Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.

This study aims to demonstrate how the state of chronic hyperglycemia from experimental Diabetes Mellitus can influence the homeostatic imbalance of tendons and, consequently, lead to the characteristics of tendinopathy. Twenty animals were randomly divided into two experimental groups: control grou...

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Autores principales:	Rodrigo R de Oliveira, Conceição S Martins, Yuri R Rocha, Allysson B R Braga, Rômulo M Mattos, Fábio Hecht, Gerly A C Brito, Luiz E Nasciutti
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3aaeb09a6be046f985ecfd317737e6b3
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spelling	oai:doaj.org-article:3aaeb09a6be046f985ecfd317737e6b32021-11-18T08:51:54ZExperimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.1932-620310.1371/journal.pone.0074942https://doaj.org/article/3aaeb09a6be046f985ecfd317737e6b32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24130676/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203This study aims to demonstrate how the state of chronic hyperglycemia from experimental Diabetes Mellitus can influence the homeostatic imbalance of tendons and, consequently, lead to the characteristics of tendinopathy. Twenty animals were randomly divided into two experimental groups: control group, consisting of healthy rats and diabetic group constituted by rats induced to Diabetes Mellitus I. After twenty-four days of the induction of Diabetes type I, the Achilles tendon were removed for morphological evaluation, cellularity, number and cross-sectional area of blood vessel, immunohistochemistry for Collagen type I, VEGF and NF-κB nuclear localization sequence (NLS) and nitrate and nitrite level. The Achilles tendon thickness (µm/100g) of diabetic animals was significantly increased and, similarly, an increase was observed in the density of fibrocytes and mast cells in the tendons of the diabetic group. The average number of blood vessels per field, in peritendinous tissue, was statistically higher in the diabetic group 3.39 (2.98) vessels/field when compared to the control group 0.89 (1.68) vessels/field p = 0.001 and in the intratendinous region, it was observed that blood vessels were extremely rare in the control group 0.035 (0.18) vessels/field and were often present in the tendons of the diabetic group 0.89 (0.99) vessels/field. The immunohistochemistry analysis identified higher density of type 1 collagen and increased expression of VEGF as well as increased immunostaining for NFκB p50 NLS in the nucleus in Achilles tendon of the diabetic group when compared to the control group. Higher levels of nitrite/nitrate were observed in the experimental group induced to diabetes. We conclude that experimental DM induces notable structural, inflammatory and vascular changes in the Achilles tendon which are compatible with the process of chronic tendinopathy.Rodrigo R de OliveiraConceição S MartinsYuri R RochaAllysson B R BragaRômulo M MattosFábio HechtGerly A C BritoLuiz E NasciuttiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e74942 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rodrigo R de Oliveira Conceição S Martins Yuri R Rocha Allysson B R Braga Rômulo M Mattos Fábio Hecht Gerly A C Brito Luiz E Nasciutti Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
description	This study aims to demonstrate how the state of chronic hyperglycemia from experimental Diabetes Mellitus can influence the homeostatic imbalance of tendons and, consequently, lead to the characteristics of tendinopathy. Twenty animals were randomly divided into two experimental groups: control group, consisting of healthy rats and diabetic group constituted by rats induced to Diabetes Mellitus I. After twenty-four days of the induction of Diabetes type I, the Achilles tendon were removed for morphological evaluation, cellularity, number and cross-sectional area of blood vessel, immunohistochemistry for Collagen type I, VEGF and NF-κB nuclear localization sequence (NLS) and nitrate and nitrite level. The Achilles tendon thickness (µm/100g) of diabetic animals was significantly increased and, similarly, an increase was observed in the density of fibrocytes and mast cells in the tendons of the diabetic group. The average number of blood vessels per field, in peritendinous tissue, was statistically higher in the diabetic group 3.39 (2.98) vessels/field when compared to the control group 0.89 (1.68) vessels/field p = 0.001 and in the intratendinous region, it was observed that blood vessels were extremely rare in the control group 0.035 (0.18) vessels/field and were often present in the tendons of the diabetic group 0.89 (0.99) vessels/field. The immunohistochemistry analysis identified higher density of type 1 collagen and increased expression of VEGF as well as increased immunostaining for NFκB p50 NLS in the nucleus in Achilles tendon of the diabetic group when compared to the control group. Higher levels of nitrite/nitrate were observed in the experimental group induced to diabetes. We conclude that experimental DM induces notable structural, inflammatory and vascular changes in the Achilles tendon which are compatible with the process of chronic tendinopathy.
format	article
author	Rodrigo R de Oliveira Conceição S Martins Yuri R Rocha Allysson B R Braga Rômulo M Mattos Fábio Hecht Gerly A C Brito Luiz E Nasciutti
author_facet	Rodrigo R de Oliveira Conceição S Martins Yuri R Rocha Allysson B R Braga Rômulo M Mattos Fábio Hecht Gerly A C Brito Luiz E Nasciutti
author_sort	Rodrigo R de Oliveira
title	Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
title_short	Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
title_full	Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
title_fullStr	Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
title_full_unstemmed	Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.
title_sort	experimental diabetes induces structural, inflammatory and vascular changes of achilles tendons.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3aaeb09a6be046f985ecfd317737e6b3
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Experimental diabetes induces structural, inflammatory and vascular changes of Achilles tendons.

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