Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients

Introduction: Medullary thyroid carcinoma (MTC) comprises nearly 5% of all cases of thyroid cancer (TC). Aberrant activation of RET (rearranged during transfection) signaling via somatic mutations is the basic molecular mechanism of MTC tumorigenicity. In this study, we determined the incidence of R...

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Autores principales:	Elham Shakiba, Mehdi Hedayati, Ahmad Majd, Monireh Movahedi
Formato:	article
Lenguaje:	EN
Publicado:	Zabol University of Medical sciences 2020
Materias:	ret gene somatic mutations iranian patient medullary thyroid carcinoma thyroid caner Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/3a996ca7460f4e99877fcbeee1b8fc32
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spelling	oai:doaj.org-article:3a996ca7460f4e99877fcbeee1b8fc322021-11-17T09:54:38ZSomatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients2476-664X10.34172/ijbsm.2020.19https://doaj.org/article/3a996ca7460f4e99877fcbeee1b8fc322020-09-01T00:00:00Zhttp://ijbsm.zbmu.ac.ir/PDF/ijbsm-20430https://doaj.org/toc/2476-664XIntroduction: Medullary thyroid carcinoma (MTC) comprises nearly 5% of all cases of thyroid cancer (TC). Aberrant activation of RET (rearranged during transfection) signaling via somatic mutations is the basic molecular mechanism of MTC tumorigenicity. In this study, we determined the incidence of RET gene mutations in exons 10, 11, and 16 in Iranian patients. Methods: A total of 33 patients undergoing thyroidectomy at Imam Khomeini hospital of Tehran, Iran and diagnosed with MTC were enrolled. For investigating mutations in exons 10, 11, and 16, DNA was extracted from tumor tissues, and the genes were amplified by polymerase chain reaction (PCR) and then sequenced. Results: Out of 33 patients, 20 (60.6%) subjects had mutations in one of the examined exons (10, 11, and 16). According to our results, the "ATG918ACG" mutation in codon 918 had the highest rate. Conclusion: Testing RET mutations can be beneficial in clinical evaluation and treatment management of MTC patients.Elham ShakibaMehdi HedayatiAhmad MajdMonireh MovahediZabol University of Medical sciencesarticleret gene somatic mutations iranian patient medullary thyroid carcinoma thyroid caner Medicine (General)R5-920ENInternational Journal of Basic Science in Medicine, Vol 5, Iss 3, Pp 108-113 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	ret gene somatic mutations iranian patient medullary thyroid carcinoma thyroid caner Medicine (General) R5-920
spellingShingle	ret gene somatic mutations iranian patient medullary thyroid carcinoma thyroid caner Medicine (General) R5-920 Elham Shakiba Mehdi Hedayati Ahmad Majd Monireh Movahedi Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
description	Introduction: Medullary thyroid carcinoma (MTC) comprises nearly 5% of all cases of thyroid cancer (TC). Aberrant activation of RET (rearranged during transfection) signaling via somatic mutations is the basic molecular mechanism of MTC tumorigenicity. In this study, we determined the incidence of RET gene mutations in exons 10, 11, and 16 in Iranian patients. Methods: A total of 33 patients undergoing thyroidectomy at Imam Khomeini hospital of Tehran, Iran and diagnosed with MTC were enrolled. For investigating mutations in exons 10, 11, and 16, DNA was extracted from tumor tissues, and the genes were amplified by polymerase chain reaction (PCR) and then sequenced. Results: Out of 33 patients, 20 (60.6%) subjects had mutations in one of the examined exons (10, 11, and 16). According to our results, the "ATG918ACG" mutation in codon 918 had the highest rate. Conclusion: Testing RET mutations can be beneficial in clinical evaluation and treatment management of MTC patients.
format	article
author	Elham Shakiba Mehdi Hedayati Ahmad Majd Monireh Movahedi
author_facet	Elham Shakiba Mehdi Hedayati Ahmad Majd Monireh Movahedi
author_sort	Elham Shakiba
title	Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
title_short	Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
title_full	Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
title_fullStr	Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
title_full_unstemmed	Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients
title_sort	somatic mutations in exons 10, 11, and 16 of the ret protooncogene in medullary thyroid carcinoma patients
publisher	Zabol University of Medical sciences
publishDate	2020
url	https://doaj.org/article/3a996ca7460f4e99877fcbeee1b8fc32
work_keys_str_mv	AT elhamshakiba somaticmutationsinexons1011and16oftheretprotooncogeneinmedullarythyroidcarcinomapatients AT mehdihedayati somaticmutationsinexons1011and16oftheretprotooncogeneinmedullarythyroidcarcinomapatients AT ahmadmajd somaticmutationsinexons1011and16oftheretprotooncogeneinmedullarythyroidcarcinomapatients AT monirehmovahedi somaticmutationsinexons1011and16oftheretprotooncogeneinmedullarythyroidcarcinomapatients
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Somatic Mutations in Exons 10, 11, and 16 of the RET Protooncogene in Medullary Thyroid Carcinoma Patients

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