Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells
Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular we...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:3aba2dec24014ae2b6b19a250ba9e1c62021-11-18T09:27:52ZStructural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells2296-418510.3389/fbioe.2021.794818https://doaj.org/article/3aba2dec24014ae2b6b19a250ba9e1c62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.794818/fullhttps://doaj.org/toc/2296-4185Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe2+-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries.Yu PeiYu PeiShengtao YangShengtao YangZhenbang XiaoZhenbang XiaoChunxia ZhouChunxia ZhouPengzhi HongPengzhi HongZhong-Ji QianZhong-Ji QianFrontiers Media S.A.articleGelidium crinalesulfated polysaccharidestructural characterizationanti-oxidationanti-inflammationBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Gelidium crinale sulfated polysaccharide structural characterization anti-oxidation anti-inflammation Biotechnology TP248.13-248.65 |
| spellingShingle |
Gelidium crinale sulfated polysaccharide structural characterization anti-oxidation anti-inflammation Biotechnology TP248.13-248.65 Yu Pei Yu Pei Shengtao Yang Shengtao Yang Zhenbang Xiao Zhenbang Xiao Chunxia Zhou Chunxia Zhou Pengzhi Hong Pengzhi Hong Zhong-Ji Qian Zhong-Ji Qian Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| description |
Gelidium crinale, the red algae belonging to Geliaceae Gelidium, is a traditional edible and industrial alga in China. A sulfated polysaccharide (GNP) is successfully separated from Gelidium crinale by acid extraction and two-step column chromatography. Chemical analysis showed that the molecular weight of GNP was 25.8 kDa and the monosaccharide composition had the highest galactose content and confirmed the presence and content (16.5%) of sulfate by Fourier transform infrared spectroscopy (FT-IR) spectrometry as well as barium chloride-gelatin methods. In addition, the effect of GNP on lipopolysaccharide (LPS)-induced oxidative stress and inflammation in macrophages was also evaluated. The research results showed that GNP had fairly strong scavenging activities on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical, hydroxyl radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and had Fe2+-chelating ability in a dose-dependent manner. At the same time, it significantly inhibits the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the production of pro-inflammatory cytokines in RAW 264.7 cells induced by LPS through blocking the mitogen-activated protein kinase (MAPK)/nuclear factor kappa beta (NF-κB) signaling pathway. These results indicate that GNP may be a latent component anti-inflammation in pharmaceutical and functional food industries. |
| format |
article |
| author |
Yu Pei Yu Pei Shengtao Yang Shengtao Yang Zhenbang Xiao Zhenbang Xiao Chunxia Zhou Chunxia Zhou Pengzhi Hong Pengzhi Hong Zhong-Ji Qian Zhong-Ji Qian |
| author_facet |
Yu Pei Yu Pei Shengtao Yang Shengtao Yang Zhenbang Xiao Zhenbang Xiao Chunxia Zhou Chunxia Zhou Pengzhi Hong Pengzhi Hong Zhong-Ji Qian Zhong-Ji Qian |
| author_sort |
Yu Pei |
| title |
Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| title_short |
Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| title_full |
Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| title_fullStr |
Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| title_full_unstemmed |
Structural Characterization of Sulfated Polysaccharide Isolated From Red Algae (Gelidium crinale) and Antioxidant and Anti-Inflammatory Effects in Macrophage Cells |
| title_sort |
structural characterization of sulfated polysaccharide isolated from red algae (gelidium crinale) and antioxidant and anti-inflammatory effects in macrophage cells |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/3aba2dec24014ae2b6b19a250ba9e1c6 |
| work_keys_str_mv |
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| _version_ |
1718420897708113920 |