A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION

Arginine deiminase is a bacterial enzyme that hydrolyses arginine with citrulline and ammonia formation. In recent years, increasing evidence is reported about in vitro and in vivo anti-angiogenic action of arginine deiminase from Mycoplasma spp. Our studies have shown that arginine deiminase from S...

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Autores principales:	E. A. Starikova, A. B. Karaseva, L. A. Burova, A. N. Suvorov, A. V. Sokolov, V. B. Vasilyev, I. S. Freidlin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	s. pyogenes arginine deiminase endothelial cells arginine proliferation Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/3b53e097c00849ddb5b4547dcfe479ec
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spelling	oai:doaj.org-article:3b53e097c00849ddb5b4547dcfe479ec2021-11-18T08:03:45ZA ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION1563-06252313-741X10.15789/1563-0625-2016-6-555-562https://doaj.org/article/3b53e097c00849ddb5b4547dcfe479ec2016-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1136https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XArginine deiminase is a bacterial enzyme that hydrolyses arginine with citrulline and ammonia formation. In recent years, increasing evidence is reported about in vitro and in vivo anti-angiogenic action of arginine deiminase from Mycoplasma spp. Our studies have shown that arginine deiminase from Streptococcus pyogenes M22 exerts similar effects, i.e., inhibits proliferation and other endothelial cell functions related to angiogenesis. To confirm a leading role of arginine deiminase, as a factor responsible for the anti-proliferative effect, we have constructed an isogenic S. pyogenes M49-16 mutant unable to express arginine deiminase. A comparative analysis of anti-proliferative activity of original S. pyogenes M49-16 strain and its isogenic mutant with arginine deiminase gene deletion (M49-16delAD) was performed, using an endothelial EA.hy926 cell line. The bacterial supernatantes obtained by sonication of S. pyogenes M49-16 and M49-16delAD were tested. The ability of S. pyogenes M49-16 and M49-16delAD supernatantes to hydrolyze arginine was assessed. Moreover, we compared effects of the Streptococcus supernatantes upon proliferative activity of endothelial cells and their distribution through the cell cycle phases.Supernatantes from original S. pyogenes 49-16 strain were shown to inhibit endothelial cell proliferation to a significant degree (down to 50% of controls). This effect was due to its arginine hydrolyzing activity, i. e. addition of exogenous arginine to the medium resulted into recovery of the cell proliferation levels. The supernatante from S. pyogenes M49-16delAD showed a lower ability to hydrolyze arginine as compared to the supernatante of original strain. Culturing of endothelial cells supplied with S. pyogenes M49-16delAD supernatantes resulted into reduction of their proliferative activity by 10% of control values. Analysis of the cell cycle distribution was concordant with these results. S. pyogenes M49-16 supernatante caused a decrease in S-phase cell fraction by 20% against controls. With a supernatants from S. pyogenes M49-16delAD, such drop in DNA-synthesizing cell ratio was significantly weaker (by only 5% of the control). These results reveal new pathogenetic mechanisms of endothelial dysfunction during streptococcal infection and suggest anti-angiogenic potential of streptococcal arginine deiminase.E. A. StarikovaA. B. KarasevaL. A. BurovaA. N. SuvorovA. V. SokolovV. B. VasilyevI. S. FreidlinSPb RAACIarticles. pyogenesarginine deiminaseendothelial cellsarginineproliferationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 6, Pp 555-562 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	s. pyogenes arginine deiminase endothelial cells arginine proliferation Immunologic diseases. Allergy RC581-607
spellingShingle	s. pyogenes arginine deiminase endothelial cells arginine proliferation Immunologic diseases. Allergy RC581-607 E. A. Starikova A. B. Karaseva L. A. Burova A. N. Suvorov A. V. Sokolov V. B. Vasilyev I. S. Freidlin A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
description	Arginine deiminase is a bacterial enzyme that hydrolyses arginine with citrulline and ammonia formation. In recent years, increasing evidence is reported about in vitro and in vivo anti-angiogenic action of arginine deiminase from Mycoplasma spp. Our studies have shown that arginine deiminase from Streptococcus pyogenes M22 exerts similar effects, i.e., inhibits proliferation and other endothelial cell functions related to angiogenesis. To confirm a leading role of arginine deiminase, as a factor responsible for the anti-proliferative effect, we have constructed an isogenic S. pyogenes M49-16 mutant unable to express arginine deiminase. A comparative analysis of anti-proliferative activity of original S. pyogenes M49-16 strain and its isogenic mutant with arginine deiminase gene deletion (M49-16delAD) was performed, using an endothelial EA.hy926 cell line. The bacterial supernatantes obtained by sonication of S. pyogenes M49-16 and M49-16delAD were tested. The ability of S. pyogenes M49-16 and M49-16delAD supernatantes to hydrolyze arginine was assessed. Moreover, we compared effects of the Streptococcus supernatantes upon proliferative activity of endothelial cells and their distribution through the cell cycle phases.Supernatantes from original S. pyogenes 49-16 strain were shown to inhibit endothelial cell proliferation to a significant degree (down to 50% of controls). This effect was due to its arginine hydrolyzing activity, i. e. addition of exogenous arginine to the medium resulted into recovery of the cell proliferation levels. The supernatante from S. pyogenes M49-16delAD showed a lower ability to hydrolyze arginine as compared to the supernatante of original strain. Culturing of endothelial cells supplied with S. pyogenes M49-16delAD supernatantes resulted into reduction of their proliferative activity by 10% of control values. Analysis of the cell cycle distribution was concordant with these results. S. pyogenes M49-16 supernatante caused a decrease in S-phase cell fraction by 20% against controls. With a supernatants from S. pyogenes M49-16delAD, such drop in DNA-synthesizing cell ratio was significantly weaker (by only 5% of the control). These results reveal new pathogenetic mechanisms of endothelial dysfunction during streptococcal infection and suggest anti-angiogenic potential of streptococcal arginine deiminase.
format	article
author	E. A. Starikova A. B. Karaseva L. A. Burova A. N. Suvorov A. V. Sokolov V. B. Vasilyev I. S. Freidlin
author_facet	E. A. Starikova A. B. Karaseva L. A. Burova A. N. Suvorov A. V. Sokolov V. B. Vasilyev I. S. Freidlin
author_sort	E. A. Starikova
title	A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
title_short	A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
title_full	A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
title_fullStr	A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
title_full_unstemmed	A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION
title_sort	role of arginine deiminase from streptococcus pyogenes m49-16 in promoting infection and inhibition of endothelial cell proliferation
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/3b53e097c00849ddb5b4547dcfe479ec
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A ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES M49-16 IN PROMOTING INFECTION AND INHIBITION OF ENDOTHELIAL CELL PROLIFERATION

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