Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.

Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.

The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In Hevea brasiliensis, ERF genes have been identified by RNA sequencing. This study set out to validate the number of HbERF genes, and identify ERF genes involv...

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Autores principales: Piyanuch Piyatrakul, Meng Yang, Riza-Arief Putranto, Julien Pirrello, Florence Dessailly, Songnian Hu, Marilyne Summo, Kannikar Theeravatanasuk, Julie Leclercq, Kuswanhadi, Pascal Montoro
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/3ab8d3c684be472fa50f033ffde54802
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spelling oai:doaj.org-article:3ab8d3c684be472fa50f033ffde548022021-11-11T08:21:08ZSequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.1932-620310.1371/journal.pone.0099367https://doaj.org/article/3ab8d3c684be472fa50f033ffde548022014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24971876/?tool=EBIhttps://doaj.org/toc/1932-6203The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In Hevea brasiliensis, ERF genes have been identified by RNA sequencing. This study set out to validate the number of HbERF genes, and identify ERF genes involved in the regulation of latex cell metabolism. A comprehensive Hevea transcriptome was improved using additional RNA reads from reproductive tissues. Newly assembled contigs were annotated in the Gene Ontology database and were assigned to 3 main categories. The AP2/ERF superfamily is the third most represented compared with other transcription factor families. A comparison with genomic scaffolds led to an estimation of 114 AP2/ERF genes and 1 soloist in Hevea brasiliensis. Based on a phylogenetic analysis, functions were predicted for 26 HbERF genes. A relative transcript abundance analysis was performed by real-time RT-PCR in various tissues. Transcripts of ERFs from group I and VIII were very abundant in all tissues while those of group VII were highly accumulated in latex cells. Seven of the thirty-five ERF expression marker genes were highly expressed in latex. Subcellular localization and transactivation analyses suggested that HbERF-VII candidate genes encoded functional transcription factors.Piyanuch PiyatrakulMeng YangRiza-Arief PutrantoJulien PirrelloFlorence DessaillySongnian HuMarilyne SummoKannikar TheeravatanasukJulie LeclercqKuswanhadiPascal MontoroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e99367 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Piyanuch Piyatrakul
Meng Yang
Riza-Arief Putranto
Julien Pirrello
Florence Dessailly
Songnian Hu
Marilyne Summo
Kannikar Theeravatanasuk
Julie Leclercq
Kuswanhadi
Pascal Montoro
Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
description The AP2/ERF superfamily encodes transcription factors that play a key role in plant development and responses to abiotic and biotic stress. In Hevea brasiliensis, ERF genes have been identified by RNA sequencing. This study set out to validate the number of HbERF genes, and identify ERF genes involved in the regulation of latex cell metabolism. A comprehensive Hevea transcriptome was improved using additional RNA reads from reproductive tissues. Newly assembled contigs were annotated in the Gene Ontology database and were assigned to 3 main categories. The AP2/ERF superfamily is the third most represented compared with other transcription factor families. A comparison with genomic scaffolds led to an estimation of 114 AP2/ERF genes and 1 soloist in Hevea brasiliensis. Based on a phylogenetic analysis, functions were predicted for 26 HbERF genes. A relative transcript abundance analysis was performed by real-time RT-PCR in various tissues. Transcripts of ERFs from group I and VIII were very abundant in all tissues while those of group VII were highly accumulated in latex cells. Seven of the thirty-five ERF expression marker genes were highly expressed in latex. Subcellular localization and transactivation analyses suggested that HbERF-VII candidate genes encoded functional transcription factors.
format article
author Piyanuch Piyatrakul
Meng Yang
Riza-Arief Putranto
Julien Pirrello
Florence Dessailly
Songnian Hu
Marilyne Summo
Kannikar Theeravatanasuk
Julie Leclercq
Kuswanhadi
Pascal Montoro
author_facet Piyanuch Piyatrakul
Meng Yang
Riza-Arief Putranto
Julien Pirrello
Florence Dessailly
Songnian Hu
Marilyne Summo
Kannikar Theeravatanasuk
Julie Leclercq
Kuswanhadi
Pascal Montoro
author_sort Piyanuch Piyatrakul
title Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
title_short Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
title_full Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
title_fullStr Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
title_full_unstemmed Sequence and expression analyses of ethylene response factors highly expressed in latex cells from Hevea brasiliensis.
title_sort sequence and expression analyses of ethylene response factors highly expressed in latex cells from hevea brasiliensis.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/3ab8d3c684be472fa50f033ffde54802
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