The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes

Abstract Phosphoprotein phosphatase (PPP) enzymes are ubiquitous proteins involved in cellular signaling pathways and other functions. Here we have traced the origin of the PPP sequences of Eukaryotes and their radiation. Using a bacterial PPP Hidden Markov Model (HMM) we uncovered “BacterialPPP-Lik...

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Autores principales:	David Kerk, Jordan F. Mattice, Mario E. Valdés-Tresanco, Sergei Yu Noskov, Kenneth K.-S. Ng, Greg B. Moorhead
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b06f50c4dba434391b0f94c6280040b
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spelling	oai:doaj.org-article:3b06f50c4dba434391b0f94c6280040b2021-12-02T18:18:51ZThe origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes10.1038/s41598-021-93206-82045-2322https://doaj.org/article/3b06f50c4dba434391b0f94c6280040b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93206-8https://doaj.org/toc/2045-2322Abstract Phosphoprotein phosphatase (PPP) enzymes are ubiquitous proteins involved in cellular signaling pathways and other functions. Here we have traced the origin of the PPP sequences of Eukaryotes and their radiation. Using a bacterial PPP Hidden Markov Model (HMM) we uncovered “BacterialPPP-Like” sequences in Archaea. A HMM derived from eukaryotic PPP enzymes revealed additional, unique sequences in Archaea and Bacteria that were more like the eukaryotic PPP enzymes then the bacterial PPPs. These sequences formed the basis of phylogenetic tree inference and sequence structural analysis allowing the history of these sequence types to be elucidated. Our phylogenetic tree data strongly suggest that eukaryotic PPPs ultimately arose from ancestors in the Asgard archaea. We have clarified the radiation of PPPs within Eukaryotes, substantially expanding the range of known organisms with PPP subtypes (Bsu1, PP7, PPEF/RdgC) previously thought to have a more restricted distribution. Surprisingly, sequences from the Methanosarcinaceae (Euryarchaeota) form a strongly supported sister group to eukaryotic PPPs in our phylogenetic analysis. This strongly suggests an intimate association between an Asgard ancestor and that of the Methanosarcinaceae. This is highly reminiscent of the syntrophic association recently demonstrated between the cultured Lokiarchaeal species Prometheoarchaeum and a methanogenic bacterial species.David KerkJordan F. MatticeMario E. Valdés-TresancoSergei Yu NoskovKenneth K.-S. NgGreg B. MoorheadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Kerk Jordan F. Mattice Mario E. Valdés-Tresanco Sergei Yu Noskov Kenneth K.-S. Ng Greg B. Moorhead The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
description	Abstract Phosphoprotein phosphatase (PPP) enzymes are ubiquitous proteins involved in cellular signaling pathways and other functions. Here we have traced the origin of the PPP sequences of Eukaryotes and their radiation. Using a bacterial PPP Hidden Markov Model (HMM) we uncovered “BacterialPPP-Like” sequences in Archaea. A HMM derived from eukaryotic PPP enzymes revealed additional, unique sequences in Archaea and Bacteria that were more like the eukaryotic PPP enzymes then the bacterial PPPs. These sequences formed the basis of phylogenetic tree inference and sequence structural analysis allowing the history of these sequence types to be elucidated. Our phylogenetic tree data strongly suggest that eukaryotic PPPs ultimately arose from ancestors in the Asgard archaea. We have clarified the radiation of PPPs within Eukaryotes, substantially expanding the range of known organisms with PPP subtypes (Bsu1, PP7, PPEF/RdgC) previously thought to have a more restricted distribution. Surprisingly, sequences from the Methanosarcinaceae (Euryarchaeota) form a strongly supported sister group to eukaryotic PPPs in our phylogenetic analysis. This strongly suggests an intimate association between an Asgard ancestor and that of the Methanosarcinaceae. This is highly reminiscent of the syntrophic association recently demonstrated between the cultured Lokiarchaeal species Prometheoarchaeum and a methanogenic bacterial species.
format	article
author	David Kerk Jordan F. Mattice Mario E. Valdés-Tresanco Sergei Yu Noskov Kenneth K.-S. Ng Greg B. Moorhead
author_facet	David Kerk Jordan F. Mattice Mario E. Valdés-Tresanco Sergei Yu Noskov Kenneth K.-S. Ng Greg B. Moorhead
author_sort	David Kerk
title	The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
title_short	The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
title_full	The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
title_fullStr	The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
title_full_unstemmed	The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes
title_sort	origin and radiation of the phosphoprotein phosphatase (ppp) enzymes of eukaryotes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b06f50c4dba434391b0f94c6280040b
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The origin and radiation of the phosphoprotein phosphatase (PPP) enzymes of Eukaryotes

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