Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.

In this study, mRNA-Seq was used to characterize and compare the leukocyte transcriptomes from two taurine breeds (Holstein and Jersey), and one indicine breed (Cholistani). At the genomic level, we identified breed-specific base changes in protein coding regions. Among 7,793,425 coding bases, only...

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Autores principales:	Wen Huang, Asif Nadeem, Bao Zhang, Masroor Babar, Morris Soller, Hasan Khatib
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3b4aad23c6ce48b896b460cbb243d531
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spelling	oai:doaj.org-article:3b4aad23c6ce48b896b460cbb243d5312021-11-18T07:29:38ZCharacterization and comparison of the leukocyte transcriptomes of three cattle breeds.1932-620310.1371/journal.pone.0030244https://doaj.org/article/3b4aad23c6ce48b896b460cbb243d5312012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22291923/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, mRNA-Seq was used to characterize and compare the leukocyte transcriptomes from two taurine breeds (Holstein and Jersey), and one indicine breed (Cholistani). At the genomic level, we identified breed-specific base changes in protein coding regions. Among 7,793,425 coding bases, only 165 differed between Holstein and Jersey, and 3,383 (0.04%) differed between Holstein and Cholistani, 817 (25%) of which resulted in amino acid changes in 627 genes. At the transcriptional level, we assembled transcripts and estimated their abundances including those from more than 3,000 unannotated intergeneic regions. Differential gene expression analysis showed a high similarity between Holstein and Jersey, and a much greater difference between the taurine breeds and the indicine breed. We identified gene ontology pathways that were systematically altered, including the electron transport chain and immune response pathways that may contribute to different levels of heat tolerance and disease resistance in taurine and indicine breeds. At the post-transcriptional level, sequencing mRNA allowed us to identify a number of genes undergoing differential alternative splicing among different breeds. This study provided a high-resolution survey of the variation between bovine transcriptomes at different levels and may provide important biological insights into the phenotypic differentiation among cattle breeds.Wen HuangAsif NadeemBao ZhangMasroor BabarMorris SollerHasan KhatibPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30244 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wen Huang Asif Nadeem Bao Zhang Masroor Babar Morris Soller Hasan Khatib Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
description	In this study, mRNA-Seq was used to characterize and compare the leukocyte transcriptomes from two taurine breeds (Holstein and Jersey), and one indicine breed (Cholistani). At the genomic level, we identified breed-specific base changes in protein coding regions. Among 7,793,425 coding bases, only 165 differed between Holstein and Jersey, and 3,383 (0.04%) differed between Holstein and Cholistani, 817 (25%) of which resulted in amino acid changes in 627 genes. At the transcriptional level, we assembled transcripts and estimated their abundances including those from more than 3,000 unannotated intergeneic regions. Differential gene expression analysis showed a high similarity between Holstein and Jersey, and a much greater difference between the taurine breeds and the indicine breed. We identified gene ontology pathways that were systematically altered, including the electron transport chain and immune response pathways that may contribute to different levels of heat tolerance and disease resistance in taurine and indicine breeds. At the post-transcriptional level, sequencing mRNA allowed us to identify a number of genes undergoing differential alternative splicing among different breeds. This study provided a high-resolution survey of the variation between bovine transcriptomes at different levels and may provide important biological insights into the phenotypic differentiation among cattle breeds.
format	article
author	Wen Huang Asif Nadeem Bao Zhang Masroor Babar Morris Soller Hasan Khatib
author_facet	Wen Huang Asif Nadeem Bao Zhang Masroor Babar Morris Soller Hasan Khatib
author_sort	Wen Huang
title	Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
title_short	Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
title_full	Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
title_fullStr	Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
title_full_unstemmed	Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
title_sort	characterization and comparison of the leukocyte transcriptomes of three cattle breeds.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/3b4aad23c6ce48b896b460cbb243d531
work_keys_str_mv	AT wenhuang characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds AT asifnadeem characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds AT baozhang characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds AT masroorbabar characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds AT morrissoller characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds AT hasankhatib characterizationandcomparisonoftheleukocytetranscriptomesofthreecattlebreeds
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Characterization and comparison of the leukocyte transcriptomes of three cattle breeds.

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