Choice of method of place cell classification determines the population of cells identified.

Place cells, spatially responsive hippocampal cells, provide the neural substrate supporting navigation and spatial memory. Historically most studies of these neurons have used electrophysiological recordings from implanted electrodes but optical methods, measuring intracellular calcium, are becomin...

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Autores principales:	Dori M Grijseels, Kira Shaw, Caswell Barry, Catherine N Hall
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3aa950f065494a04bb3272874ee235dd
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spelling	oai:doaj.org-article:3aa950f065494a04bb3272874ee235dd2021-12-02T19:57:34ZChoice of method of place cell classification determines the population of cells identified.1553-734X1553-735810.1371/journal.pcbi.1008835https://doaj.org/article/3aa950f065494a04bb3272874ee235dd2021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1008835https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Place cells, spatially responsive hippocampal cells, provide the neural substrate supporting navigation and spatial memory. Historically most studies of these neurons have used electrophysiological recordings from implanted electrodes but optical methods, measuring intracellular calcium, are becoming increasingly common. Several methods have been proposed as a means to identify place cells based on their calcium activity but there is no common standard and it is unclear how reliable different approaches are. Here we tested four methods that have previously been applied to two-photon hippocampal imaging or electrophysiological data, using both model datasets and real imaging data. These methods use different parameters to identify place cells, including the peak activity in the place field, compared to other locations (the Peak method); the stability of cells' activity over repeated traversals of an environment (Stability method); a combination of these parameters with the size of the place field (Combination method); and the spatial information held by the cells (Information method). The methods performed differently from each other on both model and real data. In real datasets, vastly different numbers of place cells were identified using the four methods, with little overlap between the populations identified as place cells. Therefore, choice of place cell detection method dramatically affects the number and properties of identified cells. Ultimately, we recommend the Peak method be used in future studies to identify place cell populations, as this method is robust to moderate variations in place field within a session, and makes no inherent assumptions about the spatial information in place fields, unless there is an explicit theoretical reason for detecting cells with more narrowly defined properties.Dori M GrijseelsKira ShawCaswell BarryCatherine N HallPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 7, p e1008835 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Dori M Grijseels Kira Shaw Caswell Barry Catherine N Hall Choice of method of place cell classification determines the population of cells identified.
description	Place cells, spatially responsive hippocampal cells, provide the neural substrate supporting navigation and spatial memory. Historically most studies of these neurons have used electrophysiological recordings from implanted electrodes but optical methods, measuring intracellular calcium, are becoming increasingly common. Several methods have been proposed as a means to identify place cells based on their calcium activity but there is no common standard and it is unclear how reliable different approaches are. Here we tested four methods that have previously been applied to two-photon hippocampal imaging or electrophysiological data, using both model datasets and real imaging data. These methods use different parameters to identify place cells, including the peak activity in the place field, compared to other locations (the Peak method); the stability of cells' activity over repeated traversals of an environment (Stability method); a combination of these parameters with the size of the place field (Combination method); and the spatial information held by the cells (Information method). The methods performed differently from each other on both model and real data. In real datasets, vastly different numbers of place cells were identified using the four methods, with little overlap between the populations identified as place cells. Therefore, choice of place cell detection method dramatically affects the number and properties of identified cells. Ultimately, we recommend the Peak method be used in future studies to identify place cell populations, as this method is robust to moderate variations in place field within a session, and makes no inherent assumptions about the spatial information in place fields, unless there is an explicit theoretical reason for detecting cells with more narrowly defined properties.
format	article
author	Dori M Grijseels Kira Shaw Caswell Barry Catherine N Hall
author_facet	Dori M Grijseels Kira Shaw Caswell Barry Catherine N Hall
author_sort	Dori M Grijseels
title	Choice of method of place cell classification determines the population of cells identified.
title_short	Choice of method of place cell classification determines the population of cells identified.
title_full	Choice of method of place cell classification determines the population of cells identified.
title_fullStr	Choice of method of place cell classification determines the population of cells identified.
title_full_unstemmed	Choice of method of place cell classification determines the population of cells identified.
title_sort	choice of method of place cell classification determines the population of cells identified.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3aa950f065494a04bb3272874ee235dd
work_keys_str_mv	AT dorimgrijseels choiceofmethodofplacecellclassificationdeterminesthepopulationofcellsidentified AT kirashaw choiceofmethodofplacecellclassificationdeterminesthepopulationofcellsidentified AT caswellbarry choiceofmethodofplacecellclassificationdeterminesthepopulationofcellsidentified AT catherinenhall choiceofmethodofplacecellclassificationdeterminesthepopulationofcellsidentified
_version_	1718375770829619200

Choice of method of place cell classification determines the population of cells identified.

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