Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants

Abstract The neurophysiological mechanisms underlying NGF-induced masseter muscle sensitization and sex-related differences in its effect are not well understood in humans. Therefore, this longitudinal cohort study aimed to investigate the effect of NGF injection on the density and expression of sub...

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Autores principales:	Abdelrahman M. Alhilou, Akiko Shimada, Camilla I. Svensson, Peter Svensson, Malin Ernberg, Brian E. Cairns, Nikolaos Christidis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ad27e6f08df4f04bb27d76dd357d1ca
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spelling	oai:doaj.org-article:3ad27e6f08df4f04bb27d76dd357d1ca2021-12-02T18:34:06ZSex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants10.1038/s41598-021-93171-22045-2322https://doaj.org/article/3ad27e6f08df4f04bb27d76dd357d1ca2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93171-2https://doaj.org/toc/2045-2322Abstract The neurophysiological mechanisms underlying NGF-induced masseter muscle sensitization and sex-related differences in its effect are not well understood in humans. Therefore, this longitudinal cohort study aimed to investigate the effect of NGF injection on the density and expression of substance P, NMDA-receptors and NGF by the nerve fibers in the human masseter muscle, to correlate expression with pain characteristics, and to determine any possible sex-related differences in these effects of NGF. The magnitude of NGF-induced mechanical sensitization and pain during oral function was significantly greater in women than in men (P < 0.050). Significant positive correlations were found between nerve fiber expression of NMDA-receptors and peak pain intensity (rs = 0.620, P = 0.048), and expression of NMDA-receptors by putative nociceptors and change in temporal summation pain after glutamate injection (rs = 0.561, P = 0.003). In women, there was a significant inverse relationship between the degree of NGF-induced mechanical sensitization and the change in nerve fiber expression of NMDA-receptors alone (rs = − 0.659, P = 0.013), and in combination with NGF (rs = − 0.764, P = 0.001). In conclusion, women displayed a greater magnitude of NGF-induced mechanical sensitization that also was associated with nerve fibers expression of NMDA-receptors, when compared to men. The present findings suggest that, in women, increased peripheral NMDA-receptor expression could be associated with masseter muscle pain sensitivity.Abdelrahman M. AlhilouAkiko ShimadaCamilla I. SvenssonPeter SvenssonMalin ErnbergBrian E. CairnsNikolaos ChristidisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
description	Abstract The neurophysiological mechanisms underlying NGF-induced masseter muscle sensitization and sex-related differences in its effect are not well understood in humans. Therefore, this longitudinal cohort study aimed to investigate the effect of NGF injection on the density and expression of substance P, NMDA-receptors and NGF by the nerve fibers in the human masseter muscle, to correlate expression with pain characteristics, and to determine any possible sex-related differences in these effects of NGF. The magnitude of NGF-induced mechanical sensitization and pain during oral function was significantly greater in women than in men (P < 0.050). Significant positive correlations were found between nerve fiber expression of NMDA-receptors and peak pain intensity (rs = 0.620, P = 0.048), and expression of NMDA-receptors by putative nociceptors and change in temporal summation pain after glutamate injection (rs = 0.561, P = 0.003). In women, there was a significant inverse relationship between the degree of NGF-induced mechanical sensitization and the change in nerve fiber expression of NMDA-receptors alone (rs = − 0.659, P = 0.013), and in combination with NGF (rs = − 0.764, P = 0.001). In conclusion, women displayed a greater magnitude of NGF-induced mechanical sensitization that also was associated with nerve fibers expression of NMDA-receptors, when compared to men. The present findings suggest that, in women, increased peripheral NMDA-receptor expression could be associated with masseter muscle pain sensitivity.
format	article
author	Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis
author_facet	Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis
author_sort	Abdelrahman M. Alhilou
title	Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
title_short	Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
title_full	Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
title_fullStr	Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
title_full_unstemmed	Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
title_sort	sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3ad27e6f08df4f04bb27d76dd357d1ca
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Sex-related differences in response to masseteric injections of glutamate and nerve growth factor in healthy human participants

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