Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease

Chao Xuan,1 Hui Li,1 Qing-Wu Tian,1 Jun-Jie Guo,2 Guo-Wei He,3,4 Li-Min Lun,1 Qing Wang1 1Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 2Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xuan C, Li H, Tian QW, Guo JJ, He GW, Lun LM, Wang Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	amino acid coronary artery disease myocardial infarction 4-hydroxyproline. Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/3b41278862c343f19c985de9d53cdbd3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3b41278862c343f19c985de9d53cdbd3
record_format	dspace
spelling	oai:doaj.org-article:3b41278862c343f19c985de9d53cdbd32021-12-02T18:23:04ZQuantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease1178-1998https://doaj.org/article/3b41278862c343f19c985de9d53cdbd32021-03-01T00:00:00Zhttps://www.dovepress.com/quantitative-assessment-of-serum-amino-acids-and-association-with-earl-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Chao Xuan,1 Hui Li,1 Qing-Wu Tian,1 Jun-Jie Guo,2 Guo-Wei He,3,4 Li-Min Lun,1 Qing Wang1 1Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 2Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 3Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, People’s Republic of China; 4Department of Surgery, Oregon Health and Science University, Portland, OR, USACorrespondence: Chao XuanDepartment of Clinical Laboratory, The Affiliated Hospital of Qingdao University, No. 1677, Wutai Mountain Road, Qingdao (West Coast), 266500, People’s Republic of ChinaTel +86-532-82919388Email bio-x.c@hotmail.comQing WangDepartment of Clinical Laboratory, The Affiliated Hospital of Qingdao University, No. 59, Haier Road, Qingdao, 266100, People’s Republic of ChinaTel +86-532-82913085Email wangqing0533@yeah.netBackground: Amino acids play essential roles in protein construction and metabolism. Our study aims to provide a profile of amino acid changes in the serum of patients with early-onset coronary artery disease (EOCAD) and identify potential disease biomarkers.Methods: Ultra-performance liquid chromatography-multiple reaction monitoring-multistage/mass spectrometry (UPLC-MRM-MS/MS) was used to determine the amino acid profile of patients with EOCAD in sample pools. In the validation stage, the serum levels of candidate amino acids of interest are determined for each sample.Results: A total of 128 EOCAD patients and 64 healthy controls were included in the study. Eight serum amino acids associated with disease state were identified. Compared with the control group, serum levels of seven amino acids (L-Arginine, L-Methionine, L-Tyrosine, L-Serine, L-Aspartic acid, L-Phenylalanine, and L-Glutamic acid) increased and one (4-Hydroxyproline) decreased in the patient group. Results from the validation stage demonstrate that serum levels of 4-Hydroxyproline were significantly lower in myocardial infarction (MI) patients (9.889 ± 3.635 μg/mL) than those in the controls (16.433 ± 4.562 μmol/L, p < 0.001). Elevated serum 4-Hydroxyproline levels were shown to be an independent protective factor for MI (OR = 0.863, 95% CI: 0.822– 0.901). The significant negative correlation was seen between serum 4-Hydroxyproline levels and cardiac troponin I (r = − 0.667) in MI patients.Conclusion: We have provided a serum amino acid profile for EOCAD patients and screened eight disease state-related amino acids, and we have also shown that 4-Hydroxyproline is a promising target for further biomarker studies in early-onset MI.Keywords: amino acid, coronary artery disease, myocardial infarction, 4-HydroxyprolineXuan CLi HTian QWGuo JJHe GWLun LMWang QDove Medical Pressarticleamino acidcoronary artery diseasemyocardial infarction4-hydroxyproline.GeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 16, Pp 465-474 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amino acid coronary artery disease myocardial infarction 4-hydroxyproline. Geriatrics RC952-954.6
spellingShingle	amino acid coronary artery disease myocardial infarction 4-hydroxyproline. Geriatrics RC952-954.6 Xuan C Li H Tian QW Guo JJ He GW Lun LM Wang Q Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
description	Chao Xuan,1 Hui Li,1 Qing-Wu Tian,1 Jun-Jie Guo,2 Guo-Wei He,3,4 Li-Min Lun,1 Qing Wang1 1Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 2Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 3Center for Basic Medical Research & Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, People’s Republic of China; 4Department of Surgery, Oregon Health and Science University, Portland, OR, USACorrespondence: Chao XuanDepartment of Clinical Laboratory, The Affiliated Hospital of Qingdao University, No. 1677, Wutai Mountain Road, Qingdao (West Coast), 266500, People’s Republic of ChinaTel +86-532-82919388Email bio-x.c@hotmail.comQing WangDepartment of Clinical Laboratory, The Affiliated Hospital of Qingdao University, No. 59, Haier Road, Qingdao, 266100, People’s Republic of ChinaTel +86-532-82913085Email wangqing0533@yeah.netBackground: Amino acids play essential roles in protein construction and metabolism. Our study aims to provide a profile of amino acid changes in the serum of patients with early-onset coronary artery disease (EOCAD) and identify potential disease biomarkers.Methods: Ultra-performance liquid chromatography-multiple reaction monitoring-multistage/mass spectrometry (UPLC-MRM-MS/MS) was used to determine the amino acid profile of patients with EOCAD in sample pools. In the validation stage, the serum levels of candidate amino acids of interest are determined for each sample.Results: A total of 128 EOCAD patients and 64 healthy controls were included in the study. Eight serum amino acids associated with disease state were identified. Compared with the control group, serum levels of seven amino acids (L-Arginine, L-Methionine, L-Tyrosine, L-Serine, L-Aspartic acid, L-Phenylalanine, and L-Glutamic acid) increased and one (4-Hydroxyproline) decreased in the patient group. Results from the validation stage demonstrate that serum levels of 4-Hydroxyproline were significantly lower in myocardial infarction (MI) patients (9.889 ± 3.635 μg/mL) than those in the controls (16.433 ± 4.562 μmol/L, p < 0.001). Elevated serum 4-Hydroxyproline levels were shown to be an independent protective factor for MI (OR = 0.863, 95% CI: 0.822– 0.901). The significant negative correlation was seen between serum 4-Hydroxyproline levels and cardiac troponin I (r = − 0.667) in MI patients.Conclusion: We have provided a serum amino acid profile for EOCAD patients and screened eight disease state-related amino acids, and we have also shown that 4-Hydroxyproline is a promising target for further biomarker studies in early-onset MI.Keywords: amino acid, coronary artery disease, myocardial infarction, 4-Hydroxyproline
format	article
author	Xuan C Li H Tian QW Guo JJ He GW Lun LM Wang Q
author_facet	Xuan C Li H Tian QW Guo JJ He GW Lun LM Wang Q
author_sort	Xuan C
title	Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
title_short	Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
title_full	Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
title_fullStr	Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
title_full_unstemmed	Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease
title_sort	quantitative assessment of serum amino acids and association with early-onset coronary artery disease
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/3b41278862c343f19c985de9d53cdbd3
work_keys_str_mv	AT xuanc quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT lih quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT tianqw quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT guojj quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT hegw quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT lunlm quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease AT wangq quantitativeassessmentofserumaminoacidsandassociationwithearlyonsetcoronaryarterydisease
_version_	1718378089205989376

Quantitative Assessment of Serum Amino Acids and Association with Early-Onset Coronary Artery Disease

Ejemplares similares