Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis

Abstract Streptococcus pneumoniae is responsible for diseases causing major global public health problems, including meningitis, pneumonia and septicaemia. Despite recent advances in antimicrobial therapy, pneumococcal meningitis remains a life-threatening disease. Furthermore, long-term sequelae ar...

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Autores principales:	Guadalupe Gómez-Baena, Richard J. Bennett, Carmen Martínez-Rodríguez, Małgorzata Wnęk, Gavin Laing, Graeme Hickey, Lynn McLean, Robert J. Beynon, Enitan D. Carrol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ac6a44048a749549724f1fe70b48286
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spelling	oai:doaj.org-article:3ac6a44048a749549724f1fe70b482862021-12-02T11:40:41ZQuantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis10.1038/s41598-017-07127-62045-2322https://doaj.org/article/3ac6a44048a749549724f1fe70b482862017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07127-6https://doaj.org/toc/2045-2322Abstract Streptococcus pneumoniae is responsible for diseases causing major global public health problems, including meningitis, pneumonia and septicaemia. Despite recent advances in antimicrobial therapy, pneumococcal meningitis remains a life-threatening disease. Furthermore, long-term sequelae are a major concern for survivors. Hence, a better understanding of the processes occurring in the central nervous system is crucial to the development of more effective management strategies. We used mass spectrometry based quantitative proteomics to identify protein changes in cerebrospinal fluid from children with Streptococcus pneumoniae infection, compared with children admitted to hospital with bacterial meningitis symptoms but negative diagnosis. Samples were analysed, by label free proteomics, in two independent cohorts (cohort 1: cases (n = 8) and hospital controls (n = 4); cohort 2: cases (n = 8), hospital controls (n = 8)). Over 200 human proteins were differentially expressed in each cohort, of which 65% were common to both. Proteins involved in the immune response and exosome signalling were significantly enriched in the infected samples. For a subset of proteins derived from the proteome analysis, we corroborated the proteomics data in a third cohort (hospital controls (n = 15), healthy controls (n = 5), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics findings. Proteomics data are available via ProteomeXchange with identifier PXD004219.Guadalupe Gómez-BaenaRichard J. BennettCarmen Martínez-RodríguezMałgorzata WnękGavin LaingGraeme HickeyLynn McLeanRobert J. BeynonEnitan D. CarrolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guadalupe Gómez-Baena Richard J. Bennett Carmen Martínez-Rodríguez Małgorzata Wnęk Gavin Laing Graeme Hickey Lynn McLean Robert J. Beynon Enitan D. Carrol Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
description	Abstract Streptococcus pneumoniae is responsible for diseases causing major global public health problems, including meningitis, pneumonia and septicaemia. Despite recent advances in antimicrobial therapy, pneumococcal meningitis remains a life-threatening disease. Furthermore, long-term sequelae are a major concern for survivors. Hence, a better understanding of the processes occurring in the central nervous system is crucial to the development of more effective management strategies. We used mass spectrometry based quantitative proteomics to identify protein changes in cerebrospinal fluid from children with Streptococcus pneumoniae infection, compared with children admitted to hospital with bacterial meningitis symptoms but negative diagnosis. Samples were analysed, by label free proteomics, in two independent cohorts (cohort 1: cases (n = 8) and hospital controls (n = 4); cohort 2: cases (n = 8), hospital controls (n = 8)). Over 200 human proteins were differentially expressed in each cohort, of which 65% were common to both. Proteins involved in the immune response and exosome signalling were significantly enriched in the infected samples. For a subset of proteins derived from the proteome analysis, we corroborated the proteomics data in a third cohort (hospital controls (n = 15), healthy controls (n = 5), cases (n = 20)) by automated quantitative western blotting, with excellent agreement with our proteomics findings. Proteomics data are available via ProteomeXchange with identifier PXD004219.
format	article
author	Guadalupe Gómez-Baena Richard J. Bennett Carmen Martínez-Rodríguez Małgorzata Wnęk Gavin Laing Graeme Hickey Lynn McLean Robert J. Beynon Enitan D. Carrol
author_facet	Guadalupe Gómez-Baena Richard J. Bennett Carmen Martínez-Rodríguez Małgorzata Wnęk Gavin Laing Graeme Hickey Lynn McLean Robert J. Beynon Enitan D. Carrol
author_sort	Guadalupe Gómez-Baena
title	Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
title_short	Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
title_full	Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
title_fullStr	Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
title_full_unstemmed	Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
title_sort	quantitative proteomics of cerebrospinal fluid in paediatric pneumococcal meningitis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3ac6a44048a749549724f1fe70b48286
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_version_	1718395574758146048

Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis

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