Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage

Abstract Cholangiocarcinoma (CCA) is a malignant disease with a poor prognosis, and several studies have been conducted using different molecular markers as a tool for CCA diagnosis, including Clonorchis sinensis (CS)-CCA. We initially identified the expression profiles of the three markers of inter...

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Autores principales:	Nattawan Suwannakul, Kaoru Midorikawa, Chunping Du, Ya-Peng Qi, Jie Zhang, Bang-De Xiang, Mariko Murata, Ning Ma
Formato:	article
Lenguaje:	EN
Publicado:	Springer 2021
Materias:	Cholangiocarcinoma Subcellular localization HMGB1 SOX9 YAP1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/3b6a41d34b9347a18f7791ae260592ff
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spelling	oai:doaj.org-article:3b6a41d34b9347a18f7791ae260592ff2021-11-14T12:44:39ZSubcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage10.1007/s12672-021-00446-12730-6011https://doaj.org/article/3b6a41d34b9347a18f7791ae260592ff2021-11-01T00:00:00Zhttps://doi.org/10.1007/s12672-021-00446-1https://doaj.org/toc/2730-6011Abstract Cholangiocarcinoma (CCA) is a malignant disease with a poor prognosis, and several studies have been conducted using different molecular markers as a tool for CCA diagnosis, including Clonorchis sinensis (CS)-CCA. We initially identified the expression profiles of the three markers of interest, HMGB1, SOX9, and YAP1, using GSE (GSE76297 and GSE32958) datasets. Upregulated levels of these three proteins were detected in CCA samples compared to those in normal samples. To clarify this issue, 24 human CCA tissues with paired adjacent normal tissues were evaluated using immunohistochemical staining. Of the three markers, the total cellular staining intensities were scanned, and subcellular localization was scored in the nuclear and cytoplasmic regions. The intensities of HMGB1, SOX9, and YAP1 were elevated in CCA tissues than the adjacent normal tissues. Individual scoring of subcellular localization revealed that the expression levels of HMGB1 (nucleus) and YAP1 (nucleus and cytoplasm) were significantly different from the pathologic M stage. Moreover, the translocation pattern was categorized using “site-index”, and the results demonstrated that the overexpression of HMGB1 and SOX9 was mostly observed in both the nucleus and cytoplasm, whereas YAP1 was predominantly expressed in the cytoplasm of tumor cells. Interestingly, the site index of HMGB1 was moderately correlated with the tumor stage (r = 0.441, p = 0.031). These findings imply that the overexpression of subcellular HMGB1 could be associated with the metastatic status of patients with CS-CCA, which was shown to be effective for CS-CCA prognosis.Nattawan SuwannakulKaoru MidorikawaChunping DuYa-Peng QiJie ZhangBang-De XiangMariko MurataNing MaSpringerarticleCholangiocarcinomaSubcellular localizationHMGB1SOX9YAP1Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENDiscover Oncology, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cholangiocarcinoma Subcellular localization HMGB1 SOX9 YAP1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Cholangiocarcinoma Subcellular localization HMGB1 SOX9 YAP1 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Nattawan Suwannakul Kaoru Midorikawa Chunping Du Ya-Peng Qi Jie Zhang Bang-De Xiang Mariko Murata Ning Ma Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
description	Abstract Cholangiocarcinoma (CCA) is a malignant disease with a poor prognosis, and several studies have been conducted using different molecular markers as a tool for CCA diagnosis, including Clonorchis sinensis (CS)-CCA. We initially identified the expression profiles of the three markers of interest, HMGB1, SOX9, and YAP1, using GSE (GSE76297 and GSE32958) datasets. Upregulated levels of these three proteins were detected in CCA samples compared to those in normal samples. To clarify this issue, 24 human CCA tissues with paired adjacent normal tissues were evaluated using immunohistochemical staining. Of the three markers, the total cellular staining intensities were scanned, and subcellular localization was scored in the nuclear and cytoplasmic regions. The intensities of HMGB1, SOX9, and YAP1 were elevated in CCA tissues than the adjacent normal tissues. Individual scoring of subcellular localization revealed that the expression levels of HMGB1 (nucleus) and YAP1 (nucleus and cytoplasm) were significantly different from the pathologic M stage. Moreover, the translocation pattern was categorized using “site-index”, and the results demonstrated that the overexpression of HMGB1 and SOX9 was mostly observed in both the nucleus and cytoplasm, whereas YAP1 was predominantly expressed in the cytoplasm of tumor cells. Interestingly, the site index of HMGB1 was moderately correlated with the tumor stage (r = 0.441, p = 0.031). These findings imply that the overexpression of subcellular HMGB1 could be associated with the metastatic status of patients with CS-CCA, which was shown to be effective for CS-CCA prognosis.
format	article
author	Nattawan Suwannakul Kaoru Midorikawa Chunping Du Ya-Peng Qi Jie Zhang Bang-De Xiang Mariko Murata Ning Ma
author_facet	Nattawan Suwannakul Kaoru Midorikawa Chunping Du Ya-Peng Qi Jie Zhang Bang-De Xiang Mariko Murata Ning Ma
author_sort	Nattawan Suwannakul
title	Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
title_short	Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
title_full	Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
title_fullStr	Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
title_full_unstemmed	Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage
title_sort	subcellular localization of hmgb1 in human cholangiocarcinoma: correlation with tumor stage
publisher	Springer
publishDate	2021
url	https://doaj.org/article/3b6a41d34b9347a18f7791ae260592ff
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Subcellular localization of HMGB1 in human cholangiocarcinoma: correlation with tumor stage

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