Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics

Nsp15 is a uridine specific endoribonuclease present in all coronaviruses. Here, the authors determine the cryo-EM structures of SARS-CoV-2 Nsp15 in the apo and UTP-bound states, which together with biochemical experiments, mass spectrometry and molecular dynamics simulations provide insights into t...

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Autores principales:	Monica C. Pillon, Meredith N. Frazier, Lucas B. Dillard, Jason G. Williams, Seda Kocaman, Juno M. Krahn, Lalith Perera, Cassandra K. Hayne, Jacob Gordon, Zachary D. Stewart, Mack Sobhany, Leesa J. Deterding, Allen L. Hsu, Venkata P. Dandey, Mario J. Borgnia, Robin E. Stanley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3b3bd68422cc4508a02721056de1a082
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spelling	oai:doaj.org-article:3b3bd68422cc4508a02721056de1a0822021-12-02T13:57:32ZCryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics10.1038/s41467-020-20608-z2041-1723https://doaj.org/article/3b3bd68422cc4508a02721056de1a0822021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20608-zhttps://doaj.org/toc/2041-1723Nsp15 is a uridine specific endoribonuclease present in all coronaviruses. Here, the authors determine the cryo-EM structures of SARS-CoV-2 Nsp15 in the apo and UTP-bound states, which together with biochemical experiments, mass spectrometry and molecular dynamics simulations provide insights into the catalytic mechanism of Nsp15 and its conformational dynamics.Monica C. PillonMeredith N. FrazierLucas B. DillardJason G. WilliamsSeda KocamanJuno M. KrahnLalith PereraCassandra K. HayneJacob GordonZachary D. StewartMack SobhanyLeesa J. DeterdingAllen L. HsuVenkata P. DandeyMario J. BorgniaRobin E. StanleyNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Monica C. Pillon Meredith N. Frazier Lucas B. Dillard Jason G. Williams Seda Kocaman Juno M. Krahn Lalith Perera Cassandra K. Hayne Jacob Gordon Zachary D. Stewart Mack Sobhany Leesa J. Deterding Allen L. Hsu Venkata P. Dandey Mario J. Borgnia Robin E. Stanley Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
description	Nsp15 is a uridine specific endoribonuclease present in all coronaviruses. Here, the authors determine the cryo-EM structures of SARS-CoV-2 Nsp15 in the apo and UTP-bound states, which together with biochemical experiments, mass spectrometry and molecular dynamics simulations provide insights into the catalytic mechanism of Nsp15 and its conformational dynamics.
format	article
author	Monica C. Pillon Meredith N. Frazier Lucas B. Dillard Jason G. Williams Seda Kocaman Juno M. Krahn Lalith Perera Cassandra K. Hayne Jacob Gordon Zachary D. Stewart Mack Sobhany Leesa J. Deterding Allen L. Hsu Venkata P. Dandey Mario J. Borgnia Robin E. Stanley
author_facet	Monica C. Pillon Meredith N. Frazier Lucas B. Dillard Jason G. Williams Seda Kocaman Juno M. Krahn Lalith Perera Cassandra K. Hayne Jacob Gordon Zachary D. Stewart Mack Sobhany Leesa J. Deterding Allen L. Hsu Venkata P. Dandey Mario J. Borgnia Robin E. Stanley
author_sort	Monica C. Pillon
title	Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
title_short	Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
title_full	Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
title_fullStr	Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
title_full_unstemmed	Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics
title_sort	cryo-em structures of the sars-cov-2 endoribonuclease nsp15 reveal insight into nuclease specificity and dynamics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b3bd68422cc4508a02721056de1a082
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Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics

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