Correlative nanoscale 3D imaging of structure and composition in extended objects.

Structure and composition at the nanoscale determine the behavior of biological systems and engineered materials. The drive to understand and control this behavior has placed strong demands on developing methods for high resolution imaging. In general, the improvement of three-dimensional (3D) resol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Feng Xu, Lukas Helfen, Heikki Suhonen, Dan Elgrabli, Sam Bayat, Péter Reischig, Tilo Baumbach, Peter Cloetens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ae240cb6fe940cb94e8bf578d2b672f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3ae240cb6fe940cb94e8bf578d2b672f
record_format	dspace
spelling	oai:doaj.org-article:3ae240cb6fe940cb94e8bf578d2b672f2021-11-18T08:08:14ZCorrelative nanoscale 3D imaging of structure and composition in extended objects.1932-620310.1371/journal.pone.0050124https://doaj.org/article/3ae240cb6fe940cb94e8bf578d2b672f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23185554/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Structure and composition at the nanoscale determine the behavior of biological systems and engineered materials. The drive to understand and control this behavior has placed strong demands on developing methods for high resolution imaging. In general, the improvement of three-dimensional (3D) resolution is accomplished by tightening constraints: reduced manageable specimen sizes, decreasing analyzable volumes, degrading contrasts, and increasing sample preparation efforts. Aiming to overcome these limitations, we present a non-destructive and multiple-contrast imaging technique, using principles of X-ray laminography, thus generalizing tomography towards laterally extended objects. We retain advantages that are usually restricted to 2D microscopic imaging, such as scanning of large areas and subsequent zooming-in towards a region of interest at the highest possible resolution. Our technique permits correlating the 3D structure and the elemental distribution yielding a high sensitivity to variations of the electron density via coherent imaging and to local trace element quantification through X-ray fluorescence. We demonstrate the method by imaging a lithographic nanostructure and an aluminum alloy. Analyzing a biological system, we visualize in lung tissue the subcellular response to toxic stress after exposure to nanotubes. We show that most of the nanotubes are trapped inside alveolar macrophages, while a small portion of the nanotubes has crossed the barrier to the cellular space of the alveolar wall. In general, our method is non-destructive and can be combined with different sample environmental or loading conditions. We therefore anticipate that correlative X-ray nano-laminography will enable a variety of in situ and in operando 3D studies.Feng XuLukas HelfenHeikki SuhonenDan ElgrabliSam BayatPéter ReischigTilo BaumbachPeter CloetensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50124 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Feng Xu Lukas Helfen Heikki Suhonen Dan Elgrabli Sam Bayat Péter Reischig Tilo Baumbach Peter Cloetens Correlative nanoscale 3D imaging of structure and composition in extended objects.
description	Structure and composition at the nanoscale determine the behavior of biological systems and engineered materials. The drive to understand and control this behavior has placed strong demands on developing methods for high resolution imaging. In general, the improvement of three-dimensional (3D) resolution is accomplished by tightening constraints: reduced manageable specimen sizes, decreasing analyzable volumes, degrading contrasts, and increasing sample preparation efforts. Aiming to overcome these limitations, we present a non-destructive and multiple-contrast imaging technique, using principles of X-ray laminography, thus generalizing tomography towards laterally extended objects. We retain advantages that are usually restricted to 2D microscopic imaging, such as scanning of large areas and subsequent zooming-in towards a region of interest at the highest possible resolution. Our technique permits correlating the 3D structure and the elemental distribution yielding a high sensitivity to variations of the electron density via coherent imaging and to local trace element quantification through X-ray fluorescence. We demonstrate the method by imaging a lithographic nanostructure and an aluminum alloy. Analyzing a biological system, we visualize in lung tissue the subcellular response to toxic stress after exposure to nanotubes. We show that most of the nanotubes are trapped inside alveolar macrophages, while a small portion of the nanotubes has crossed the barrier to the cellular space of the alveolar wall. In general, our method is non-destructive and can be combined with different sample environmental or loading conditions. We therefore anticipate that correlative X-ray nano-laminography will enable a variety of in situ and in operando 3D studies.
format	article
author	Feng Xu Lukas Helfen Heikki Suhonen Dan Elgrabli Sam Bayat Péter Reischig Tilo Baumbach Peter Cloetens
author_facet	Feng Xu Lukas Helfen Heikki Suhonen Dan Elgrabli Sam Bayat Péter Reischig Tilo Baumbach Peter Cloetens
author_sort	Feng Xu
title	Correlative nanoscale 3D imaging of structure and composition in extended objects.
title_short	Correlative nanoscale 3D imaging of structure and composition in extended objects.
title_full	Correlative nanoscale 3D imaging of structure and composition in extended objects.
title_fullStr	Correlative nanoscale 3D imaging of structure and composition in extended objects.
title_full_unstemmed	Correlative nanoscale 3D imaging of structure and composition in extended objects.
title_sort	correlative nanoscale 3d imaging of structure and composition in extended objects.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/3ae240cb6fe940cb94e8bf578d2b672f
work_keys_str_mv	AT fengxu correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT lukashelfen correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT heikkisuhonen correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT danelgrabli correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT sambayat correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT peterreischig correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT tilobaumbach correlativenanoscale3dimagingofstructureandcompositioninextendedobjects AT petercloetens correlativenanoscale3dimagingofstructureandcompositioninextendedobjects
_version_	1718422161678401536

Correlative nanoscale 3D imaging of structure and composition in extended objects.

Ejemplares similares