Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production

Mycoplasma contamination remains a major concern in the biopharmaceutical industry especially in tissue culture based viral vaccine and its presence and/or its endotoxin-like metabolites in the final products can result in pyrogenic responses ranging from fever and chills, to irreversible and fatal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Ahmed F. Soudy
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2019
Materias:	fmd mycoplasma pcr. vaccine vsvri Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2019.62673 https://doaj.org/article/3b24b329220a40e79d0d547b19181f59
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3b24b329220a40e79d0d547b19181f59
record_format	dspace
spelling	oai:doaj.org-article:3b24b329220a40e79d0d547b19181f592021-12-02T17:04:23ZProphylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Productionhttps://dx.doi.org/10.21608/javs.2019.626731687-40722090-3308https://doaj.org/article/3b24b329220a40e79d0d547b19181f592019-04-01T00:00:00Zhttps://javs.journals.ekb.eg/article_62673.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308Mycoplasma contamination remains a major concern in the biopharmaceutical industry especially in tissue culture based viral vaccine and its presence and/or its endotoxin-like metabolites in the final products can result in pyrogenic responses ranging from fever and chills, to irreversible and fatal septic shock. This study was conducted by in vitro screening of mycoplasma in the different ingredients used in production of Foot and mouth Disease (FMD) vaccine using Polymerase Chain Reaction (PCR) using universal primers that are specific to the 16S rRNA region. Tested items include growth media, cell lines, trypsin, seed virus and working virus. Also the study evaluates the inhibitory effect of different concentrations of neomycin, kanamycin, gentamycin, polymyxin B and ciprofloxacin on mycoplasma contaminated cell lines. Our results showed that the prepared growth media, trypsin, seed virus as well as working virus were mycoplasma free and three tested cell lines were also free while another two lines were mycoplasma positive. The mycoplasma positive cell line are poorly grown in comparison with the free line using the same growth media and the virus yield from the apparently normal contaminated line was very low. Ciprofloxacin can be used for treating valuable cell line after 12 days in 25mg/L and after 18 days in 10mg/L. Ciprofloxacin plus regular antibiotic may keep the line sterile for prolonged time but treatment of contaminated cell line is not advisable. So, prophylactic control by strict personal hygiene and personal protective equipment (PPE) and adopt appropriate aseptic techniques is the core solution.Ahmed F. SoudyEgyptian Society for Animal Managementarticlefmdmycoplasmapcr. vaccinevsvriZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 4, Iss 1, Pp 30-34 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	fmd mycoplasma pcr. vaccine vsvri Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	fmd mycoplasma pcr. vaccine vsvri Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Ahmed F. Soudy Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
description	Mycoplasma contamination remains a major concern in the biopharmaceutical industry especially in tissue culture based viral vaccine and its presence and/or its endotoxin-like metabolites in the final products can result in pyrogenic responses ranging from fever and chills, to irreversible and fatal septic shock. This study was conducted by in vitro screening of mycoplasma in the different ingredients used in production of Foot and mouth Disease (FMD) vaccine using Polymerase Chain Reaction (PCR) using universal primers that are specific to the 16S rRNA region. Tested items include growth media, cell lines, trypsin, seed virus and working virus. Also the study evaluates the inhibitory effect of different concentrations of neomycin, kanamycin, gentamycin, polymyxin B and ciprofloxacin on mycoplasma contaminated cell lines. Our results showed that the prepared growth media, trypsin, seed virus as well as working virus were mycoplasma free and three tested cell lines were also free while another two lines were mycoplasma positive. The mycoplasma positive cell line are poorly grown in comparison with the free line using the same growth media and the virus yield from the apparently normal contaminated line was very low. Ciprofloxacin can be used for treating valuable cell line after 12 days in 25mg/L and after 18 days in 10mg/L. Ciprofloxacin plus regular antibiotic may keep the line sterile for prolonged time but treatment of contaminated cell line is not advisable. So, prophylactic control by strict personal hygiene and personal protective equipment (PPE) and adopt appropriate aseptic techniques is the core solution.
format	article
author	Ahmed F. Soudy
author_facet	Ahmed F. Soudy
author_sort	Ahmed F. Soudy
title	Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
title_short	Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
title_full	Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
title_fullStr	Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
title_full_unstemmed	Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production
title_sort	prophylactic control of mycoplasma contamination in starting biological materials used in viral vaccine production
publisher	Egyptian Society for Animal Management
publishDate	2019
url	https://dx.doi.org/10.21608/javs.2019.62673 https://doaj.org/article/3b24b329220a40e79d0d547b19181f59
work_keys_str_mv	AT ahmedfsoudy prophylacticcontrolofmycoplasmacontaminationinstartingbiologicalmaterialsusedinviralvaccineproduction
_version_	1718381841420910592

Prophylactic Control of Mycoplasma Contamination in Starting Biological Materials Used in Viral Vaccine Production

Ejemplares similares