Ying Jiang
Yufeng Sun
Dianfeng Zheng
Chengwei Han
Kun Cao
Lei Xu
Shuxia Liu
Yanyong Cao
Naijie Feng
author_facet Ying Jiang
Yufeng Sun
Dianfeng Zheng
Chengwei Han
Kun Cao
Lei Xu
Shuxia Liu
Yanyong Cao
Naijie Feng
author_sort Ying Jiang
title Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
title_short Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
title_full Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
title_fullStr Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
title_full_unstemmed Physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (Cannabis sativa L.)
title_sort physiological and transcriptome analyses for assessing the effects of exogenous uniconazole on drought tolerance in hemp (cannabis sativa l.)
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/3a9378c820824a2da4650b6b0ec23629
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