Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm

Abstract The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study,...

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Autores principales:	Ge-Hong Sun-Wada, Hiroyuki Tabata, Yoh Wada
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ac42a9a0543489e861ea346d5655c7a
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spelling	oai:doaj.org-article:3ac42a9a0543489e861ea346d5655c7a2021-12-02T19:16:46ZVacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm10.1038/s41598-021-98952-32045-2322https://doaj.org/article/3ac42a9a0543489e861ea346d5655c7a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98952-3https://doaj.org/toc/2045-2322Abstract The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.Ge-Hong Sun-WadaHiroyuki TabataYoh WadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ge-Hong Sun-Wada Hiroyuki Tabata Yoh Wada Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
description	Abstract The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis.
format	article
author	Ge-Hong Sun-Wada Hiroyuki Tabata Yoh Wada
author_facet	Ge-Hong Sun-Wada Hiroyuki Tabata Yoh Wada
author_sort	Ge-Hong Sun-Wada
title	Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
title_short	Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
title_full	Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
title_fullStr	Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
title_full_unstemmed	Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
title_sort	vacuolar-type proton atpase is required for maintenance of apicobasal polarity of embryonic visceral endoderm
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3ac42a9a0543489e861ea346d5655c7a
work_keys_str_mv	AT gehongsunwada vacuolartypeprotonatpaseisrequiredformaintenanceofapicobasalpolarityofembryonicvisceralendoderm AT hiroyukitabata vacuolartypeprotonatpaseisrequiredformaintenanceofapicobasalpolarityofembryonicvisceralendoderm AT yohwada vacuolartypeprotonatpaseisrequiredformaintenanceofapicobasalpolarityofembryonicvisceralendoderm
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Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm

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