Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat

Abstract The lymphatic system plays a key role in tissue fluid homeostasis, immune cell trafficking, and fat absorption. We previously reported a bacterial artificial chromosome (BAC)-based lymphatic reporter mouse, where EGFP is expressed under the regulation of the Prox1 promoter. This reporter li...

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Autores principales:	Eunson Jung, Daniel Gardner, Dongwon Choi, Eunkyung Park, Young Jin Seong, Sara Yang, Jorge Castorena-Gonzalez, Antoine Louveau, Zhao Zhou, Gene K. Lee, David P. Perrault, Sunju Lee, Maxwell Johnson, George Daghlian, Maria Lee, Yeo Jin Hong, Yukinari Kato, Jonathan Kipnis, Michael J. Davis, Alex K. Wong, Young-Kwon Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3adf856260b840d4ad1dd57843cdb6d1
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spelling	oai:doaj.org-article:3adf856260b840d4ad1dd57843cdb6d12021-12-02T16:07:06ZDevelopment and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat10.1038/s41598-017-06031-32045-2322https://doaj.org/article/3adf856260b840d4ad1dd57843cdb6d12017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06031-3https://doaj.org/toc/2045-2322Abstract The lymphatic system plays a key role in tissue fluid homeostasis, immune cell trafficking, and fat absorption. We previously reported a bacterial artificial chromosome (BAC)-based lymphatic reporter mouse, where EGFP is expressed under the regulation of the Prox1 promoter. This reporter line has been widely used to conveniently visualize lymphatic vessels and other Prox1-expressing tissues such as Schlemm’s canal. However, mice have a number of experimental limitations due to small body size. By comparison, laboratory rats are larger in size and more closely model the metabolic, physiological, and surgical aspects of humans. Here, we report development of a novel lymphatic reporter rat using the mouse Prox1-EGFP BAC. Despite the species mismatch, the mouse Prox1-EGFP BAC enabled a reliable expression of EGFP in Prox1-expressing cells of the transgenic rats and allowed a convenient visualization of all lymphatic vessels, including those in the central nervous system, and Schlemm’s canal. To demonstrate the utility of this new reporter rat, we studied the contractile properties and valvular functions of mesenteric lymphatics, developed a surgical model for vascularized lymph node transplantation, and confirmed Prox1 expression in venous valves. Together, Prox1-EGFP rat model will contribute to the advancement of lymphatic research as a valuable experimental resource.Eunson JungDaniel GardnerDongwon ChoiEunkyung ParkYoung Jin SeongSara YangJorge Castorena-GonzalezAntoine LouveauZhao ZhouGene K. LeeDavid P. PerraultSunju LeeMaxwell JohnsonGeorge DaghlianMaria LeeYeo Jin HongYukinari KatoJonathan KipnisMichael J. DavisAlex K. WongYoung-Kwon HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eunson Jung Daniel Gardner Dongwon Choi Eunkyung Park Young Jin Seong Sara Yang Jorge Castorena-Gonzalez Antoine Louveau Zhao Zhou Gene K. Lee David P. Perrault Sunju Lee Maxwell Johnson George Daghlian Maria Lee Yeo Jin Hong Yukinari Kato Jonathan Kipnis Michael J. Davis Alex K. Wong Young-Kwon Hong Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
description	Abstract The lymphatic system plays a key role in tissue fluid homeostasis, immune cell trafficking, and fat absorption. We previously reported a bacterial artificial chromosome (BAC)-based lymphatic reporter mouse, where EGFP is expressed under the regulation of the Prox1 promoter. This reporter line has been widely used to conveniently visualize lymphatic vessels and other Prox1-expressing tissues such as Schlemm’s canal. However, mice have a number of experimental limitations due to small body size. By comparison, laboratory rats are larger in size and more closely model the metabolic, physiological, and surgical aspects of humans. Here, we report development of a novel lymphatic reporter rat using the mouse Prox1-EGFP BAC. Despite the species mismatch, the mouse Prox1-EGFP BAC enabled a reliable expression of EGFP in Prox1-expressing cells of the transgenic rats and allowed a convenient visualization of all lymphatic vessels, including those in the central nervous system, and Schlemm’s canal. To demonstrate the utility of this new reporter rat, we studied the contractile properties and valvular functions of mesenteric lymphatics, developed a surgical model for vascularized lymph node transplantation, and confirmed Prox1 expression in venous valves. Together, Prox1-EGFP rat model will contribute to the advancement of lymphatic research as a valuable experimental resource.
format	article
author	Eunson Jung Daniel Gardner Dongwon Choi Eunkyung Park Young Jin Seong Sara Yang Jorge Castorena-Gonzalez Antoine Louveau Zhao Zhou Gene K. Lee David P. Perrault Sunju Lee Maxwell Johnson George Daghlian Maria Lee Yeo Jin Hong Yukinari Kato Jonathan Kipnis Michael J. Davis Alex K. Wong Young-Kwon Hong
author_facet	Eunson Jung Daniel Gardner Dongwon Choi Eunkyung Park Young Jin Seong Sara Yang Jorge Castorena-Gonzalez Antoine Louveau Zhao Zhou Gene K. Lee David P. Perrault Sunju Lee Maxwell Johnson George Daghlian Maria Lee Yeo Jin Hong Yukinari Kato Jonathan Kipnis Michael J. Davis Alex K. Wong Young-Kwon Hong
author_sort	Eunson Jung
title	Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
title_short	Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
title_full	Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
title_fullStr	Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
title_full_unstemmed	Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat
title_sort	development and characterization of a novel prox1-egfp lymphatic and schlemm’s canal reporter rat
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3adf856260b840d4ad1dd57843cdb6d1
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Development and Characterization of A Novel Prox1-EGFP Lymphatic and Schlemm’s Canal Reporter Rat

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