Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine the diversity of oleaginous yeasts from various so...

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Autores principales: MICKY VINCENT, HUANG CHAI HUNG, PATRICIA ROWENA MARK BARAN, AFIZUL SAFWAN AZAHARI, DAYANG SALWANI AWANG ADENI
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Publicado: MBI & UNS Solo 2018
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spelling oai:doaj.org-article:3b35b785fe7a47a5a3e6a48dbd3f63ff2021-11-16T14:03:29ZIsolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia1412-033X2085-472210.13057/biodiv/d190412https://doaj.org/article/3b35b785fe7a47a5a3e6a48dbd3f63ff2018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2781https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine the diversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanning electron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 µm in width and 4-8 µm in length, typical of most unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates, targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candida krusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus (4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining. The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Further investigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provide insights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.MICKY VINCENTHUANG CHAI HUNGPATRICIA ROWENA MARK BARANAFIZUL SAFWAN AZAHARIDAYANG SALWANI AWANG ADENIMBI & UNS Soloarticlefungal lipidlipid accumulationoleaginous yeastspolymerase chain reaction (pcr)triacylglycerols (tags)Biology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1266-1272 (2018)
institution DOAJ
collection DOAJ
language EN
topic fungal lipid
lipid accumulation
oleaginous yeasts
polymerase chain reaction (pcr)
triacylglycerols (tags)
Biology (General)
QH301-705.5
spellingShingle fungal lipid
lipid accumulation
oleaginous yeasts
polymerase chain reaction (pcr)
triacylglycerols (tags)
Biology (General)
QH301-705.5
MICKY VINCENT
HUANG CHAI HUNG
PATRICIA ROWENA MARK BARAN
AFIZUL SAFWAN AZAHARI
DAYANG SALWANI AWANG ADENI
Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
description Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine the diversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanning electron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 µm in width and 4-8 µm in length, typical of most unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates, targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candida krusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus (4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining. The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Further investigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provide insights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.
format article
author MICKY VINCENT
HUANG CHAI HUNG
PATRICIA ROWENA MARK BARAN
AFIZUL SAFWAN AZAHARI
DAYANG SALWANI AWANG ADENI
author_facet MICKY VINCENT
HUANG CHAI HUNG
PATRICIA ROWENA MARK BARAN
AFIZUL SAFWAN AZAHARI
DAYANG SALWANI AWANG ADENI
author_sort MICKY VINCENT
title Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
title_short Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
title_full Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
title_fullStr Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
title_full_unstemmed Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia
title_sort isolation, identification and diversity of oleaginous yeasts from kuching, sarawak, malaysia
publisher MBI & UNS Solo
publishDate 2018
url https://doaj.org/article/3b35b785fe7a47a5a3e6a48dbd3f63ff
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