Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals

Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic carcinogenic compounds and non-carcinogenic compounds on the expression and functionality of connexin-b...

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Autores principales:	Kaat Leroy, Alanah Pieters, Axelle Cooreman, Raf Van Campenhout, Bruno Cogliati, Mathieu Vinken
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	connexin hemichannel gap junction genotoxic non-genotoxic carcinogen Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/3b4681c2e07e4da8bba08f8e6243ce5a
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spelling	oai:doaj.org-article:3b4681c2e07e4da8bba08f8e6243ce5a2021-11-11T17:11:02ZConnexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals10.3390/ijms2221117241422-00671661-6596https://doaj.org/article/3b4681c2e07e4da8bba08f8e6243ce5a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11724https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic carcinogenic compounds and non-carcinogenic compounds on the expression and functionality of connexin-based channels, both gap junctions and connexin hemichannels, were investigated in human hepatoma HepaRG cell cultures. Expression of connexin26, connexin32, and connexin43 was evaluated by means of real-time reverse transcription quantitative polymerase chain reaction analysis, immunoblot analysis and in situ immunostaining. Gap junction functionality was assessed via a scrape loading/dye transfer assay. Opening of connexin hemichannels was monitored by measuring extracellular release of adenosine triphosphate. It was found that both genotoxic and non-genotoxic carcinogenic compounds negatively affect connexin32 expression. However, no specific effects related to chemical type were observed at gap junction or connexin hemichannel functionality level.Kaat LeroyAlanah PietersAxelle CooremanRaf Van CampenhoutBruno CogliatiMathieu VinkenMDPI AGarticleconnexinhemichannelgap junctiongenotoxicnon-genotoxiccarcinogenBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11724, p 11724 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	connexin hemichannel gap junction genotoxic non-genotoxic carcinogen Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	connexin hemichannel gap junction genotoxic non-genotoxic carcinogen Biology (General) QH301-705.5 Chemistry QD1-999 Kaat Leroy Alanah Pieters Axelle Cooreman Raf Van Campenhout Bruno Cogliati Mathieu Vinken Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
description	Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic carcinogenic compounds and non-carcinogenic compounds on the expression and functionality of connexin-based channels, both gap junctions and connexin hemichannels, were investigated in human hepatoma HepaRG cell cultures. Expression of connexin26, connexin32, and connexin43 was evaluated by means of real-time reverse transcription quantitative polymerase chain reaction analysis, immunoblot analysis and in situ immunostaining. Gap junction functionality was assessed via a scrape loading/dye transfer assay. Opening of connexin hemichannels was monitored by measuring extracellular release of adenosine triphosphate. It was found that both genotoxic and non-genotoxic carcinogenic compounds negatively affect connexin32 expression. However, no specific effects related to chemical type were observed at gap junction or connexin hemichannel functionality level.
format	article
author	Kaat Leroy Alanah Pieters Axelle Cooreman Raf Van Campenhout Bruno Cogliati Mathieu Vinken
author_facet	Kaat Leroy Alanah Pieters Axelle Cooreman Raf Van Campenhout Bruno Cogliati Mathieu Vinken
author_sort	Kaat Leroy
title	Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
title_short	Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
title_full	Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
title_fullStr	Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
title_full_unstemmed	Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals
title_sort	connexin-based channel activity is not specifically altered by hepatocarcinogenic chemicals
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3b4681c2e07e4da8bba08f8e6243ce5a
work_keys_str_mv	AT kaatleroy connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals AT alanahpieters connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals AT axellecooreman connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals AT rafvancampenhout connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals AT brunocogliati connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals AT mathieuvinken connexinbasedchannelactivityisnotspecificallyalteredbyhepatocarcinogenicchemicals
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Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals

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