CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in Vibrio alginolyticus

Vibrio alginolyticus, like other vibrio species, is a widely distributed marine bacterium that is able to outcompete other species in variable niches where diverse organic matters are supplied. However, it remains unclear how these cells sense and adjust metabol...

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Autores principales:	Bing Liu, Qian Gao, Xin Zhang, Huizhen Chen, Ying Zhang, Yuehong Sun, Shan Yang, Chang Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	CsrA <i>Vibrio alginolyticus</i> motility carbohydrate and amino acid metabolism mRNA stability Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3ad913496ad64df1b293b24369d5326c
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spelling	oai:doaj.org-article:3ad913496ad64df1b293b24369d5326c2021-11-25T18:25:34ZCsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>10.3390/microorganisms91123832076-2607https://doaj.org/article/3ad913496ad64df1b293b24369d5326c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2383https://doaj.org/toc/2076-2607<i>Vibrio alginolyticus</i>, like other <i>vibrio</i> species, is a widely distributed marine bacterium that is able to outcompete other species in variable niches where diverse organic matters are supplied. However, it remains unclear how these cells sense and adjust metabolic flux in response to the changing environment. CsrA is a conserved RNA-binding protein that modulates critical cellular processes such as growth ability, central metabolism, virulence, and the stress response in <i>gamma</i>-proteobacteria. Here, we first characterize the <i>csrA</i> homolog in <i>V. alginolyticus</i>. The results show that CsrA activates swarming but not swimming motility, possibly by enhancing the expression of lateral flagellar associated genes. It is also revealed that CsrA modulates the carbon and nitrogen metabolism of <i>V. alginolyticus</i>, as evidenced by a change in the growth kinetics of various carbon and nitrogen sources when CsrA is altered. Quantitative RT-PCR shows that the transcripts of the genes encoding key enzymes involved in the TCA cycle and amino acid metabolism change significantly, which is probably due to the variation in mRNA stability given by CsrA binding. This may suggest that CsrA plays an important role in sensing and responding to environmental changes.Bing LiuQian GaoXin ZhangHuizhen ChenYing ZhangYuehong SunShan YangChang ChenMDPI AGarticleCsrA<i>Vibrio alginolyticus</i>motilitycarbohydrate and amino acid metabolismmRNA stabilityBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2383, p 2383 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CsrA <i>Vibrio alginolyticus</i> motility carbohydrate and amino acid metabolism mRNA stability Biology (General) QH301-705.5
spellingShingle	CsrA <i>Vibrio alginolyticus</i> motility carbohydrate and amino acid metabolism mRNA stability Biology (General) QH301-705.5 Bing Liu Qian Gao Xin Zhang Huizhen Chen Ying Zhang Yuehong Sun Shan Yang Chang Chen CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
description	<i>Vibrio alginolyticus</i>, like other <i>vibrio</i> species, is a widely distributed marine bacterium that is able to outcompete other species in variable niches where diverse organic matters are supplied. However, it remains unclear how these cells sense and adjust metabolic flux in response to the changing environment. CsrA is a conserved RNA-binding protein that modulates critical cellular processes such as growth ability, central metabolism, virulence, and the stress response in <i>gamma</i>-proteobacteria. Here, we first characterize the <i>csrA</i> homolog in <i>V. alginolyticus</i>. The results show that CsrA activates swarming but not swimming motility, possibly by enhancing the expression of lateral flagellar associated genes. It is also revealed that CsrA modulates the carbon and nitrogen metabolism of <i>V. alginolyticus</i>, as evidenced by a change in the growth kinetics of various carbon and nitrogen sources when CsrA is altered. Quantitative RT-PCR shows that the transcripts of the genes encoding key enzymes involved in the TCA cycle and amino acid metabolism change significantly, which is probably due to the variation in mRNA stability given by CsrA binding. This may suggest that CsrA plays an important role in sensing and responding to environmental changes.
format	article
author	Bing Liu Qian Gao Xin Zhang Huizhen Chen Ying Zhang Yuehong Sun Shan Yang Chang Chen
author_facet	Bing Liu Qian Gao Xin Zhang Huizhen Chen Ying Zhang Yuehong Sun Shan Yang Chang Chen
author_sort	Bing Liu
title	CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
title_short	CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
title_full	CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
title_fullStr	CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
title_full_unstemmed	CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>
title_sort	csra regulates swarming motility and carbohydrate and amino acid metabolism in <i>vibrio alginolyticus</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3ad913496ad64df1b293b24369d5326c
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CsrA Regulates Swarming Motility and Carbohydrate and Amino Acid Metabolism in <i>Vibrio alginolyticus</i>

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