The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

Abstract The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein famil...

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Autores principales:	Majeed Bakari-Soale, Nonso Josephat Ikenga, Marion Scheibe, Falk Butter, Nicola G. Jones, Susanne Kramer, Markus Engstler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b43891eae7f45c49cbce7862a35af9c
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spelling	oai:doaj.org-article:3b43891eae7f45c49cbce7862a35af9c2021-12-02T18:50:03ZThe nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei10.1038/s41598-021-97020-02045-2322https://doaj.org/article/3b43891eae7f45c49cbce7862a35af9c2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97020-0https://doaj.org/toc/2045-2322Abstract The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.Majeed Bakari-SoaleNonso Josephat IkengaMarion ScheibeFalk ButterNicola G. JonesSusanne KramerMarkus EngstlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Majeed Bakari-Soale Nonso Josephat Ikenga Marion Scheibe Falk Butter Nicola G. Jones Susanne Kramer Markus Engstler The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
description	Abstract The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process.
format	article
author	Majeed Bakari-Soale Nonso Josephat Ikenga Marion Scheibe Falk Butter Nicola G. Jones Susanne Kramer Markus Engstler
author_facet	Majeed Bakari-Soale Nonso Josephat Ikenga Marion Scheibe Falk Butter Nicola G. Jones Susanne Kramer Markus Engstler
author_sort	Majeed Bakari-Soale
title	The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
title_short	The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
title_full	The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
title_fullStr	The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
title_full_unstemmed	The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
title_sort	nucleolar dexd/h protein hel66 is involved in ribosome biogenesis in trypanosoma brucei
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b43891eae7f45c49cbce7862a35af9c
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The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei

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