Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs

Porcine circovirus associated diseases (PCVAD) caused by porcine circovirus type-2 (PCV-2) are emerging viral diseases with unfavourable effects on animal health and swine economy. We have a lot of information regarding the changes in the lymphoid organs and spleen in PCV-2 infected pigs whereas the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S. Vijayaragavan, B. Dhanush Krishna, I. S. Sajitha, P. M. Priya, R. Anoopraj, S.S. Devi, Chintu Ravishankar, C. Divya, Safeer M. Saifudeen
Formato:	article
Lenguaje:	EN
Publicado:	Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2021
Materias:	pcv-2 polymerase chain reaction bone marrow m: e ratio Animal biochemistry QP501-801 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/3ab20ed894dd46c49979e679d99f017d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3ab20ed894dd46c49979e679d99f017d
record_format	dspace
spelling	oai:doaj.org-article:3ab20ed894dd46c49979e679d99f017d2021-11-15T13:40:50ZMyeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs10.51966/jvas.2021.52.3.250-2560971-07012582-0605https://doaj.org/article/3ab20ed894dd46c49979e679d99f017d2021-07-01T00:00:00Zhttps://www.jvas.in/public_html/upload/article_file/article_file_r0qisz.pdf?t=r0qiszhttps://doaj.org/toc/0971-0701https://doaj.org/toc/2582-0605Porcine circovirus associated diseases (PCVAD) caused by porcine circovirus type-2 (PCV-2) are emerging viral diseases with unfavourable effects on animal health and swine economy. We have a lot of information regarding the changes in the lymphoid organs and spleen in PCV-2 infected pigs whereas the reason for anaemic changes in the carcasses and the pathological effects of PCV-2 in bone marrow are still not well studied. Hence, an extensive study to identify the changes in myeloid and erythroid cells of bone marrow in PCV-2 infected pigs was carried out. Myeloid and erythroid series of cells were counted and analysed from the freshly collected bone marrow cytological smears from the PCV-2 suspected samples. Later, PCV-2 infection was confirmed by polymerase chain reaction (PCR) and characteristic histopathological findings. The PCR yielded an amplicon of ~ 481 bp product and those positive cases were selected for determining the Myeloid to Erythroid ratio (M : E ratio). However, values did not significantly differ in any of the cellular components between PCV-2 positive animals and PCV-2 negative animals which indicated that the bone marrow was not the specific target organ for PCV-2 viral infections. However, increased lympho-histiocytic and plasmacytic infiltration was noticed in both lymphoid and non-lymphoid organs. These characteristic features of PCV-2 infection could be considered as a major reason for increased proliferation of myeloid cells. S. VijayaragavanB. Dhanush KrishnaI. S. SajithaP. M. PriyaR. AnooprajS.S. DeviChintu RavishankarC. DivyaSafeer M. SaifudeenDirector of Academics and Research, Kerala Veterinary and Animal Sciences Universityarticlepcv-2polymerase chain reactionbone marrow m: e ratioAnimal biochemistryQP501-801Science (General)Q1-390ENJournal of Veterinary and Animal Sciences, Vol 52, Iss 3, Pp 250-256 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pcv-2 polymerase chain reaction bone marrow m: e ratio Animal biochemistry QP501-801 Science (General) Q1-390
spellingShingle	pcv-2 polymerase chain reaction bone marrow m: e ratio Animal biochemistry QP501-801 Science (General) Q1-390 S. Vijayaragavan B. Dhanush Krishna I. S. Sajitha P. M. Priya R. Anoopraj S.S. Devi Chintu Ravishankar C. Divya Safeer M. Saifudeen Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
description	Porcine circovirus associated diseases (PCVAD) caused by porcine circovirus type-2 (PCV-2) are emerging viral diseases with unfavourable effects on animal health and swine economy. We have a lot of information regarding the changes in the lymphoid organs and spleen in PCV-2 infected pigs whereas the reason for anaemic changes in the carcasses and the pathological effects of PCV-2 in bone marrow are still not well studied. Hence, an extensive study to identify the changes in myeloid and erythroid cells of bone marrow in PCV-2 infected pigs was carried out. Myeloid and erythroid series of cells were counted and analysed from the freshly collected bone marrow cytological smears from the PCV-2 suspected samples. Later, PCV-2 infection was confirmed by polymerase chain reaction (PCR) and characteristic histopathological findings. The PCR yielded an amplicon of ~ 481 bp product and those positive cases were selected for determining the Myeloid to Erythroid ratio (M : E ratio). However, values did not significantly differ in any of the cellular components between PCV-2 positive animals and PCV-2 negative animals which indicated that the bone marrow was not the specific target organ for PCV-2 viral infections. However, increased lympho-histiocytic and plasmacytic infiltration was noticed in both lymphoid and non-lymphoid organs. These characteristic features of PCV-2 infection could be considered as a major reason for increased proliferation of myeloid cells.
format	article
author	S. Vijayaragavan B. Dhanush Krishna I. S. Sajitha P. M. Priya R. Anoopraj S.S. Devi Chintu Ravishankar C. Divya Safeer M. Saifudeen
author_facet	S. Vijayaragavan B. Dhanush Krishna I. S. Sajitha P. M. Priya R. Anoopraj S.S. Devi Chintu Ravishankar C. Divya Safeer M. Saifudeen
author_sort	S. Vijayaragavan
title	Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
title_short	Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
title_full	Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
title_fullStr	Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
title_full_unstemmed	Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs
title_sort	myeloid to erythroid (m: e) ratio in the evaluation of bone marrow cytology of porcine circovirus type 2 affected pigs
publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
publishDate	2021
url	https://doaj.org/article/3ab20ed894dd46c49979e679d99f017d
work_keys_str_mv	AT svijayaragavan myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT bdhanushkrishna myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT issajitha myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT pmpriya myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT ranoopraj myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT ssdevi myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT chinturavishankar myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT cdivya myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs AT safeermsaifudeen myeloidtoerythroidmeratiointheevaluationofbonemarrowcytologyofporcinecircovirustype2affectedpigs
_version_	1718428427370889216

Myeloid to Erythroid (M: E) ratio in the evaluation of bone marrow cytology of Porcine Circovirus type 2 affected pigs

Ejemplares similares