Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi

In this paper, we have presented the method of green synthesis of ZnO and Ag-NPs using the callus extract (CE) of medicinally important Cannabis sativa. The synthesis of nanoparticles (NPs) was confirmed by UV-Vis spectroscopy, while as far as the size and shape of the NPs were concerned, they were...

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Autores principales:	Zaka Mehreen, Hashmi Syed Salman, Siddiqui Moiz A., Rahman Lubna, Mushtaq Sadaf, Ali Haider, Hano Christophe, Abbasi Bilal Haider
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	cannabis sativa callus extract green synthesis ag-nps zno-nps Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3b027174e13e40958f3f5f87064fda75
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spelling	oai:doaj.org-article:3b027174e13e40958f3f5f87064fda752021-12-05T14:10:50ZCallus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi2191-955010.1515/gps-2021-0057https://doaj.org/article/3b027174e13e40958f3f5f87064fda752021-09-01T00:00:00Zhttps://doi.org/10.1515/gps-2021-0057https://doaj.org/toc/2191-9550In this paper, we have presented the method of green synthesis of ZnO and Ag-NPs using the callus extract (CE) of medicinally important Cannabis sativa. The synthesis of nanoparticles (NPs) was confirmed by UV-Vis spectroscopy, while as far as the size and shape of the NPs were concerned, they were validated using the techniques of X-ray diffraction and scanning electron microscopy, respectively. The energy dispersive X-ray analysis graph confirmed the constitution of elements along with the surface chemical state of NPs. Fourier transform-infrared spectroscopy was utilized for the confirmation of biomolecules capping the NPs. In order to test the application of these biosynthesized NPs on biological entities, four bacterial strains, including Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa, were used. On the other hand, five fungal strains, namely Mucor, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, and Fusarium solani, were utilized for antifungal assay. Cytotoxicity assay was also performed using the HepG2 cell line. The results showed considerable antibacterial and antifungal activities. It also showed better cytotoxicity values as compared to the control.Zaka MehreenHashmi Syed SalmanSiddiqui Moiz A.Rahman LubnaMushtaq SadafAli HaiderHano ChristopheAbbasi Bilal HaiderDe Gruyterarticlecannabis sativacallus extractgreen synthesisag-npszno-npsChemistryQD1-999ENGreen Processing and Synthesis, Vol 10, Iss 1, Pp 569-584 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cannabis sativa callus extract green synthesis ag-nps zno-nps Chemistry QD1-999
spellingShingle	cannabis sativa callus extract green synthesis ag-nps zno-nps Chemistry QD1-999 Zaka Mehreen Hashmi Syed Salman Siddiqui Moiz A. Rahman Lubna Mushtaq Sadaf Ali Haider Hano Christophe Abbasi Bilal Haider Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
description	In this paper, we have presented the method of green synthesis of ZnO and Ag-NPs using the callus extract (CE) of medicinally important Cannabis sativa. The synthesis of nanoparticles (NPs) was confirmed by UV-Vis spectroscopy, while as far as the size and shape of the NPs were concerned, they were validated using the techniques of X-ray diffraction and scanning electron microscopy, respectively. The energy dispersive X-ray analysis graph confirmed the constitution of elements along with the surface chemical state of NPs. Fourier transform-infrared spectroscopy was utilized for the confirmation of biomolecules capping the NPs. In order to test the application of these biosynthesized NPs on biological entities, four bacterial strains, including Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, and Pseudomonas aeruginosa, were used. On the other hand, five fungal strains, namely Mucor, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, and Fusarium solani, were utilized for antifungal assay. Cytotoxicity assay was also performed using the HepG2 cell line. The results showed considerable antibacterial and antifungal activities. It also showed better cytotoxicity values as compared to the control.
format	article
author	Zaka Mehreen Hashmi Syed Salman Siddiqui Moiz A. Rahman Lubna Mushtaq Sadaf Ali Haider Hano Christophe Abbasi Bilal Haider
author_facet	Zaka Mehreen Hashmi Syed Salman Siddiqui Moiz A. Rahman Lubna Mushtaq Sadaf Ali Haider Hano Christophe Abbasi Bilal Haider
author_sort	Zaka Mehreen
title	Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
title_short	Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
title_full	Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
title_fullStr	Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
title_full_unstemmed	Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
title_sort	callus-mediated biosynthesis of ag and zno nanoparticles using aqueous callus extract of cannabis sativa: their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/3b027174e13e40958f3f5f87064fda75
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Callus-mediated biosynthesis of Ag and ZnO nanoparticles using aqueous callus extract of Cannabis sativa: Their cytotoxic potential and clinical potential against human pathogenic bacteria and fungi

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