mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells

Abstract The potential applications of human pluripotent stem cells, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells in cell therapy and regenerative medicine have been widely studied. The precise definition of pluripotent stem cell status during culture using biomarkers is essen...

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Autores principales:	Shuuji Mawaribuchi, Yasuhiko Aiki, Nozomi Ikeda, Yuzuru Ito
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b00623ef8d545b28441bb299f7cad47
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spelling	oai:doaj.org-article:3b00623ef8d545b28441bb299f7cad472021-12-02T16:07:53ZmRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells10.1038/s41598-019-48447-z2045-2322https://doaj.org/article/3b00623ef8d545b28441bb299f7cad472019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48447-zhttps://doaj.org/toc/2045-2322Abstract The potential applications of human pluripotent stem cells, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells in cell therapy and regenerative medicine have been widely studied. The precise definition of pluripotent stem cell status during culture using biomarkers is essential for basic research and regenerative medicine. Culture conditions, including extracellular matrices, influence the balance between self-renewal and differentiation. Accordingly, to explore biomarkers for defining and monitoring the pluripotent substates during culture, we established different substates in H9 human ES cells by changing the extracellular matrix from vitronectin to Matrigel. The substate was characterised by low and high expression of the pluripotency marker R-10G epitope and the mesenchymal marker vimentin, respectively. Immunohistochemistry, induction of the three germ layers, and exhaustive expression analysis showed that the substate was ectoderm-biased, tended to differentiate into nerves, but retained the potential to differentiate into the three germ layers. Further integrated analyses of mRNA and miRNA microarrays and qPCR analysis showed that nine genes (COL9A2, DGKI, GBX2, KIF26B, MARCH1, PLXNA4, SLC24A4, TLR4, and ZHX3) were upregulated in the ectoderm-biased cells as ectoderm-biased biomarker candidates in pluripotent stem cells. Our findings provide important insights into ectoderm-biased substates of human pluripotent stem cells in the fields of basic research and regenerative medicine.Shuuji MawaribuchiYasuhiko AikiNozomi IkedaYuzuru ItoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuuji Mawaribuchi Yasuhiko Aiki Nozomi Ikeda Yuzuru Ito mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
description	Abstract The potential applications of human pluripotent stem cells, embryonic stem (ES) cells, and induced pluripotent stem (iPS) cells in cell therapy and regenerative medicine have been widely studied. The precise definition of pluripotent stem cell status during culture using biomarkers is essential for basic research and regenerative medicine. Culture conditions, including extracellular matrices, influence the balance between self-renewal and differentiation. Accordingly, to explore biomarkers for defining and monitoring the pluripotent substates during culture, we established different substates in H9 human ES cells by changing the extracellular matrix from vitronectin to Matrigel. The substate was characterised by low and high expression of the pluripotency marker R-10G epitope and the mesenchymal marker vimentin, respectively. Immunohistochemistry, induction of the three germ layers, and exhaustive expression analysis showed that the substate was ectoderm-biased, tended to differentiate into nerves, but retained the potential to differentiate into the three germ layers. Further integrated analyses of mRNA and miRNA microarrays and qPCR analysis showed that nine genes (COL9A2, DGKI, GBX2, KIF26B, MARCH1, PLXNA4, SLC24A4, TLR4, and ZHX3) were upregulated in the ectoderm-biased cells as ectoderm-biased biomarker candidates in pluripotent stem cells. Our findings provide important insights into ectoderm-biased substates of human pluripotent stem cells in the fields of basic research and regenerative medicine.
format	article
author	Shuuji Mawaribuchi Yasuhiko Aiki Nozomi Ikeda Yuzuru Ito
author_facet	Shuuji Mawaribuchi Yasuhiko Aiki Nozomi Ikeda Yuzuru Ito
author_sort	Shuuji Mawaribuchi
title	mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
title_short	mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
title_full	mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
title_fullStr	mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
title_full_unstemmed	mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells
title_sort	mrna and mirna expression profiles in an ectoderm-biased substate of human pluripotent stem cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/3b00623ef8d545b28441bb299f7cad47
work_keys_str_mv	AT shuujimawaribuchi mrnaandmirnaexpressionprofilesinanectodermbiasedsubstateofhumanpluripotentstemcells AT yasuhikoaiki mrnaandmirnaexpressionprofilesinanectodermbiasedsubstateofhumanpluripotentstemcells AT nozomiikeda mrnaandmirnaexpressionprofilesinanectodermbiasedsubstateofhumanpluripotentstemcells AT yuzuruito mrnaandmirnaexpressionprofilesinanectodermbiasedsubstateofhumanpluripotentstemcells
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mRNA and miRNA expression profiles in an ectoderm-biased substate of human pluripotent stem cells

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