Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay

Herein we describe the development of a mix-read bioassay based on a three-dimensional (3D) poly ethylene glycol—(PEG)-hydrogel microparticles for the detection of oligonucleotides in complex media. The key steps of hydrogels synthesis and molecular recognition in a 3D polymer network are elucidated...

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Autores principales:	Alessia Mazzarotta, Tania Mariastella Caputo, Edmondo Battista, Paolo Antonio Netti, Filippo Causa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hydrogel microparticles 3D recognition miRNA detection mix-read bioassay Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/3acbca9fc60741d191708097b02c09bb
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spelling	oai:doaj.org-article:3acbca9fc60741d191708097b02c09bb2021-11-25T18:58:24ZHydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay10.3390/s212276711424-8220https://doaj.org/article/3acbca9fc60741d191708097b02c09bb2021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8220/21/22/7671https://doaj.org/toc/1424-8220Herein we describe the development of a mix-read bioassay based on a three-dimensional (3D) poly ethylene glycol—(PEG)-hydrogel microparticles for the detection of oligonucleotides in complex media. The key steps of hydrogels synthesis and molecular recognition in a 3D polymer network are elucidated. The design of the DNA probes and their density in polymer network were opportunely optimized. Furthermore, the diffusion into the polymer was tuned adjusting the polymer concentration and consequently the characteristic mesh size. Upon parameters optimization, 3D-PEG-hydrogels were synthetized in a microfluidic system and provided with fluorescent probe. Target detection occurred by double strand displacement assay associated to fluorescence depletion within the hydrogel microparticle. Proposed 3D-PEG-hydrogel microparticles were designed for miR-143-3p detection. Results showed 3D-hydrogel microparticles with working range comprise between 10<sup>−6</sup>–10<sup>−12</sup> M, had limit of detection of 30 pM and good specificity. Moreover, due to the anti-fouling properties of PEG-hydrogel, the target detection occurred in human serum with performance comparable to that in buffer. Due to the approach versatility, such design could be easily adapted to other short oligonucleotides detection.Alessia MazzarottaTania Mariastella CaputoEdmondo BattistaPaolo Antonio NettiFilippo CausaMDPI AGarticlehydrogel microparticles3D recognitionmiRNA detectionmix-read bioassayChemical technologyTP1-1185ENSensors, Vol 21, Iss 7671, p 7671 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hydrogel microparticles 3D recognition miRNA detection mix-read bioassay Chemical technology TP1-1185
spellingShingle	hydrogel microparticles 3D recognition miRNA detection mix-read bioassay Chemical technology TP1-1185 Alessia Mazzarotta Tania Mariastella Caputo Edmondo Battista Paolo Antonio Netti Filippo Causa Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
description	Herein we describe the development of a mix-read bioassay based on a three-dimensional (3D) poly ethylene glycol—(PEG)-hydrogel microparticles for the detection of oligonucleotides in complex media. The key steps of hydrogels synthesis and molecular recognition in a 3D polymer network are elucidated. The design of the DNA probes and their density in polymer network were opportunely optimized. Furthermore, the diffusion into the polymer was tuned adjusting the polymer concentration and consequently the characteristic mesh size. Upon parameters optimization, 3D-PEG-hydrogels were synthetized in a microfluidic system and provided with fluorescent probe. Target detection occurred by double strand displacement assay associated to fluorescence depletion within the hydrogel microparticle. Proposed 3D-PEG-hydrogel microparticles were designed for miR-143-3p detection. Results showed 3D-hydrogel microparticles with working range comprise between 10<sup>−6</sup>–10<sup>−12</sup> M, had limit of detection of 30 pM and good specificity. Moreover, due to the anti-fouling properties of PEG-hydrogel, the target detection occurred in human serum with performance comparable to that in buffer. Due to the approach versatility, such design could be easily adapted to other short oligonucleotides detection.
format	article
author	Alessia Mazzarotta Tania Mariastella Caputo Edmondo Battista Paolo Antonio Netti Filippo Causa
author_facet	Alessia Mazzarotta Tania Mariastella Caputo Edmondo Battista Paolo Antonio Netti Filippo Causa
author_sort	Alessia Mazzarotta
title	Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
title_short	Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
title_full	Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
title_fullStr	Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
title_full_unstemmed	Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay
title_sort	hydrogel microparticles for fluorescence detection of mirna in mix-read bioassay
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3acbca9fc60741d191708097b02c09bb
work_keys_str_mv	AT alessiamazzarotta hydrogelmicroparticlesforfluorescencedetectionofmirnainmixreadbioassay AT taniamariastellacaputo hydrogelmicroparticlesforfluorescencedetectionofmirnainmixreadbioassay AT edmondobattista hydrogelmicroparticlesforfluorescencedetectionofmirnainmixreadbioassay AT paoloantonionetti hydrogelmicroparticlesforfluorescencedetectionofmirnainmixreadbioassay AT filippocausa hydrogelmicroparticlesforfluorescencedetectionofmirnainmixreadbioassay
_version_	1718410456948801536

Hydrogel Microparticles for Fluorescence Detection of miRNA in Mix-Read Bioassay

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