Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta

Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequent...

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Autores principales:	Katon Waskito Aji, Tuty Arisuryanti
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	coi cryptic species dna barcoding mudskipper Agriculture (General) S1-972 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/3aeacdca84c64dfd806cdb90350b9008
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spelling	oai:doaj.org-article:3aeacdca84c64dfd806cdb90350b90082021-11-08T08:40:57ZMolecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta2540-95732540-958110.22146/jtbb.66391https://doaj.org/article/3aeacdca84c64dfd806cdb90350b90082021-10-01T00:00:00Zhttps://jurnal.ugm.ac.id/jtbb/article/view/66391https://doaj.org/toc/2540-9573https://doaj.org/toc/2540-9581Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species.Katon Waskito AjiTuty ArisuryantiUniversitas Gadjah Madaarticlecoicryptic speciesdna barcodingmudskipperAgriculture (General)S1-972Plant cultureSB1-1110ENJournal of Tropical Biodiversity and Biotechnology, Vol 6, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coi cryptic species dna barcoding mudskipper Agriculture (General) S1-972 Plant culture SB1-1110
spellingShingle	coi cryptic species dna barcoding mudskipper Agriculture (General) S1-972 Plant culture SB1-1110 Katon Waskito Aji Tuty Arisuryanti Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
description	Mudskipper fish is amphibious fish belonging to the family Gobiidae. Coastal communities widely consume mudskipper to meet their animal protein needs. Mudskipper is primarily cryptic species that are morphologically difficult to identify and distinguish from other mudskipper fish species. Consequently, it can be confused with the naming of mudskipper fish species and can affect the conservation efforts of the fish in their habitat. One of the molecular approaches that can be used to identify the fish species quickly and accurately is DNA barcoding using the COI mitochondrial gene. However, the research on the identification of mudskipper fish in Indonesia is still very limited. Therefore, this study aimed to identify 26 mudskipper fish from Baros Beach, Bantul, Yogyakarta, using COI mitochondrial gene as a molecular marker for DNA barcoding. The method used in this study was a PCR method with universal primers, FishF2 and FishR2. The data obtained were then analyzed using GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, and BEAST. The analysis was conducted to obtain similarity, genetic distance and reconstruct a phylogenetic tree. The result revealed that all 26 samples of mudskippers collected from Baros Beach were identified in one genus, namely Periophthalmus, and consisted of 3 species, namely P. kalolo (16 samples), P. argentilineatus (9 samples), and P. novemradiatus (1 sample). Furthermore, this study also discovered a suspected cryptic species in P. argentilineatus with a genetic distance of 5.46-5.96% between clade E, F compared with clade G. Further morphological studies are needed to confirm the species status of these three clades before solidly proclaim that they are cryptic species.
format	article
author	Katon Waskito Aji Tuty Arisuryanti
author_facet	Katon Waskito Aji Tuty Arisuryanti
author_sort	Katon Waskito Aji
title	Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
title_short	Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
title_full	Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
title_fullStr	Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
title_full_unstemmed	Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta
title_sort	molecular identification of mudskipper fish (<i>periophthalmus</i> spp.) from baros beach, bantul, yogyakarta
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/3aeacdca84c64dfd806cdb90350b9008
work_keys_str_mv	AT katonwaskitoaji molecularidentificationofmudskipperfishiperiophthalmusisppfrombarosbeachbantulyogyakarta AT tutyarisuryanti molecularidentificationofmudskipperfishiperiophthalmusisppfrombarosbeachbantulyogyakarta
_version_	1718442703244492800

Molecular Identification of Mudskipper Fish (<i>Periophthalmus</i> spp.) from Baros Beach, Bantul, Yogyakarta

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