The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

Abstract The extent of regeneration following biomaterial implantation is dependent on the microenvironment surrounding the implant. Since implant composition can have a profound effect on inflammation, it is essential to understand this process as a non-resolving inflammatory response can lead to f...

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Autores principales:	Filipa Lebre, Rukmani Sridharan, Michael J. Sawkins, Daniel J. Kelly, Fergal J. O’Brien, Ed C. Lavelle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b130aa0bb9d4063b965d2084cb2518c
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spelling	oai:doaj.org-article:3b130aa0bb9d4063b965d2084cb2518c2021-12-02T11:52:41ZThe shape and size of hydroxyapatite particles dictate inflammatory responses following implantation10.1038/s41598-017-03086-02045-2322https://doaj.org/article/3b130aa0bb9d4063b965d2084cb2518c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03086-0https://doaj.org/toc/2045-2322Abstract The extent of regeneration following biomaterial implantation is dependent on the microenvironment surrounding the implant. Since implant composition can have a profound effect on inflammation, it is essential to understand this process as a non-resolving inflammatory response can lead to fibrous encapsulation and insufficient integration. Incorporation of particulates into implants confers structural and functional benefits, thus optimizing particulate characteristics to enhance immune mediated efficacy is important. We investigated the relationship between the nature of hydroxyapatite (HA) particles and the innate immune response, focusing on how particle size (0.1 µm, 5 µm, 20 µm, 100 µm) and morphology (needle-shaped/spherical; smooth/rough surface) modulates inflammatory responses. We observed a shape and size-dependent activation of the NLRP3 inflammasome and IL-1β secretion; while needle-shaped and smaller HA particles significantly enhanced cytokine secretion, larger particles did not. Moreover, HA particle characteristics profoundly influenced patterns of innate immune cell recruitment and cytokine production following injection. While small, needle-shaped particles induced a strong inflammatory response, this was not observed with smooth, spherical particles of comparable size or with larger particles. These findings indicate that hydroxyapatite particle characteristics dictate immune cell recruitment and the ensuing inflammatory response, providing an opportunity to tailor HA particle characteristics to regulate immune responses induced after biomaterial implantation.Filipa LebreRukmani SridharanMichael J. SawkinsDaniel J. KellyFergal J. O’BrienEd C. LavelleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Filipa Lebre Rukmani Sridharan Michael J. Sawkins Daniel J. Kelly Fergal J. O’Brien Ed C. Lavelle The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
description	Abstract The extent of regeneration following biomaterial implantation is dependent on the microenvironment surrounding the implant. Since implant composition can have a profound effect on inflammation, it is essential to understand this process as a non-resolving inflammatory response can lead to fibrous encapsulation and insufficient integration. Incorporation of particulates into implants confers structural and functional benefits, thus optimizing particulate characteristics to enhance immune mediated efficacy is important. We investigated the relationship between the nature of hydroxyapatite (HA) particles and the innate immune response, focusing on how particle size (0.1 µm, 5 µm, 20 µm, 100 µm) and morphology (needle-shaped/spherical; smooth/rough surface) modulates inflammatory responses. We observed a shape and size-dependent activation of the NLRP3 inflammasome and IL-1β secretion; while needle-shaped and smaller HA particles significantly enhanced cytokine secretion, larger particles did not. Moreover, HA particle characteristics profoundly influenced patterns of innate immune cell recruitment and cytokine production following injection. While small, needle-shaped particles induced a strong inflammatory response, this was not observed with smooth, spherical particles of comparable size or with larger particles. These findings indicate that hydroxyapatite particle characteristics dictate immune cell recruitment and the ensuing inflammatory response, providing an opportunity to tailor HA particle characteristics to regulate immune responses induced after biomaterial implantation.
format	article
author	Filipa Lebre Rukmani Sridharan Michael J. Sawkins Daniel J. Kelly Fergal J. O’Brien Ed C. Lavelle
author_facet	Filipa Lebre Rukmani Sridharan Michael J. Sawkins Daniel J. Kelly Fergal J. O’Brien Ed C. Lavelle
author_sort	Filipa Lebre
title	The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
title_short	The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
title_full	The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
title_fullStr	The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
title_full_unstemmed	The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
title_sort	shape and size of hydroxyapatite particles dictate inflammatory responses following implantation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3b130aa0bb9d4063b965d2084cb2518c
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The shape and size of hydroxyapatite particles dictate inflammatory responses following implantation

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