Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.

Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Subash C B Gopinath, Koichi Awazu, Makoto Fujimaki, Kazufumi Shimizu, Takayuki Shima
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3aba2acf58004eed8dd812f542e0e838
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3aba2acf58004eed8dd812f542e0e838
record_format	dspace
spelling	oai:doaj.org-article:3aba2acf58004eed8dd812f542e0e8382021-11-18T07:37:46ZObservations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.1932-620310.1371/journal.pone.0069121https://doaj.org/article/3aba2acf58004eed8dd812f542e0e8382013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23874887/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could detect 8×10(5) PFU/ml (40 pg/µl) intact A/Udorn/307/1972 and 120 pg/µl A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as the virtual detection level was increased to 8×10(4) PFU/ml A/Udorn/307/1972. Immuno-AuNPs were then complexed with viruses to permit direct observation, and they formed a ring of confined nanodots on the membrane of both intact and detergent-treated viruses as directly visualized by scanning electron microscopy. With this complex the detection limit was improved further to 8×10(3) PFU/ml on anti-rabbit IgG immobilized sensing plate. These strategies introduce methods for observing trapped intact viruses on the sensing plates generated for optical systems.Subash C B GopinathKoichi AwazuMakoto FujimakiKazufumi ShimizuTakayuki ShimaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69121 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Subash C B Gopinath Koichi Awazu Makoto Fujimaki Kazufumi Shimizu Takayuki Shima Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
description	Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus to detect viruses on a sensing plate designed for an evanescent field-coupled waveguide-mode sensor. Experiments were conducted using human influenza A/H3N2 strains, and immuno-AuNP could detect 8×10(5) PFU/ml (40 pg/µl) intact A/Udorn/307/1972 and 120 pg/µl A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as the virtual detection level was increased to 8×10(4) PFU/ml A/Udorn/307/1972. Immuno-AuNPs were then complexed with viruses to permit direct observation, and they formed a ring of confined nanodots on the membrane of both intact and detergent-treated viruses as directly visualized by scanning electron microscopy. With this complex the detection limit was improved further to 8×10(3) PFU/ml on anti-rabbit IgG immobilized sensing plate. These strategies introduce methods for observing trapped intact viruses on the sensing plates generated for optical systems.
format	article
author	Subash C B Gopinath Koichi Awazu Makoto Fujimaki Kazufumi Shimizu Takayuki Shima
author_facet	Subash C B Gopinath Koichi Awazu Makoto Fujimaki Kazufumi Shimizu Takayuki Shima
author_sort	Subash C B Gopinath
title	Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
title_short	Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
title_full	Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
title_fullStr	Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
title_full_unstemmed	Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
title_sort	observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3aba2acf58004eed8dd812f542e0e838
work_keys_str_mv	AT subashcbgopinath observationsofimmunogoldconjugatesoninfluenzavirusesusingwaveguidemodesensors AT koichiawazu observationsofimmunogoldconjugatesoninfluenzavirusesusingwaveguidemodesensors AT makotofujimaki observationsofimmunogoldconjugatesoninfluenzavirusesusingwaveguidemodesensors AT kazufumishimizu observationsofimmunogoldconjugatesoninfluenzavirusesusingwaveguidemodesensors AT takayukishima observationsofimmunogoldconjugatesoninfluenzavirusesusingwaveguidemodesensors
_version_	1718423153262198784

Observations of immuno-gold conjugates on influenza viruses using waveguide-mode sensors.

Ejemplares similares