Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.

Tropical forests represent vast carbon stocks and continue to be key carbon sinks and buffer climate changes. The international policy constructed several mechanisms aiming at conservation and sustainable use of these forests. Illegal logging is an important threat of forests, especially in the trop...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maurizio Mascarello, Mario Amalfi, Pieter Asselman, Erik Smets, Olivier J Hardy, Hans Beeckman, Steven B Janssens
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/3b5ae8346fc0430489964b25d8551e6b
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:3b5ae8346fc0430489964b25d8551e6b
record_format dspace
spelling oai:doaj.org-article:3b5ae8346fc0430489964b25d8551e6b2021-11-25T06:23:33ZGenome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.1932-620310.1371/journal.pone.0251655https://doaj.org/article/3b5ae8346fc0430489964b25d8551e6b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251655https://doaj.org/toc/1932-6203Tropical forests represent vast carbon stocks and continue to be key carbon sinks and buffer climate changes. The international policy constructed several mechanisms aiming at conservation and sustainable use of these forests. Illegal logging is an important threat of forests, especially in the tropics. Several laws and regulations have been set up to combat illegal timber trade. Despite significant enforcement efforts of these regulations, illegal logging continues to be a serious problem and impacts for the functioning of the forest ecosystem and global biodiversity in the tropics. Microscopic analysis of wood samples and the use of conventional plant DNA barcodes often do not allow to distinguish closely-related species. The use of novel molecular technologies could make an important contribution for the identification of tree species. In this study, we used high-throughput sequencing technologies and bioinformatics tools to obtain the complete de-novo chloroplast genome of 62 commercial African timber species using the genome skimming method. Then, we performed a comparative genomic analysis that revealed new candidate genetic regions for the discrimination of closely-related species. We concluded that genome skimming is a promising method for the development of plant genetic markers to combat illegal logging activities supporting CITES, FLEGT and the EU Timber Regulation.Maurizio MascarelloMario AmalfiPieter AsselmanErik SmetsOlivier J HardyHans BeeckmanSteven B JanssensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251655 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Maurizio Mascarello
Mario Amalfi
Pieter Asselman
Erik Smets
Olivier J Hardy
Hans Beeckman
Steven B Janssens
Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
description Tropical forests represent vast carbon stocks and continue to be key carbon sinks and buffer climate changes. The international policy constructed several mechanisms aiming at conservation and sustainable use of these forests. Illegal logging is an important threat of forests, especially in the tropics. Several laws and regulations have been set up to combat illegal timber trade. Despite significant enforcement efforts of these regulations, illegal logging continues to be a serious problem and impacts for the functioning of the forest ecosystem and global biodiversity in the tropics. Microscopic analysis of wood samples and the use of conventional plant DNA barcodes often do not allow to distinguish closely-related species. The use of novel molecular technologies could make an important contribution for the identification of tree species. In this study, we used high-throughput sequencing technologies and bioinformatics tools to obtain the complete de-novo chloroplast genome of 62 commercial African timber species using the genome skimming method. Then, we performed a comparative genomic analysis that revealed new candidate genetic regions for the discrimination of closely-related species. We concluded that genome skimming is a promising method for the development of plant genetic markers to combat illegal logging activities supporting CITES, FLEGT and the EU Timber Regulation.
format article
author Maurizio Mascarello
Mario Amalfi
Pieter Asselman
Erik Smets
Olivier J Hardy
Hans Beeckman
Steven B Janssens
author_facet Maurizio Mascarello
Mario Amalfi
Pieter Asselman
Erik Smets
Olivier J Hardy
Hans Beeckman
Steven B Janssens
author_sort Maurizio Mascarello
title Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
title_short Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
title_full Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
title_fullStr Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
title_full_unstemmed Genome skimming reveals novel plastid markers for the molecular identification of illegally logged African timber species.
title_sort genome skimming reveals novel plastid markers for the molecular identification of illegally logged african timber species.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/3b5ae8346fc0430489964b25d8551e6b
work_keys_str_mv AT mauriziomascarello genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT marioamalfi genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT pieterasselman genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT eriksmets genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT olivierjhardy genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT hansbeeckman genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
AT stevenbjanssens genomeskimmingrevealsnovelplastidmarkersforthemolecularidentificationofillegallyloggedafricantimberspecies
_version_ 1718413796585766912