Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease

Abstract Experimental methods that allow examination of the intact vascular network of large organs, such as the human placenta are limited, preventing adequate comparison of normal and abnormal vascular development in pregnancy disease. Our aims were (i) to devise an effective technique for three-d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Toluwalope O. Junaid, Robert S. Bradley, Rohan M. Lewis, John D. Aplin, Edward D. Johnstone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3a96e1dbebb749ecb8a484fba9d4fd9c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3a96e1dbebb749ecb8a484fba9d4fd9c
record_format	dspace
spelling	oai:doaj.org-article:3a96e1dbebb749ecb8a484fba9d4fd9c2021-12-02T15:04:57ZWhole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease10.1038/s41598-017-04379-02045-2322https://doaj.org/article/3a96e1dbebb749ecb8a484fba9d4fd9c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04379-0https://doaj.org/toc/2045-2322Abstract Experimental methods that allow examination of the intact vascular network of large organs, such as the human placenta are limited, preventing adequate comparison of normal and abnormal vascular development in pregnancy disease. Our aims were (i) to devise an effective technique for three-dimensional analyses of human placental vessels; (ii) demonstrate the utility of the technique in the comparison of placental vessel networks in normal and fetal growth restriction (FGR) complicated pregnancies. Radiopaque plastic vessel networks of normal and FGR placentas (n = 12/group) were created by filling the vessels with resin and corroding the surrounding tissues. Subsequently, each model was scanned in a microCT scanner, reconstructed into three-dimensional virtual objects and analysed in visualisation programmes. MicroCT imaging of the models defined vessel anatomy to our analyses threshold of 100 µm diameter. Median vessel length density was significantly shorter in arterial but longer in venous FGR networks compared to normals. No significant differences were demonstrable in arterial or venous tortuosity, diameter or branch density. This study demonstrates the potential effectiveness of microCT for ex-vivo examination of human placental vessel morphology. Our findings show significant discrepancies in vessel length density in FGR placentas. The effects on fetoplacental blood flow, and hence nutrient transfer to the fetus, are unknown.Toluwalope O. JunaidRobert S. BradleyRohan M. LewisJohn D. AplinEdward D. JohnstoneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Toluwalope O. Junaid Robert S. Bradley Rohan M. Lewis John D. Aplin Edward D. Johnstone Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
description	Abstract Experimental methods that allow examination of the intact vascular network of large organs, such as the human placenta are limited, preventing adequate comparison of normal and abnormal vascular development in pregnancy disease. Our aims were (i) to devise an effective technique for three-dimensional analyses of human placental vessels; (ii) demonstrate the utility of the technique in the comparison of placental vessel networks in normal and fetal growth restriction (FGR) complicated pregnancies. Radiopaque plastic vessel networks of normal and FGR placentas (n = 12/group) were created by filling the vessels with resin and corroding the surrounding tissues. Subsequently, each model was scanned in a microCT scanner, reconstructed into three-dimensional virtual objects and analysed in visualisation programmes. MicroCT imaging of the models defined vessel anatomy to our analyses threshold of 100 µm diameter. Median vessel length density was significantly shorter in arterial but longer in venous FGR networks compared to normals. No significant differences were demonstrable in arterial or venous tortuosity, diameter or branch density. This study demonstrates the potential effectiveness of microCT for ex-vivo examination of human placental vessel morphology. Our findings show significant discrepancies in vessel length density in FGR placentas. The effects on fetoplacental blood flow, and hence nutrient transfer to the fetus, are unknown.
format	article
author	Toluwalope O. Junaid Robert S. Bradley Rohan M. Lewis John D. Aplin Edward D. Johnstone
author_facet	Toluwalope O. Junaid Robert S. Bradley Rohan M. Lewis John D. Aplin Edward D. Johnstone
author_sort	Toluwalope O. Junaid
title	Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
title_short	Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
title_full	Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
title_fullStr	Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
title_full_unstemmed	Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
title_sort	whole organ vascular casting and microct examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3a96e1dbebb749ecb8a484fba9d4fd9c
work_keys_str_mv	AT toluwalopeojunaid wholeorganvascularcastingandmicroctexaminationofthehumanplacentalvasculartreerevealsnovelalterationsassociatedwithpregnancydisease AT robertsbradley wholeorganvascularcastingandmicroctexaminationofthehumanplacentalvasculartreerevealsnovelalterationsassociatedwithpregnancydisease AT rohanmlewis wholeorganvascularcastingandmicroctexaminationofthehumanplacentalvasculartreerevealsnovelalterationsassociatedwithpregnancydisease AT johndaplin wholeorganvascularcastingandmicroctexaminationofthehumanplacentalvasculartreerevealsnovelalterationsassociatedwithpregnancydisease AT edwarddjohnstone wholeorganvascularcastingandmicroctexaminationofthehumanplacentalvasculartreerevealsnovelalterationsassociatedwithpregnancydisease
_version_	1718388948153139200

Whole organ vascular casting and microCT examination of the human placental vascular tree reveals novel alterations associated with pregnancy disease

Ejemplares similares