Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.

Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898...

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Autores principales:	Leon E Hugo, James Monkman, Keyur A Dave, Leesa F Wockner, Geoff W Birrell, Emma L Norris, Vivian J Kienzle, Maggy T Sikulu, Peter A Ryan, Jeffery J Gorman, Brian H Kay
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b06e435416340f980ac9b25d9889a35
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spelling	oai:doaj.org-article:3b06e435416340f980ac9b25d9889a352021-11-18T07:53:58ZProteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.1932-620310.1371/journal.pone.0058656https://doaj.org/article/3b06e435416340f980ac9b25d9889a352013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23536806/?tool=EBIhttps://doaj.org/toc/1932-6203Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.Leon E HugoJames MonkmanKeyur A DaveLeesa F WocknerGeoff W BirrellEmma L NorrisVivian J KienzleMaggy T SikuluPeter A RyanJeffery J GormanBrian H KayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58656 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leon E Hugo James Monkman Keyur A Dave Leesa F Wockner Geoff W Birrell Emma L Norris Vivian J Kienzle Maggy T Sikulu Peter A Ryan Jeffery J Gorman Brian H Kay Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
description	Biomarkers of the age of mosquitoes are required to determine the risk of transmission of various pathogens as each pathogen undergoes a period of extrinsic incubation in the mosquito host. Using the 2-D Difference Gel Electrophoresis (2-D DIGE) procedure, we investigated the abundance of up to 898 proteins from the Yellow Fever and dengue virus vector, Aedes aegypti, during ageing. By applying a mixed-effects model of protein expression, we identified five common patterns of abundance change during ageing and demonstrated an age-related decrease in variance for four of these. This supported a search for specific proteins with abundance changes that remain tightly associated with ageing for use as ageing biomarkers. Using MALDI-TOF/TOF mass spectrometry we identified ten candidate proteins that satisfied strict biomarker discovery criteria (identified in two out of three multivariate analysis procedures and in two cohorts of mosquitoes). We validated the abundances of the four most suitable candidates (Actin depolymerising factor; ADF, Eukaryotic initiation factor 5A; eIF5A, insect cuticle protein Q17LN8, and Anterior fat body protein; AFP) using semi-quantitative Western analysis of individual mosquitoes of six ages. The redox-response protein Manganese superoxide dismutase (SOD2) and electron shuttling protein Electron transfer oxidoreductase (ETO) were subject to post-translational modifications affecting their charge states with potential effects on function. For the four candidates we show remarkably consistent decreases in abundance during ageing, validating initial selections. In particular, the abundance of AFP is an ideal biomarker candidate for whether a female mosquito has lived long enough to be capable of dengue virus transmission. We have demonstrated proteins to be a suitable class of ageing biomarkers in mosquitoes and have identified candidates for epidemiological studies of dengue and the evaluation of new disease reduction projects targeting mosquito longevity.
format	article
author	Leon E Hugo James Monkman Keyur A Dave Leesa F Wockner Geoff W Birrell Emma L Norris Vivian J Kienzle Maggy T Sikulu Peter A Ryan Jeffery J Gorman Brian H Kay
author_facet	Leon E Hugo James Monkman Keyur A Dave Leesa F Wockner Geoff W Birrell Emma L Norris Vivian J Kienzle Maggy T Sikulu Peter A Ryan Jeffery J Gorman Brian H Kay
author_sort	Leon E Hugo
title	Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
title_short	Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
title_full	Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
title_fullStr	Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
title_full_unstemmed	Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.
title_sort	proteomic biomarkers for ageing the mosquito aedes aegypti to determine risk of pathogen transmission.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3b06e435416340f980ac9b25d9889a35
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Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.

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