2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.

Analysis of gene function in Plasmodium falciparum, the most important human malaria parasite, is restricted by the lack of robust and simple reverse genetic tools. Approaches to manipulate protein levels post-translationally are powerful tools to study protein-off effects especially in the haploid...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrea Kreidenweiss, Annika V Hopkins, Benjamin Mordmüller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b007483133b4e5281463b779559f608
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3b007483133b4e5281463b779559f608
record_format	dspace
spelling	oai:doaj.org-article:3b007483133b4e5281463b779559f6082021-11-18T08:46:56Z2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.1932-620310.1371/journal.pone.0078661https://doaj.org/article/3b007483133b4e5281463b779559f6082013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24236031/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Analysis of gene function in Plasmodium falciparum, the most important human malaria parasite, is restricted by the lack of robust and simple reverse genetic tools. Approaches to manipulate protein levels post-translationally are powerful tools to study protein-off effects especially in the haploid malaria parasite where genetic knockouts of essential genes are lethal. We investigated if the auxin-inducible degron system is functional in P. falciparum and found that degron-tagged yellow fluorescent protein levels were efficiently reduced upon addition of auxin which otherwise had no effect on parasite viability. The genetic components required in this conditional approach were co-expressed in P. falciparum by applying the small peptide 2A. 2A is a self-processing peptide from Foot-And-Mouth Disease virus that allows the whole conditional system to be accommodated on a single plasmid vector and ensures stoichiometric expression levels.Andrea KreidenweissAnnika V HopkinsBenjamin MordmüllerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78661 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Kreidenweiss Annika V Hopkins Benjamin Mordmüller 2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
description	Analysis of gene function in Plasmodium falciparum, the most important human malaria parasite, is restricted by the lack of robust and simple reverse genetic tools. Approaches to manipulate protein levels post-translationally are powerful tools to study protein-off effects especially in the haploid malaria parasite where genetic knockouts of essential genes are lethal. We investigated if the auxin-inducible degron system is functional in P. falciparum and found that degron-tagged yellow fluorescent protein levels were efficiently reduced upon addition of auxin which otherwise had no effect on parasite viability. The genetic components required in this conditional approach were co-expressed in P. falciparum by applying the small peptide 2A. 2A is a self-processing peptide from Foot-And-Mouth Disease virus that allows the whole conditional system to be accommodated on a single plasmid vector and ensures stoichiometric expression levels.
format	article
author	Andrea Kreidenweiss Annika V Hopkins Benjamin Mordmüller
author_facet	Andrea Kreidenweiss Annika V Hopkins Benjamin Mordmüller
author_sort	Andrea Kreidenweiss
title	2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
title_short	2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
title_full	2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
title_fullStr	2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
title_full_unstemmed	2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.
title_sort	2a and the auxin-based degron system facilitate control of protein levels in plasmodium falciparum.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3b007483133b4e5281463b779559f608
work_keys_str_mv	AT andreakreidenweiss 2aandtheauxinbaseddegronsystemfacilitatecontrolofproteinlevelsinplasmodiumfalciparum AT annikavhopkins 2aandtheauxinbaseddegronsystemfacilitatecontrolofproteinlevelsinplasmodiumfalciparum AT benjaminmordmuller 2aandtheauxinbaseddegronsystemfacilitatecontrolofproteinlevelsinplasmodiumfalciparum
_version_	1718421315773267968

2A and the auxin-based degron system facilitate control of protein levels in Plasmodium falciparum.

Ejemplares similares